产品: 磷酸化 SETD2 (Ser532) 抗体
货号: AF7052
描述: Rabbit polyclonal antibody to Phospho-SETD2 (Ser532)
应用: WB IHC
反应: Human, Mouse, Rat
分子量: 288kDa; 288kD(Calculated).
蛋白号: Q9BYW2
RRID: AB_2843492

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Phospho-SETD2 (Ser532) Antibody detects endogenous levels of SETD2 only when phosphorylated at Ser532.
RRID:
AB_2843492
引用格式: Affinity Biosciences Cat# AF7052, RRID:AB_2843492.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EC 2.1.1.43; FLJ16420; FLJ22472; FLJ23184; FLJ45883; HBP231; HIF 1; HIF-1; HIF1; HIP-1; Histone lysine N methyltransferase SETD2; Histone-lysine N-methyltransferase SETD2; hSET2; HSPC069; Huntingtin interacting protein 1; Huntingtin interacting protein; Huntingtin interacting protein B; Huntingtin interacting protein HYPB; Huntingtin yeast partner B; Huntingtin-binding protein, 231-KD; Huntingtin-interacting protein 1; Huntingtin-interacting protein B; HYPB; KIAA1732; KMT3A; Lysine N methyltransferase 3A; Lysine N-methyltransferase 3A; p231HBP; SET domain containing 2; SET domain-containing protein 2; SET2; SETD2; SETD2_HUMAN;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q9BYW2 SETD2_HUMAN:

Ubiquitously expressed.

序列:
MKQLQPQPPPKMGDFYDPEHPTPEEEENEAKIENVQKTGFIKGPMFKGVASSRFLPKGTKTKVNLEEQGRQKVSFSFSLTKKTLQNRFLTALGNEKQSDTPNPPAVPLQVDSTPKMKMEIGDTLSTAEESSPPKSRVELGKIHFKKHLLHVTSRPLLATTTAVASPPTHAAPLPAVIAESTTVDSPPSSPPPPPPPAQATTLSSPAPVTEPVALPHTPITVLMAAPVPLPVDVAVRSLKEPPIIIVPESLEADTKQDTISNSLEEHVTQILNEQADISSKKEDSHIGKDEEIPDSSKISLSCKKTGSKKKSSQSEGIFLGSESDEDSVRTSSSQRSHDLKFSASIEKERDFKKSSAPLKSEDLGKPSRSKTDRDDKYFSYSKLERDTRYVSSRCRSERERRRSRSHSRSERGSRTNLSYSRSERSHYYDSDRRYHRSSPYRERTRYSRPYTDNRARESSDSEEEYKKTYSRRTSSHSSSYRDLRTSSYSKSDRDCKTETSYLEMERRGKYSSKLERESKRTSENEAIKRCCSPPNELGFRRGSSYSKHDSSASRYKSTLSKPIPKSDKFKNSFCCTELNEEIKQSHSFSLQTPCSKGSELRMINKNPEREKAGSPAPSNRLNDSPTLKKLDELPIFKSEFITHDSHDSIKELDSLSKVKNDQLRSFCPIELNINGSPGAESDLATFCTSKTDAVLMTSDDSVTGSELSPLVKACMLSSNGFQNISRCKEKDLDDTCMLHKKSESPFRETEPLVSPHQDKLMSMPVMTVDYSKTVVKEPVDTRVSCCKTKDSDIYCTLNDSNPSLCNSEAENIEPSVMKISSNSFMNVHLESKPVICDSRNLTDHSKFACEEYKQSIGSTSSASVNHFDDLYQPIGSSGIASSLQSLPPGIKVDSLTLLKCGENTSPVLDAVLKSKKSSEFLKHAGKETIVEVGSDLPDSGKGFASRENRRNNGLSGKCLQEAQEEGNSILPERRGRPEISLDERGEGGHVHTSDDSEVVFSSCDLNLTMEDSDGVTYALKCDSSGHAPEIVSTVHEDYSGSSESSNDESDSEDTDSDDSSIPRNRLQSVVVVPKNSTLPMEETSPCSSRSSQSYRHYSDHWEDERLESRRHLYEEKFESIASKACPQTDKFFLHKGTEKNPEISFTQSSRKQIDNRLPELSHPQSDGVDSTSHTDVKSDPLGHPNSEETVKAKIPSRQQEELPIYSSDFEDVPNKSWQQTTFQNRPDSRLGKTELSFSSSCEIPHVDGLHSSEELRNLGWDFSQEKPSTTYQQPDSSYGACGGHKYQQNAEQYGGTRDYWQGNGYWDPRSGRPPGTGVVYDRTQGQVPDSLTDDREEEENWDQQDGSHFSDQSDKFLLSLQKDKGSVQAPEISSNSIKDTLAVNEKKDFSKNLEKNDIKDRGPLKKRRQEIESDSESDGELQDRKKVRVEVEQGETSVPPGSALVGPSCVMDDFRDPQRWKECAKQGKMPCYFDLIEENVYLTERKKNKSHRDIKRMQCECTPLSKDERAQGEIACGEDCLNRLLMIECSSRCPNGDYCSNRRFQRKQHADVEVILTEKKGWGLRAAKDLPSNTFVLEYCGEVLDHKEFKARVKEYARNKNIHYYFMALKNDEIIDATQKGNCSRFMNHSCEPNCETQKWTVNGQLRVGFFTTKLVPSGSELTFDYQFQRYGKEAQKCFCGSANCRGYLGGENRVSIRAAGGKMKKERSRKKDSVDGELEALMENGEGLSDKNQVLSLSRLMVRIETLEQKLTCLELIQNTHSQSCLKSFLERHGLSLLWIWMAELGDGRESNQKLQEEIIKTLEHLPIPTKNMLEESKVLPIIQRWSQTKTAVPPLSEGDGYSSENTSRAHTPLNTPDPSTKLSTEADTDTPKKLMFRRLKIISENSMDSAISDATSELEGKDGKEDLDQLENVPVEEEEELQSQQLLPQQLPECKVDSETNIEASKLPTSEPEADAEIEPKESNGTKLEEPINEETPSQDEEEGVSDVESERSQEQPDKTVDISDLATKLLDSWKDLKEVYRIPKKSQTEKENTTTERGRDAVGFRDQTPAPKTPNRSRERDPDKQTQNKEKRKRRSSLSPPSSAYERGTKRPDDRYDTPTSKKKVRIKDRNKLSTEERRKLFEQEVAQREAQKQQQQMQNLGMTSPLPYDSLGYNAPHHPFAGYPPGYPMQAYVDPSNPNAGKVLLPTPSMDPVCSPAPYDHAQPLVGHSTEPLSAPPPVPVVPHVAAPVEVSSSQYVAQSDGVVHQDSSVAVLPVPAPGPVQGQNYSVWDSNQQSVSVQQQYSPAQSQATIYYQGQTCPTVYGVTSPYSQTTPPIVQSYAQPSLQYIQGQQIFTAHPQGVVVQPAAAVTTIVAPGQPQPLQPSEMVVTNNLLDLPPPSPPKPKTIVLPPNWKTARDPEGKIYYYHVITRQTQWDPPTWESPGDDASLEHEAEMDLGTPTYDENPMKASKKPKTAEADTSSELAKKSKEVFRKEMSQFIVQCLNPYRKPDCKVGRITTTEDFKHLARKLTHGVMNKELKYCKNPEDLECNENVKHKTKEYIKKYMQKFGAVYKPKEDTELE

