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Western blot analysis of ketohexokinase expression in Mouse kidney lysate.

Western blot analysis of extracts from Mouse kidney, using ketohexokinase Antibody.

产品:	ketohexokinase 抗体
货号:	AF7029
描述:	Rabbit polyclonal antibody to ketohexokinase
应用:	WB
反应:	Human, Mouse
预测:	Bovine, Horse, Sheep
分子量:	33 KD; 33kD(Calculated).
蛋白号:	P50053
RRID:	AB_2843469

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

联系销售

MSDS

说明书

COA

实验方案

WB Handbook

IHC Protocol

IF/ICC Protocol

产品描述

来源:

Rabbit

应用:

WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse

预测:

Bovine(88%), Horse(100%), Sheep(88%)

克隆:

Polyclonal

特异性:

ketohexokinase Antibody detects endogenous levels of total ketohexokinase.

RRID:

AB_2843469
引用格式: Affinity Biosciences Cat# AF7029, RRID:AB_2843469.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

EC 2.7.1.3; Fructokinase; Hepatic fructokinase; Ketohexokinase; Ketohexokinase isoform a; KHK; KHK_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human ketohexokinase, corresponding to a region within N-terminal amino acids.

Uniprot:

P50053(KHK_HUMAN) >>Visit HPA database.

基因/基因ID:

KHK(3795)

表达:

P50053 KHK_HUMAN:

Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.

描述:

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

序列:

P50053 KHK_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEEKQILCVGLVVLDVISLVDKYPKEDSEIRCLSQRWQRGGNASNSCTVLSLLGAPCAFMGSMAPGHVADFLVADFRRRGVDVSQVAWQSKGDTPSSCCIINNSNGNRTIVLHDTSLPDVSATDFEKVDLTQFKWIHIEGRNASEQVKMLQRIDAHNTRQPPEQKIRVSVEVEKPREELFQLFGYGDVVFVSKDVAKHLGFQSAEEALRGLYGRVRKGAVLVCAWAEEGADALGPDGKLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSQGRSVQEALRFGCQVAGKKCGLQGFDGIV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Horse

100

Bovine

Sheep

Pig

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P50053 作为底物

P50053

Site	PTM Type	Source
S90	Phosphorylation	Uniprot
S116	Phosphorylation	Uniprot
K134	Ubiquitination	Uniprot
K148	Ubiquitination	Uniprot
K197	Ubiquitination	Uniprot

研究背景

P50053_KHK_HUMAN

功能:

Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.

组织特异性:

Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.

亚基结构:

Homodimer.

蛋白家族:

Belongs to the carbohydrate kinase PfkB family.

研究领域

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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ketohexokinase Antibody - #AF7029