| 产品: | TRF1 抗体 |
| 货号: | AF9217 |
| 描述: | Rabbit polyclonal antibody to TRF1 |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Horse, Sheep |
| 分子量: | 50kDa; 50kD(Calculated). |
| 蛋白号: | P54274 |
| RRID: | AB_2843407 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9217, RRID:AB_2843407.
展开/折叠
hTRF1 AS; NIMA interacting protein 2; NIMA-interacting protein 2; PIN 2; PIN2; t TRF1; Telomeric protein Pin2; Telomeric protein Pin2/TRF1; Telomeric repeat binding factor (NIMA interacting) 1; Telomeric repeat binding factor 1; Telomeric repeat binding protein 1; Telomeric repeat-binding factor 1; TERF 1; Terf1; TERF1_HUMAN; TRBF 1; TRBF1; TRF 1; TRF; TTAGGG repeat binding factor 1; TTAGGG repeat-binding factor 1;
抗原和靶标
- P54274 TERF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEDVSSAAPSPRGCADGRDADPTEEQMAETERNDEEQFECQELLECQVQVGAPEEEEEEEEDAGLVAEAEAVAAGWMLDFLCLSLCRAFRDGRSEDFRRTRNSAEAIIHGLSSLTACQLRTIYICQFLTRIAAGKTLDAQFENDERITPLESALMIWGSIEKEHDKLHEEIQNLIKIQAIAVCMENGNFKEAEEVFERIFGDPNSHMPFKSKLLMIISQKDTFHSFFQHFSYNHMMEKIKSYVNYVLSEKSSTFLMKAAAKVVESKRTRTITSQDKPSGNDVEMETEANLDTRKSVSDKQSAVTESSEGTVSLLRSHKNLFLSKLQHGTQQQDLNKKERRVGTPQSTKKKKESRRATESRIPVSKSQPVTPEKHRARKRQAWLWEEDKNLRSGVRKYGEGNWSKILLHYKFNNRTSVMLKDRWRTMKKLKLISSDSED
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P54274 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Q8TDX7 (NEK7) | Uniprot |
| T122 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K136 | Ubiquitination | Uniprot | |
| T149 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| S219 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K241 | Acetylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| T271 | Phosphorylation | Uniprot | |
| T273 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K295 | Acetylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| K300 | Acetylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| K319 | Ubiquitination | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| T330 | Phosphorylation | Uniprot | |
| T344 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S347 | Phosphorylation | Uniprot | |
| T358 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Q13315 (ATM) | Uniprot |
| T371 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K397 | Ubiquitination | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S437 | Phosphorylation | Uniprot |
研究背景
Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
Nucleus. Cytoplasm>Cytoskeleton>Spindle. Chromosome>Telomere.
Note: Colocalizes with telomeric DNA in interphase and prophase cells. Telomeric localization decreases in metaphase, anaphase and telophase. Associates with the mitotic spindle.
Highly expressed and ubiquitous. Isoform Pin2 predominates.
Homodimer; can contain both isoforms. Found in a complex with POT1; TINF2 and TNKS1. Interacts with ATM, TINF2, TNKS1, TNKS2, PINX1, NEK2 and MAPRE1. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP ACD and POT1. Interacts with RLIM (via N-terminus). Interacts with FBXO4. Interaction with TINF2 protects against interaction with FBXO4 and subsequent polyubiquitination and proteasomal degradation. Interacts with GNL3L; this interaction promotes homodimerization. Interacts with TIN2. Interacts with RTEL1. Interactions with GNL3L and TIN2 are mutually exclusive. Interacts with CCDC79/TERB1 (By similarity).
The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
The TRFH dimerization region mediates the interaction with TINF2.
The HTH domain is an independent structural unit and mediates binding to telomeric DNA.
限制条款
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