Phospho-Histone H2A.X (Ser139)[Ser140] Antibody - #AF3187

Fig. 4 |TDP1 is the key target mediating the function of miR-211. a, c The shRNA technique effectively blocked the expression of TDP1 in HO8910 and SKOV3 cells. b, d Downregulation of TDP1 facilitated the sensitivity of HO8910 and SKOV3 cells to carboplatin. e Knockdown of TDP1 resulted in the upregulation of γ-H2AX in HO8910 and SKOV3 cells treated with carboplatin.

FIGURE 4 TOP2B knock down reduces various cellular aging hallmarks in human IMR-90 cells and in various mouse tissues. (A–H) IMR-90 cells were induced to undergo replicative, stress-induced, or oncogene-induced senescence. The protein levels of p16, p21, and γH2AX were detected by western blotting (A, B). γH2AX foci in DAPI-stained nuclei of IMR-90 cells were measured by immunofluorescent staining (C, D). The protein level of PARP1 was detected by western blotting (E, F). SA-β-Gal staining (blue-stained cells) and quantification of percent of SA-β-Gal positive cells were shown in G, H. (I–P) Confocal fluorescence images of IMR-90 cells (I–K) and C. elegans (N–P) stained by LysoTracker Red DND-99 (LTR) and LysoSensorTM Green DND-189 (LSG), and the fluorescence intensity ratio of LSG/LTR was measured as an indicator of lysosomal acidity. (L, M) IMR-90 cells were induced to undergo replicative, stress-induced, or oncogene-induced senescence. The protein level of TFEB was detected by western blotting. The relative intensity of LSG/LTR and average lysosome diameter in IMR-90 cells (J, K) and C. elegans (O, P) were quantified. (Q, R) Western blot analysis of nutrient-sensing mTOR signaling proteins in IMR-90 cells. (S–Y) Western blot analysis of nutrient-sensing mTOR signaling proteins in mouse kidney, lung, and skeletal muscle. Statistical analysis was performed using GraphPad Prism v8.0 software (https://www.graphpad.com). Data were considered statistically significant at p

Fig. 3. |Effect of DHI on oxidative stress and DNA injure in the ischemic penumbra.D-G: Representative images of WB analysis and the semi-quantification of SOD2, RhoGDI and γ-H2A.X. Data are expressed as mean ± SD (n = 3).

Fig. 3. |Effect of DHI on oxidative stress and DNA injure in the ischemic penumbra.H and I: Immunostaining photomicrographs of γ-H2A.X and quantitative analysis of the IOD. Bar = 50 µm. Data are expressed as mean ± SD (n = 4). *P < 0.05 versus CI/R group; **P < 0.01 versus CI/R group; ##P < 0.01 versus sham group.

Fig. 2. The condition of DNA damage in GC-1 cells and mouse testis. (A) Detection of DNA damage by comet assay and the statistics of OTM and TailDNA% in GC-1 cells. Bar= 50 µm. (B and C) The expression level of γ-H2AX protein in testicular tissues and GC-1 cells was detected with Western Blot. *p < 0.05 vs. the control group; #p < 0.05 vs. the corresponding MC-LR exposure group. All data were expressed as  ± SD (n = 3).

Fig. 7. Western blot detection of protein expression associated with ERS. (A) The protein expression of GRP78, PERK, IRE1, ATF6 and CHOP. (B-F) Statistical analysis of the protein expression (n = 3, mean ± SD. *p