SLC1A5 Antibody - #AF6610

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产品: SLC1A5 抗体
货号: AF6610
描述: Rabbit polyclonal antibody to SLC1A5
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 56 kDa; 57kD(Calculated).
蛋白号: Q15758
RRID: AB_2843431

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WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
SLC1A5 Antibody detects endogenous levels of total SLC1A5.
RRID:
AB_2843431
引用格式: Affinity Biosciences Cat# AF6610, RRID:AB_2843431.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ASCT2; AAAT; AAAT_HUMAN; ATB(0); ATBO; Baboon M7 virus receptor; FLJ31068; M7V1; M7VS1; Neutral amino acid transporter B(0); R16; RD114/simian type D retrovirus receptor; RDR; RDRC; SLC1A5; Sodium dependent neutral amino acid transporter type 2; Sodium-dependent neutral amino acid transporter type 2; Solute carrier family 1 (neutral amino acid transporter), member 5; Solute carrier family 1 member 5;

抗原和靶标

免疫原:
Uniprot:

Q15758(AAAT_HUMAN) >>Visit HPA database.

基因/基因ID:
SLC1A5(6510)
表达:
Q15758 AAAT_HUMAN:

Placenta, lung, skeletal muscle, kidney, pancreas, and intestine.

描述:
Sodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids. May also be activated by insulin. Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development (PubMed:10708449, PubMed:23492904). Acts as a cell surface receptor for feline endogenous virus RD114, baboon M7 endogenous virus and type D simian retroviruses (PubMed:10051606, PubMed:10196349).
序列:
MVADPPRDSKGLAAAEPTANGGLALASIEDQGAAAGGYCGSRDQVRRCLRANLLVLLTVVAVVAGVALGLGVSGAGGALALGPERLSAFVFPGELLLRLLRMIILPLVVCSLIGGAASLDPGALGRLGAWALLFFLVTTLLASALGVGLALALQPGAASAAINASVGAAGSAENAPSKEVLDSFLDLARNIFPSNLVSAAFRSYSTTYEERNITGTRVKVPVGQEVEGMNILGLVVFAIVFGVALRKLGPEGELLIRFFNSFNEATMVLVSWIMWYAPVGIMFLVAGKIVEMEDVGLLFARLGKYILCCLLGHAIHGLLVLPLIYFLFTRKNPYRFLWGIVTPLATAFGTSSSSATLPLMMKCVEENNGVAKHISRFILPIGATVNMDGAALFQCVAAVFIAQLSQQSLDFVKIITILVTATASSVGAAGIPAGGVLTLAIILEAVNLPVDHISLILAVDWLVDRSCTVLNVEGDALGAGLLQNYVDRTESRSTEPELIQVKSELPLDPLPVPTEEGNPLLKHYRGPAGDATVASEKESVM

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
91
Zebrafish
89
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15758 作为底物

Site PTM Type Enzyme
M1 Acetylation
S9 Phosphorylation
K10 Ubiquitination
S27 Phosphorylation
Y38 Phosphorylation
K178 Ubiquitination
S183 Phosphorylation
S194 Phosphorylation
S198 Phosphorylation
N212 N-Glycosylation
K247 Ubiquitination
K372 Ubiquitination
S493 Phosphorylation
T494 Phosphorylation
K502 Ubiquitination
S503 Phosphorylation
K522 Sumoylation
K522 Ubiquitination
Y524 Phosphorylation
R525 Methylation
T532 Phosphorylation
S535 Phosphorylation
K537 Acetylation
K537 Ubiquitination
S539 Phosphorylation

研究背景

功能:

Sodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids. Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development.

(Microbial infection) Acts as a cell surface receptor for Feline endogenous virus RD114.

(Microbial infection) Acts as a cell surface receptor for Baboon M7 endogenous virus.

(Microbial infection) Acts as a cell surface receptor for type D simian retroviruses.

细胞定位:

Cell membrane>Multi-pass membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Placenta, lung, skeletal muscle, kidney, pancreas, and intestine.

亚基结构:

Homotrimer (Probable). Interacts with ERVH48-1/suppressyn; may negatively regulate syncytialization.

蛋白家族:

Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A5 subfamily.

研究领域

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Organismal Systems > Digestive system > Protein digestion and absorption.

文献引用

1). Tripartite motif containing 33 demonstrated anticancer effect by degrading c‑Myc: Limitation of glutamine metabolism and proliferation in endometrial carcinoma cells. International journal of oncology, 2023 (PubMed: 37859625) [IF=5.2]

Application: WB    Species: Human    Sample: EC cell

Figure 4 TRIM33 overexpression inhibits the glutamine metabolism in HEC-1-A and AN3CA cells in vitro. (A) Glutamine uptake and (B) intracellular glutamate production of TRIM33-silenced or -overexpressed EC cells were analyzed using the commercial kits. (C and D) Reverse transcription-quantitative PCR and western blotting were used to detect the expression of SLC1A5 and GLS in EC cells. *P
2). Babaodan overcomes cisplatin resistance in cholangiocarcinoma via inhibiting YAP1. Pharmaceutical biology, 2024 (PubMed: 38571483) [IF=3.8]

Application: WB    Species: Human    Sample: CCAs

Figure 6. The extent of apoptosis, glutathione (GSH) synthesis, and the expression of DNA damage-related proteins were assessed in cholangiocarcinoma cells (CCAs) subjected to YAP1 knockdown or YAP1 overexpression. Western blot was used to measure protein levels (n = 3). With cisplatin incubation, YAP1 knockdown and 1 mg/mL babaodan (BBD) treatment decreased the (a) Bcl-2 level and (b) increased bax level, while the change in (c) cle-caspase-3/caspase-3 levels with BBD treatment was not statistically significant. In the CCAs dealing with cisplatin, the expression levels of (d) p-YAP1/YAP1, (e) ATF4, and (f) SLC1A5 were decreased by YAP1 knockdown and BBD treatment. Additionally, the (g) γH2Ax level was increased and (h) the ERCC1 level was inhibited by YAP1 knockdown and BBD treatment. YAP1 overexpression antagonized the effect of BBD on these proteins. Representative protein bands are shown in (i), (j), and (k). (mean ± standard deviation) +p 
3). Dysfunction of Cytotoxic T Lymphocyte Induced by Hepatoma Cells through the Gln-GLS2-Endoplasmic Reticulum Stress Pathway. Frontiers in Bioscience-Landmark, 2022 (PubMed: 36042180)

Application: WB    Species: Mouse    Sample: cytotoxic T lymphocytes (CTLs) and hepatoma cells

Fig. 4. Glutamine (Gln) utilization in cytotoxic T lymphocytes (CTLs) and hepatoma cells. (A) Western blots were used to detect the protein expression levels of GLS1, GLS2, and SLC1A5 in the different groups. (B) After Gln deprivation, protein expression levels of GLS1, GLS2, and SLC1A5 in both CTLs and hepatoma cells were assessed by western blots.

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