产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9229, RRID:AB_2843419.
展开/折叠
;WASP family, verprolin homology domain-containing protein 1; WASP family member 1; FLJ31482; homology of dictyostelium scar 1; KIAA0269; Protein WAVE-1; Protein WAVE1; scar, Dictyostelium, homology of, 1; SCAR1; Similar to a plant extensin like protein; Verprolin homology domain containing protein 1; Verprolin homology domain-containing protein 1; WAS protein family, member 1; Wasf1; WASF1_HUMAN; WASL; WASP family 1; WASP family protein member 1; WASP family, verprolin homology domain-containing protein; WAVE; WAVE1; Wiskott Aldrich syndrome protein family member 1; Wiskott-Aldrich syndrome protein family member 1;
抗原和靶标
Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.
- Q92558 WASF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNILTPYRDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQGPELAEDDANLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLTRAEERVLVRPHEPPPPPPMHGAGDAKPIPTCISSATGLIENRPQSPATGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPPPVPPPPPPPATALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEVQGLPPPPPPPPLPPPGIRPSSPVTVTALAHPPSGLHPTPSTAPGPHVPLMPPSPPSQVIPASEPKRHPSTLPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q92558 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S91 | Phosphorylation | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| Y125 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y151 | Phosphorylation | Uniprot | |
| T169 | Phosphorylation | Uniprot | |
| K182 | Acetylation | Uniprot | |
| K192 | Acetylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| R307 | Methylation | Uniprot | |
| S310 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| R315 | Methylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| T323 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| S397 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| S441 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| S489 | Phosphorylation | Uniprot | |
| T490 | Phosphorylation | Uniprot | |
| Y543 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| S550 | Phosphorylation | Uniprot |
研究背景
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity).
Phosphorylated on tyrosine residues by ABL1 and dephosphorylated by PTPN12.
Cytoplasm>Cytoskeleton. Cell junction>Synapse. Cell junction>Focal adhesion.
Note: Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas (PubMed:9889097). Partial translocation to focal adhesion sites might be mediated by interaction with SORBS2 (PubMed:18559503). In neurons, colocalizes with activated NTRK2 after BDNF addition in endocytic sites through the association with TMEM108 (By similarity).
Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.
Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Interacts with SHANK3; the interaction mediates the association of SHANK3 with the WAVE1 complex. Interacts with ABI1 (via N-terminus). Interacts with SORBS2; this interaction greatly enhances phosphorylation by ABL1 and dephosphorylation by PTPN12 and might mediate partial to focal adhesion sites.
Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.
Belongs to the SCAR/WAVE family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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