| 产品: | Separase 抗体 |
| 货号: | AF9195 |
| 描述: | Rabbit polyclonal antibody to Separase |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Sheep, Rabbit, Dog |
| 分子量: | 230kDa; 233kD(Calculated). |
| 蛋白号: | Q14674 |
| RRID: | AB_2843385 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9195, RRID:AB_2843385.
展开/折叠
Caspase like protein ESPL1; Caspase-like protein ESPL1; ESP 1; ESP1; ESPL 1; Espl1; ESPL1_HUMAN; Extra spindle poles like 1; Extra spindle poles like 1 protein; Extra spindle poles-like 1 protein; FLJ46492; KIAA0165; Separase; Separin; Similar to fission yeast cut1and gene; SSE;
抗原和靶标
- Q14674 ESPL1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRSFKRVNFGTLLSSQKEAEELLPALKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKKLPSPCPDKESDKDLGPRLRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVRGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q14674 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| K491 | Ubiquitination | Uniprot | |
| K562 | Acetylation | Uniprot | |
| K562 | Ubiquitination | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| K593 | Ubiquitination | Uniprot | |
| Y719 | Phosphorylation | Uniprot | |
| K722 | Ubiquitination | Uniprot | |
| K755 | Ubiquitination | Uniprot | |
| K760 | Ubiquitination | Uniprot | |
| S810 | Phosphorylation | Uniprot | |
| Y973 | Phosphorylation | Uniprot | |
| K1008 | Ubiquitination | Uniprot | |
| K1016 | Ubiquitination | Uniprot | |
| K1020 | Ubiquitination | Uniprot | |
| S1073 | Phosphorylation | Uniprot | |
| K1083 | Ubiquitination | Uniprot | |
| T1111 | Phosphorylation | Uniprot | |
| S1122 | Phosphorylation | Uniprot | |
| T1123 | Phosphorylation | Uniprot | |
| S1126 | Phosphorylation | P06493 (CDK1) , P28482 (MAPK1) | Uniprot |
| S1153 | Phosphorylation | Uniprot | |
| K1189 | Ubiquitination | Uniprot | |
| T1210 | Phosphorylation | Uniprot | |
| K1252 | Ubiquitination | Uniprot | |
| S1305 | Phosphorylation | Uniprot | |
| K1309 | Ubiquitination | Uniprot | |
| S1316 | Phosphorylation | Uniprot | |
| K1322 | Ubiquitination | Uniprot | |
| S1325 | Phosphorylation | Uniprot | |
| K1336 | Ubiquitination | Uniprot | |
| R1341 | Methylation | Uniprot | |
| T1346 | Phosphorylation | Uniprot | |
| T1363 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S1396 | Phosphorylation | Uniprot | |
| S1399 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S1444 | Phosphorylation | Uniprot | |
| S1455 | Phosphorylation | Uniprot | |
| S1475 | Phosphorylation | Uniprot | |
| S1501 | Phosphorylation | P17612 (PRKACA) , Q9UQM7 (CAMK2A) | Uniprot |
| S1508 | Phosphorylation | Uniprot | |
| S1513 | Phosphorylation | Uniprot | |
| K1518 | Ubiquitination | Uniprot | |
| S1528 | Phosphorylation | Uniprot | |
| S1538 | Phosphorylation | Uniprot | |
| S1539 | Phosphorylation | Uniprot | |
| S1545 | Phosphorylation | Uniprot | |
| S1552 | Phosphorylation | Uniprot | |
| Y1614 | Phosphorylation | Uniprot | |
| S1649 | Phosphorylation | Uniprot | |
| S1660 | Phosphorylation | Uniprot | |
| K1692 | Ubiquitination | Uniprot | |
| K1732 | Ubiquitination | Uniprot | |
| K1747 | Ubiquitination | Uniprot | |
| S1752 | Phosphorylation | Uniprot | |
| K1762 | Ubiquitination | Uniprot | |
| S1768 | Phosphorylation | Uniprot | |
| S1769 | Phosphorylation | Uniprot | |
| T1771 | Phosphorylation | Uniprot | |
| K1796 | Ubiquitination | Uniprot | |
| K1803 | Ubiquitination | Uniprot | |
| S1820 | Phosphorylation | Uniprot | |
| K1832 | Ubiquitination | Uniprot | |
| T1884 | Phosphorylation | Uniprot | |
| S1887 | Phosphorylation | Uniprot | |
| S1889 | Phosphorylation | Uniprot | |
| K1901 | Ubiquitination | Uniprot | |
| K1994 | Ubiquitination | Uniprot | |
| K2103 | Ubiquitination | Uniprot | |
| Y2104 | Phosphorylation | Uniprot | |
| S2118 | Phosphorylation | Uniprot |
研究背景
Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
Autocleaves. This function, which is not essential for its protease activity, is unknown.
Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
Cytoplasm. Nucleus.
Interacts with PTTG1. Interacts with RAD21.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
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