产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9169, RRID:AB_2843359.
展开/折叠
DKFZp566J153; hPrp 31; hPrp31; NY BR 99; Pre mRNA processing factor 31; Pre mRNA processing factor 31 homolog (yeast); Pre mRNA processing factor 31 homolog; Pre-mRNA-processing factor 31; Precursor mRNA-processing factor 31, S. cerevisiae, homolog of; Protein 61K; PRP 31; PRP31; PRP31 pre mRNA processing factor 31 homolog (yeast); PRP31 pre mRNA processing factor 31 homolog; PRP31_HUMAN; PRPF 31; prpf31; RP 11; RP11; Serologically defined breast cancer antigen NY BR 99; Serologically defined breast cancer antigen NY-BR-99; SNRNP61; U4/U6 small nuclear ribonucleoprotein Prp31; U4/U6 snRNP 61 kDa protein;
抗原和靶标
- Q8WWY3 PRP31_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLADELLADLEEAAEEEEGGSYGEEEEEPAIEDVQEETQLDLSGDSVKTIAKLWDSKMFAEIMMKIEEYISKQAKASEVMGPVEAAPEYRVIVDANNLTVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQGQQLSEEELERLEEACDMALELNASKHRIYEYVESRMSFIAPNLSIIIGASTAAKIMGVAGGLTNLSKMPACNIMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLRRKAARLVAAKCTLAARVDSFHESTEGKVGYELKDEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGGRRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRVRQTQVNEATKARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEANQKYFSSMAEFLKVKGEKSGLMST
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8WWY3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K53 | Ubiquitination | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| Y90 | Phosphorylation | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| Y205 | Phosphorylation | Uniprot | |
| Y207 | Phosphorylation | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| Y273 | Phosphorylation | Uniprot | |
| K298 | Sumoylation | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| K321 | Sumoylation | Uniprot | |
| K321 | Ubiquitination | Uniprot | |
| K327 | Sumoylation | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| K338 | Sumoylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| S379 | Phosphorylation | Uniprot | |
| Y388 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| T410 | Phosphorylation | Uniprot | |
| T416 | Phosphorylation | Uniprot | |
| K417 | Sumoylation | Uniprot | |
| K417 | Ubiquitination | Uniprot | |
| K422 | Sumoylation | Uniprot | |
| K430 | Sumoylation | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| Y435 | Phosphorylation | Uniprot | |
| K438 | Acetylation | Uniprot | |
| K438 | Methylation | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| T440 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S446 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T448 | Phosphorylation | Uniprot | |
| S450 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S451 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T455 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K470 | Acetylation | Uniprot | |
| K478 | Sumoylation | Uniprot | |
| K478 | Ubiquitination | Uniprot | |
| Y479 | Phosphorylation | Uniprot | |
| S481 | Phosphorylation | Uniprot | |
| S482 | Phosphorylation | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| T499 | Phosphorylation | Uniprot |
研究背景
Involved in pre-mRNA splicing as component of the spliceosome. Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.
Nucleus. Nucleus speckle. Nucleus>Cajal body.
Note: Predominantly found in speckles and in Cajal bodies.
Ubiquitously expressed.
Identified in the spliceosome B complex. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Interacts with a complex formed by SNU13 and U4 snRNA, but not with SNU13 or U4 snRNA alone. The complex formed by SNU13 and PRPF31 binds also U4atac snRNA, a characteristic component of specific, less abundant spliceosomal complexes. Interacts with PRPF6/U5 snRNP-associated 102 kDa protein. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts (via its NLS) with CTNNBL1.
Interacts with the snRNP via the Nop domain.
The coiled coil domain is formed by two non-contiguous helices.
Belongs to the PRP31 family.
研究领域
· Genetic Information Processing > Transcription > Spliceosome.
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