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| 产品: | V-ATPase H 抗体 |
| 货号: | AF9227 |
| 描述: | Rabbit polyclonal antibody to V-ATPase H |
| 应用: | WB IHC |
| 反应: | Human, Mouse |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 55kDa; 56kD(Calculated). |
| 蛋白号: | Q9UI12 |
| RRID: | AB_2843417 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9227, RRID:AB_2843417.
展开/折叠
Atp6v1h; ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H; CGI 11; CGI11; EC 3.6.3.14; MSTP042; NBP1; Nef binding protein 1; Nef-binding protein 1; Protein VMA13 homolog; SFD; SFDalpha; SFDbeta; V ATPase subunit H; V-ATPase 50/57 kDa subunits; V-ATPase subunit H; V-type proton ATPase subunit H; Vacuolar ATP synthase subunit H; Vacuolar proton pump subunit H; Vacuolar proton pump subunit SFD; VATH_HUMAN; VMA13;
抗原和靶标
- Q9UI12 VATH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTKMDIRGAVDAAVPTNIIAAKAAEVRANKVNWQSYLQGQMISAEDCEFIQRFEMKRSPEEKQEMLQTEGSQCAKTFINLMTHICKEQTVQYILTMVDDMLQENHQRVSIFFDYARCSKNTAWPYFLPMLNRQDPFTVHMAARIIAKLAAWGKELMEGSDLNYYFNWIKTQLSSQKLRGSGVAVETGTVSSSDSSQYVQCVAGCLQLMLRVNEYRFAWVEADGVNCIMGVLSNKCGFQLQYQMIFSIWLLAFSPQMCEHLRRYNIIPVLSDILQESVKEKVTRIILAAFRNFLEKSTERETRQEYALAMIQCKVLKQLENLEQQKYDDEDISEDIKFLLEKLGESVQDLSSFDEYSSELKSGRLEWSPVHKSEKFWRENAVRLNEKNYELLKILTKLLEVSDDPQVLAVAAHDVGEYVRHYPRGKRVIEQLGGKQLVMNHMHHEDQQVRYNALLAVQKLMVHNWEYLGKQLQSEQPQTAAARS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UI12 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T16 | Phosphorylation | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| K371 | Ubiquitination | Uniprot | |
| K374 | Ubiquitination | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| Y388 | Phosphorylation | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| Y466 | Phosphorylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| S483 | Phosphorylation | Uniprot |
研究背景
Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes.
Widely expressed.
V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). Interacts with AP2M1. Interacts with TM9SF4 in colon cancer cells.
(Microbial infection) Interacts with HIV-1 Nef protein.
Belongs to the V-ATPase H subunit family.
研究领域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Immune diseases > Rheumatoid arthritis.
· Metabolism > Energy metabolism > Oxidative phosphorylation.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Nervous system > Synaptic vesicle cycle.
文献引用
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