| 产品: | POLR3B 抗体 |
| 货号: | AF9165 |
| 描述: | Rabbit polyclonal antibody to POLR3B |
| 应用: | WB IF/ICC |
| 反应: | Human, Rat |
| 预测: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 128kDa; 128kD(Calculated). |
| 蛋白号: | Q9NW08 |
| RRID: | AB_2843355 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9165, RRID:AB_2843355.
展开/折叠
C128; DNA directed RNA polymerase III 127.6 kDa polypeptide; DNA directed RNA polymerase III subunit B; DNA-directed RNA polymerase III 127.6 kDa polypeptide; DNA-directed RNA polymerase III subunit B; DNA-directed RNA polymerase III subunit RPC2; FLJ10388; polr3b; Polymerase (RNA) III (DNA directed) polypeptide B; RNA polymerase III subunit C2; RNA polymerase III subunit RPC2; RPC2; RPC2_HUMAN;
抗原和靶标
- Q9NW08 RPC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVLAEEFGNLTPEQLAAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGAVGASVTSSTHEKKSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIVKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVNTFRLMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIEFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHGYNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLSKYNE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NW08 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T12 | Phosphorylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K290 | Ubiquitination | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| K601 | Ubiquitination | Uniprot | |
| K609 | Ubiquitination | Uniprot | |
| S680 | Phosphorylation | Uniprot | |
| K691 | Ubiquitination | Uniprot | |
| T707 | Phosphorylation | Uniprot | |
| Y710 | Phosphorylation | Uniprot | |
| Y714 | Phosphorylation | Uniprot | |
| T722 | Phosphorylation | Uniprot | |
| K723 | Ubiquitination | Uniprot | |
| S816 | Phosphorylation | Uniprot | |
| K820 | Ubiquitination | Uniprot | |
| K824 | Ubiquitination | Uniprot | |
| K830 | Ubiquitination | Uniprot | |
| K852 | Ubiquitination | Uniprot | |
| K859 | Methylation | Uniprot | |
| K859 | Ubiquitination | Uniprot | |
| S872 | Phosphorylation | Uniprot | |
| S873 | Phosphorylation | Uniprot | |
| K896 | Ubiquitination | Uniprot | |
| K950 | Ubiquitination | Uniprot | |
| K968 | Ubiquitination | Uniprot | |
| K970 | Ubiquitination | Uniprot | |
| K1013 | Methylation | Uniprot | |
| K1019 | Ubiquitination | Uniprot | |
| T1036 | Phosphorylation | Uniprot |
研究背景
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Nucleus.
Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.
Belongs to the RNA polymerase beta chain family.
研究领域
· Genetic Information Processing > Transcription > RNA polymerase.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)
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