产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9173, RRID:AB_2843363.
展开/折叠
GTP-binding protein ypt3; H YPT3; RAB11B; RAB11B member RAS oncogene family; Ras-related protein Rab-11B; RB11B_HUMAN; YPT3;
抗原和靶标
- Q15907 RB11B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNNLSFIETSALDSTNVEEAFKNILTEIYRIVSQKQIADRAAHDESPGNNVVDISVPPTTDGQKPNKLQCCQNL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15907 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T3 | Phosphorylation | Uniprot | |
| Y8 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| K24 | Methylation | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| T43 | Phosphorylation | Uniprot | |
| T50 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| R74 | Methylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| Y91 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| T98 | Phosphorylation | Uniprot | |
| Y99 | Phosphorylation | Uniprot | |
| R104 | Methylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| R132 | Methylation | Uniprot | |
| T136 | Phosphorylation | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| Y173 | Phosphorylation | Uniprot | |
| K179 | Ubiquitination | Uniprot |
研究背景
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11B plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis.
Citrullinated by PADI4.
Recycling endosome membrane>Lipid-anchor>Cytoplasmic side. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane>Lipid-anchor>Cytoplasmic side. Cytoplasmic vesicle>Phagosome membrane>Lipid-anchor>Cytoplasmic side.
Note: Recruited to phagosomes containing S.aureus.
Interacts with KCNMA1 (By similarity). Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. May interact with TBC1D14. Interacts with ATP6V1E1. Interacts with PI4KB. Interacts (GDP-bound form) with ZFYVE27. Interacts (GDP-bound form) with KIF5A in a ZFYVE27-dependent manner. Interacts with RELCH (By similarity). Interacts (in GTP-bound form) with TBC1D8B (via domain Rab-GAP TBC).
Belongs to the small GTPase superfamily. Rab family.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.
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