产品: TRF1 抗体
货号: AF9217
描述: Rabbit polyclonal antibody to TRF1
应用: WB IF/ICC
反应: Human
预测: Horse, Sheep
分子量: 50kDa; 50kD(Calculated).
蛋白号: P54274
RRID: AB_2843407

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产品描述

来源:
Rabbit
应用:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Horse(83%), Sheep(83%)
克隆:
Polyclonal
特异性:
TRF1 Antibody detects endogenous levels of total TRF1.
RRID:
AB_2843407
引用格式: Affinity Biosciences Cat# AF9217, RRID:AB_2843407.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

hTRF1 AS; NIMA interacting protein 2; NIMA-interacting protein 2; PIN 2; PIN2; t TRF1; Telomeric protein Pin2; Telomeric protein Pin2/TRF1; Telomeric repeat binding factor (NIMA interacting) 1; Telomeric repeat binding factor 1; Telomeric repeat binding protein 1; Telomeric repeat-binding factor 1; TERF 1; Terf1; TERF1_HUMAN; TRBF 1; TRBF1; TRF 1; TRF; TTAGGG repeat binding factor 1; TTAGGG repeat-binding factor 1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P54274 TERF1_HUMAN:

Highly expressed and ubiquitous. Isoform Pin2 predominates.

序列:
MAEDVSSAAPSPRGCADGRDADPTEEQMAETERNDEEQFECQELLECQVQVGAPEEEEEEEEDAGLVAEAEAVAAGWMLDFLCLSLCRAFRDGRSEDFRRTRNSAEAIIHGLSSLTACQLRTIYICQFLTRIAAGKTLDAQFENDERITPLESALMIWGSIEKEHDKLHEEIQNLIKIQAIAVCMENGNFKEAEEVFERIFGDPNSHMPFKSKLLMIISQKDTFHSFFQHFSYNHMMEKIKSYVNYVLSEKSSTFLMKAAAKVVESKRTRTITSQDKPSGNDVEMETEANLDTRKSVSDKQSAVTESSEGTVSLLRSHKNLFLSKLQHGTQQQDLNKKERRVGTPQSTKKKKESRRATESRIPVSKSQPVTPEKHRARKRQAWLWEEDKNLRSGVRKYGEGNWSKILLHYKFNNRTSVMLKDRWRTMKKLKLISSDSED

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
83
Sheep
83
Bovine
75
Pig
67
Rabbit
67
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P54274 作为底物

Site PTM Type Enzyme
A2 Acetylation
S7 Phosphorylation
S11 Phosphorylation
S114 Phosphorylation Q8TDX7 (NEK7)
T122 Phosphorylation P68400 (CSNK2A1)
K136 Ubiquitination
T149 Phosphorylation
S160 Phosphorylation
K167 Ubiquitination
S219 Phosphorylation Q13315 (ATM)
K241 Acetylation
S252 Phosphorylation
S253 Phosphorylation
T254 Phosphorylation
S266 Phosphorylation
T271 Phosphorylation
T273 Phosphorylation P31749 (AKT1)
K295 Acetylation
S296 Phosphorylation
K300 Acetylation
K300 Ubiquitination
S308 Phosphorylation
T311 Phosphorylation
K319 Ubiquitination
K325 Ubiquitination
T330 Phosphorylation
T344 Phosphorylation P06493 (CDK1)
S347 Phosphorylation
T358 Phosphorylation
S367 Phosphorylation Q13315 (ATM)
T371 Phosphorylation P06493 (CDK1)
K397 Ubiquitination
K411 Ubiquitination
S434 Phosphorylation
S435 Phosphorylation P53350 (PLK1)
S437 Phosphorylation

研究背景

功能:

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.

翻译修饰:

Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.

ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.

Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.

细胞定位:

Nucleus. Cytoplasm>Cytoskeleton>Spindle. Chromosome>Telomere.
Note: Colocalizes with telomeric DNA in interphase and prophase cells. Telomeric localization decreases in metaphase, anaphase and telophase. Associates with the mitotic spindle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed and ubiquitous. Isoform Pin2 predominates.

亚基结构:

Homodimer; can contain both isoforms. Found in a complex with POT1; TINF2 and TNKS1. Interacts with ATM, TINF2, TNKS1, TNKS2, PINX1, NEK2 and MAPRE1. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP ACD and POT1. Interacts with RLIM (via N-terminus). Interacts with FBXO4. Interaction with TINF2 protects against interaction with FBXO4 and subsequent polyubiquitination and proteasomal degradation. Interacts with GNL3L; this interaction promotes homodimerization. Interacts with TIN2. Interacts with RTEL1. Interactions with GNL3L and TIN2 are mutually exclusive. Interacts with CCDC79/TERB1 (By similarity).

蛋白家族:

The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.

The TRFH dimerization region mediates the interaction with TINF2.

The HTH domain is an independent structural unit and mediates binding to telomeric DNA.

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