MMP1 Antibody | Affinity Biosciences LTD|亲科生物官网

WB
IF/ICC
Cites

1/8

Western blot analysis of extracts from Hela, using MMP1 Antibody.

AF0209 staining HepG2 cells by IF/ICC.

Fig.

Figure 9 Representative immunohistochemical staining with MMP1, MMP3, MMP7, MMP9 and MMP12 in colon tissues in mice treated with DSS.

FIGURE 2 |e) Western blot analysis (right panel) and band intensity analysis (left panel) of the protein levels of Survivin, Mmp1, and Mmp13 in Control+Vehicle, Vector+IL-1β, and oeUSP49 +IL-1β rat chondro-cytes.

Figure 5.

Figure 4 FA promoted collagen metabolism.

Figure 4.

产品:	MMP1 抗体
货号:	AF0209
描述:	Rabbit polyclonal antibody to MMP1
应用:	WB IF/ICC
反应:	Human
预测:	Pig, Bovine, Horse, Sheep, Rabbit
分子量:	54kDa; 54kD(Calculated).
蛋白号:	P03956
RRID:	AB_2833396

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human

预测:

Pig(88%), Bovine(88%), Horse(100%), Sheep(94%), Rabbit(100%)

克隆:

Polyclonal

特异性:

MMP1 Antibody detects endogenous levels of total MMP1.

RRID:

AB_2833396
引用格式: Affinity Biosciences Cat# AF0209, RRID:AB_2833396.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

27 kDa interstitial collagenase; CLG; CLGN; collagenase, fibroblast; collagenase, interstitial; Fibroblast collagenase; Interstitial collagenase; Matrix metallopeptidase 1 (interstitial collagenase); Matrix metalloprotease 1; Matrix Metalloproteinase 1; Matrix metalloproteinase-1; MMP 1; MMP-1; MMP1; MMP1_HUMAN; OTTHUMP00000045866;

抗原和靶标

免疫原:

Uniprot:

P03956(MMP1_HUMAN) >>Visit HPA database.

基因/基因ID:

MMP1(4312)

描述:

MMP1 Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Belongs to the peptidase M10A family.

序列:

P03956 MMP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MHSFPPLLLLLFWGVVSHSFPATLETQEQDVDLVQKYLEKYYNLKNDGRQVEKRRNSGPVVEKLKQMQEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDRFYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGYPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFPGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Rabbit

100

Horse

100

Sheep

Pig

Bovine

Dog

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P03956 作为底物

P03956

Site	PTM Type	Source
S57	Phosphorylation	Uniprot
Y121	Phosphorylation	Uniprot
T122	Phosphorylation	Uniprot
S142	Phosphorylation	Uniprot
S153	Phosphorylation	Uniprot
T274	Phosphorylation	Uniprot
S280	Phosphorylation	Uniprot
T288	Phosphorylation	Uniprot
T289	Phosphorylation	Uniprot
Y360	Phosphorylation	Uniprot
K450	Ubiquitination	Uniprot

研究背景

P03956_MMP1_HUMAN

功能:

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.

翻译修饰:

Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.

Tyrosine phosphorylated in platelets by PKDCC/VLK.

细胞定位:

Secreted>Extracellular space>Extracellular matrix.

亚基结构:

(Microbial infection) Interacts with HIV-1 Tat.

蛋白家族:

There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

研究领域

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer. (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

文献引用

1). Parthenolide attenuated bleomycin-induced pulmonary fibrosis via the NF-κB/Snail signaling pathway. RESPIRATORY RESEARCH, 2018 (PubMed: 29871641) [IF=5.8]

Application: IHC Species: human Sample: lung

Fig. 6| PTL attenuates the BLM-induced expression of EMT-related protein. a Representative immunohistochemical staining of lung sections showing cadherin, vimentin, MMP1, α-SMA and Col-1 staining.

