| 产品: | RAB3IP 抗体 |
| 货号: | AF9176 |
| 描述: | Rabbit polyclonal antibody to RAB3IP |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 53kDa; 53kD(Calculated). |
| 蛋白号: | Q96QF0 |
| RRID: | AB_2843366 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9176, RRID:AB_2843366.
展开/折叠
Gtpat12; Rab-3A-interacting protein; RAB3A interacting protein; RAB3A interacting protein (rabin3); RAB3A interacting protein; RAB3A-interacting protein; RAB3I_HUMAN; Rab3ip; Rabin 3; Rabin-3; RABIN3; RABIN3, rat, homolog of; Rabin8; SSX2 interacting protein; SSX2 interacting protein; SSX2-interacting protein;
抗原和靶标
Expressed in brain, kidney, heart, pancreas and placenta. Not detected in skeletal muscle or liver.
- Q96QF0 RAB3I_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGLKKMKGLSYDEAFAMANDPLEGFHEVNLASPTSPDLLGVYESGTQEQTTSPSVIYRPHPSALSSVPIQANALDVSELPTQPVYSSPRRLNCAEISSISFHVTDPAPCSTSGVTAGLTKLTTRKDNYNAEREFLQGATITEACDGSDDIFGLSTDSLSRLRSPSVLEVREKGYERLKEELAKAQRELKLKDEECERLSKVRDQLGQELEELTASLFEEAHKMVREANIKQATAEKQLKEAQGKIDVLQAEVAALKTLVLSSSPTSPTQEPLPGGKTPFKKGHTRNKSTSSAMSGSHQDLSVIQPIVKDCKEADLSLYNEFRLWKDEPTMDRTCPFLDKIYQEDIFPCLTFSKSELASAVLEAVENNTLSIEPVGLQPIRFVKASAVECGGPKKCALTGQSKSCKHRIKLGDSSNYYYISPFCRYRITSVCNFFTYIRYIQQGLVKQQDVDQMFWEVMQLRKEMSLAKLGYFKEEL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96QF0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y85 | Phosphorylation | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| T115 | Phosphorylation | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| T265 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| T268 | Phosphorylation | Uniprot | |
| T277 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Q9Y2H1 (STK38L) , Q15208 (STK38) | Uniprot |
| T289 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| T329 | Phosphorylation | Uniprot | |
| K383 | Acetylation | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| Y416 | Phosphorylation | Uniprot | |
| K462 | Methylation | Uniprot | |
| Y471 | Phosphorylation | Uniprot |
研究背景
Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis.
Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium.
Note: Predominantly cytoplasmic but a small proportion colocalizes with SSX2 in the nucleus. Activation of protein kinase C results in redistribution to the periphery of lamellipodia. In the cytoskeleton, localizes to cortical actin.
Expressed in brain, kidney, heart, pancreas and placenta. Not detected in skeletal muscle or liver.
Interacts with the N-terminal region of SSX2. Interacts with the GDP-bound forms of RAB8A and RAB8B. The interaction with RAB8A is prevented by phosphorylation of RAB8A at 'Thr-72'. Interacts with the GDP-bound forms of RAB3A and RAB3D. Interacts with DCDC1.
Belongs to the SEC2 family.
限制条款
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