MeCP2 Antibody - #AF9265

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产品: MeCP2 抗体
货号: AF9265
描述: Rabbit polyclonal antibody to MeCP2
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Horse, Rabbit, Dog
分子量: 53 kDa.; 52kD(Calculated).
蛋白号: P51608
RRID: AB_2843443

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Horse(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
MeCP2 Antibody detects endogenous levels of total MeCP2.
RRID:
AB_2843443
引用格式: Affinity Biosciences Cat# AF9265, RRID:AB_2843443.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AUTSX 3; AUTSX3; DKFZp686A24160; Mbd 5; Mbd5; MECP 2; MeCP 2 protein; MeCP-2 protein; Mecp2; MECP2_HUMAN; Methyl CpG binding protein 2 (Rett syndrome); Methyl CpG binding protein 2; Methyl-CpG-binding protein 2; MRX 16; MRX 79; MRX16; MRX79; MRXS 13; MRXS13; MRXSL; PPMX; RS; RTS; RTT; WBP 10; WBP10;

抗原和靶标

免疫原:
Uniprot:

P51608(MECP2_HUMAN) >>Visit HPA database.

基因/基因ID:
MECP2(4204)
表达:
P51608 MECP2_HUMAN:

Present in all adult somatic tissues tested.

描述:
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
序列:
MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
75
Sheep
67
Bovine
62
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P51608 作为底物

Site PTM Type Enzyme
S13 Phosphorylation
S53 Phosphorylation
S70 Phosphorylation
S78 Phosphorylation
S80 Phosphorylation Q9H2X6 (HIPK2)
S86 Phosphorylation
R115 Methylation
S116 Phosphorylation
K119 Methylation
Y120 Phosphorylation
K130 Ubiquitination
S149 Phosphorylation
T158 Phosphorylation
R162 Methylation
S164 Phosphorylation
S166 Phosphorylation
S178 Phosphorylation
T184 Phosphorylation
T203 Phosphorylation
S204 Phosphorylation
K210 Methylation
S216 Phosphorylation
K219 Acetylation
K223 Ubiquitination
T228 Phosphorylation
S229 Phosphorylation
K267 Methylation
S274 Phosphorylation
K285 Acetylation
T308 Phosphorylation
T311 Phosphorylation
S313 Phosphorylation
K321 Acetylation
T327 Phosphorylation
S332 Phosphorylation
K347 Methylation
S349 Phosphorylation
S355 Phosphorylation
S357 Phosphorylation
S359 Phosphorylation
S360 Phosphorylation
S395 Phosphorylation
S423 Phosphorylation
S426 Phosphorylation
K449 Acetylation
T479 Phosphorylation
S486 Phosphorylation

研究背景

功能:

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).

翻译修饰:

Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.

细胞定位:

Nucleus.
Note: Colocalized with methyl-CpG in the genome. Colocalized with TBL1X to the heterochromatin foci.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Present in all adult somatic tissues tested.

亚基结构:

Interacts with FNBP3 (By similarity). Interacts with CDKL5. Interacts with ATRX; MECP2 recruits ATRX to pericentric heterochromatin in neuronal cells (By similarity). Interacts with NCOR2 (By similarity). Interacts with TBL1XR1; bridges interaction between MECP2 and NCOR1. Interacts with TBL1X; recruits TBL1X to the heterochromatin foci.

文献引用

1). The role of BDNF transcription in the antidepressant-like effects of 18β-glycyrrhetinic acid in a chronic social defeat stress model. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38851983) [IF=7.9]
2). Inhibitory effect of bound polyphenol from foxtail millet bran on miR-149 methylation increases the chemosensitivity of human colorectal cancer HCT-8/Fu cells. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2021 (PubMed: 33011952) [IF=4.3]

Application: WB    Species: human    Sample: HCT-8 and HCT-8/Fu cells

Fig. 4 |Efects of BPIS on the expression of DNA methylation-related enzymes. a, b The expressions of DNMT3a, DNMT3b and MECP2 were measured in RNA and protein levels in HCT-8 and HCT-8/Fu cells by RT-PCR and western blotting assays.
3). CircMAPK1 promotes the proliferation and migration of vascular smooth muscle cells through miR-22-3p/methyl-CpG binding protein 2 axis. Nutrition, metabolism, and cardiovascular diseases : NMCD, 2021 (PubMed: 34059381) [IF=3.9]

Application: IF/ICC    Species: mouse    Sample:

Figure 4| CircMAPK1 can bind with and regulate miR-22e3p.The protein level of MECP2 was detected by Western blot (H) After indicated treatment,the expression and distribution of MECP2 was detected by immunofluorescence. Scale bar Z 50 mm. Each experiment was repeated for three times and the results were presented as mean SD. ))p < 0.01, )))p < 0.001. MECP2, methyl-CpG binding protein 2.

Application: WB    Species: mouse    Sample:

Figure 4| CircMAPK1 can bind with and regulate miR-22e3p.(E) The mRNA level of MECP2 was detected by quantitative real-time PCR (FeG)

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