2). Timosaponin B-II alleviates osteoarthritis-related inflammation and extracellular matrix degradation through inhibition of mitogen-activated protein kinases and nuclear factor-κB pathways in vitro. Bioengineered, 2022 (PubMed: 35094658) [IF=4.9]

Application: WB Species: Rat Sample: SW1353 cells and chondrocytes

Figure 4. TB-II inhibited the production of cartilage degrading enzymes in IL-1β-treated SW1353 cells and chondrocytes. The mRNA expression of (a) MMP-1, (b) MMP-3 and (c) MMP-13 were detected by qRT-PCR. (d-g) The protein level of MMP-1, MMP-3, and MMP-13 were detected by Western blot. GAPDH was conducted as a loading control. One-way ANOVA, ##p < 0.01, compared with control cells; $p < 0.05, $$p < 0.01, ns: not-significant, compared with IL-1β-treated cells.

3). Identification of Active Compounds and Mechanism of Huangtu Decoction for the Treatment of Ulcerative Colitis by Network Pharmacology Combined with Experimental Verification. Drug Design Development and Therapy, 2021 (PubMed: 34616145) [IF=4.8]

Application: IHC Species: Mice Sample: colon tissues

Figure 9 Representative immunohistochemical staining with MMP1, MMP3, MMP7, MMP9 and MMP12 in colon tissues in mice treated with DSS. (A) Immunohistochemical staining for MMP1, MMP3, MMP7, MMP9 and MMP12 in normal, model and DSS+HTD group. (B) Statistical analysis of relative immunohistochemical staining intensity for MMP1, MMP3, MMP7, MMP9 and MMP12 in normal, model and DSS+HTD group.*P < 0.05, **P < 0.01 and ***P < 0.001 versus model group.

4). Ubiquitin-specific protease 49 attenuates IL-1β-induced rat primary chondrocyte apoptosis by facilitating Axin deubiquitination and subsequent Wnt/β-catenin signaling cascade inhibition. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2020 (PubMed: 32737772) [IF=4.3]

Application: WB Species: rat Sample: chondrocytes

FIGURE 2 |e) Western blot analysis (right panel) and band intensity analysis (left panel) of the protein levels of Survivin, Mmp1, and Mmp13 in Control+Vehicle, Vector+IL-1β, and oeUSP49 +IL-1β rat chondro-cytes. ***P<0.001 versus the Vector or Control+Vehicle group; ## and ### indicate P values less than 0.01 and 0.001,respectively, versus the Vec-tor+IL-1β group

5). Pathological changes and expression of lysine oxidases and matrix metalloproteinases‐1,‐2, and‐3 in ligaments of patients with haemophilic arthritis. HAEMOPHILIA, 2022 (PubMed: 34697874) [IF=3.9]

Application: WB Species: human Sample: cruciate ligaments

Figure 5.|LOXs and MMP-1,-2,and -3protein expression levels in the cruciate ligaments of patients with OA or HA

6). Circ-Sirt1 inhibits proliferation, induces apoptosis, and ameliorates inflammation in human rheumatoid arthritis fibroblast-like synoviocytes. AUTOIMMUNITY, 2021 (PubMed: 34431434) [IF=3.5]

7). Forsythiaside A Regulates Activation of Hepatic Stellate Cells by Inhibiting NOX4-Dependent ROS. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 35035671)

Application: WB Species: Rat Sample: HSCs

Figure 4 FA promoted collagen metabolism. (A-B) The expression of MMP-1 and TIMP-1 mRNA. (C-D) The expression of MMP-1 and TIMP-1 proteins. (E-G) The content of MMP-1 and TIMP-1 in medium supernatant. (H) The content of hydroxyproline. Data of three independent experiments were represented by mean ± S.D. ∗∗∗p <0.001 TGF-β1 vs control; ∗∗p <0.01 TGF-β1 vs control. ###p <0.001 TGF-β1 + FA vs TGF-β1;##p <0.01 TGF-β1 + FA vs TGF-β1; #p <0.05 TGF-β1 + FA vs TGF-β1.

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MMP1 Antibody - #AF0209

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