翻译修饰 - Q9BYW2 作为底物

Site PTM Type Enzyme
T38 Phosphorylation
K42 Ubiquitination
K47 Methylation
S51 Phosphorylation
S52 Phosphorylation
T126 Phosphorylation
S130 Phosphorylation
S131 Phosphorylation
S301 Phosphorylation
S321 Phosphorylation
S323 Phosphorylation
S327 Phosphorylation
S344 Phosphorylation
K359 Sumoylation
Y377 Phosphorylation
R411 Methylation
S418 Phosphorylation
S422 Phosphorylation
Y427 Phosphorylation
Y428 Phosphorylation
S437 Phosphorylation
S438 Phosphorylation
S458 Phosphorylation
S459 Phosphorylation
S461 Phosphorylation
S470 Phosphorylation
S477 Phosphorylation
Y480 Phosphorylation
T485 Phosphorylation
S532 Phosphorylation
S543 Phosphorylation
Y555 Phosphorylation
K556 Methylation
S572 Phosphorylation
T592 Phosphorylation
S614 Phosphorylation
S618 Phosphorylation
S624 Phosphorylation
T626 Phosphorylation
K629 Ubiquitination
K637 Sumoylation
K637 Ubiquitination
T642 Phosphorylation
S648 Phosphorylation
K650 Ubiquitination
K657 Ubiquitination
K659 Ubiquitination
S676 Phosphorylation
S698 Phosphorylation
S701 Phosphorylation
S708 Phosphorylation
S725 Phosphorylation
S742 Phosphorylation
S744 Phosphorylation
T749 Phosphorylation
S754 Phosphorylation
K776 Ubiquitination
S800 Phosphorylation
S823 Phosphorylation
K899 Ubiquitination
S905 Phosphorylation
S918 Phosphorylation
K922 Ubiquitination
T928 Phosphorylation
S939 Phosphorylation
K941 Acetylation
K941 Ubiquitination
S980 Phosphorylation
S1032 Phosphorylation
T1033 Phosphorylation
S1068 Phosphorylation
T1083 Phosphorylation
S1084 Phosphorylation
S1098 Phosphorylation
Y1113 Phosphorylation
K1116 Ubiquitination
S1119 Phosphorylation
T1137 Phosphorylation
S1144 Phosphorylation
S1149 Phosphorylation
S1165 Phosphorylation
S1178 Phosphorylation
S1186 Phosphorylation
T1189 Phosphorylation
S1196 Phosphorylation
S1206 Phosphorylation
S1207 Phosphorylation
K1215 Ubiquitination
S1216 Phosphorylation
S1228 Phosphorylation
S1236 Phosphorylation
S1263 Phosphorylation
K1266 Ubiquitination
K1285 Ubiquitination
S1366 Phosphorylation
K1399 Acetylation
S1413 Phosphorylation
S1415 Phosphorylation
S1417 Phosphorylation
K1461 Methylation
K1465 Methylation
K1468 Methylation
Y1481 Phosphorylation
T1483 Phosphorylation
K1489 Methylation
K1620 Ubiquitination
R1686 Methylation
S1696 Phosphorylation
S1792 Phosphorylation
K1802 Acetylation
K1812 Acetylation
T1832 Phosphorylation
S1844 Phosphorylation
S1845 Phosphorylation
S1849 Phosphorylation
T1853 Phosphorylation
T1857 Phosphorylation
T1870 Phosphorylation
T1872 Phosphorylation
S1888 Phosphorylation
S1891 Phosphorylation
S1894 Phosphorylation
S1898 Phosphorylation
S1925 Phosphorylation
S1940 Phosphorylation
T1942 Phosphorylation
T1951 Phosphorylation
S1952 Phosphorylation
T1978 Phosphorylation
S1980 Phosphorylation
S1988 Phosphorylation
S1992 Phosphorylation
S1995 Phosphorylation
T2010 Phosphorylation
T2036 Phosphorylation
T2051 Phosphorylation
T2056 Phosphorylation
S2079 Phosphorylation
S2080 Phosphorylation
S2082 Phosphorylation
S2086 Phosphorylation
Y2088 Phosphorylation
T2092 Phosphorylation
Y2099 Phosphorylation
T2101 Phosphorylation
S2104 Phosphorylation
T2421 Phosphorylation
S2424 Phosphorylation
S2430 Phosphorylation
T2441 Phosphorylation
T2443 Phosphorylation
S2464 Phosphorylation
K2546 Acetylation
K2558 Acetylation
T2561 Phosphorylation

研究背景

功能:

Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate. It is capable of trimethylating unmethylated H3K36 (H3K36me0) in vitro. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation (By similarity). Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. Required for DNA double-strand break repair in response to DNA damage: acts by mediating formation of H3K36me3, promoting recruitment of RAD51 and DNA repair via homologous recombination (HR). Acts as a tumor suppressor. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis (By similarity). Required for endoderm development by promoting embryonic stem cell differentiation toward endoderm: acts by mediating formation of H3K36me3 in distal promoter regions of FGFR3, leading to regulate transcription initiation of FGFR3 (By similarity). In addition to histones, also mediates methylation of other proteins, such as tubulins and STAT1. Trimethylates 'Lys-40' of alpha-tubulins such as TUBA1B (alpha-TubK40me3); alpha-TubK40me3 is required for normal mitosis and cytokinesis and may be a specific tag in cytoskeletal remodeling. Involved in interferon-alpha-induced antiviral defense by mediating both monomethylation of STAT1 at 'Lys-525' and catalyzing H3K36me3 on promoters of some interferon-stimulated genes (ISGs) to activate gene transcription.

(Microbial infection) Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.

翻译修饰:

May be automethylated.

细胞定位:

Nucleus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed.

亚基结构:

Specifically interacts with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A): binds to CTD heptad repeats doubly phosphorylated on 'Ser-2' and 'Ser-5' of each heptad. Interacts with HTT. Interacts with IWS1. Interacts with p53/TP53; leading to regulate p53/TP53 target genes. Component of a complex with HNRNPL. Interacts with TUBA1A; the interaction is independent on alpha-tubulin acetylation on 'Lys-40'. Interacts with STAT1.

蛋白家族:

The low charge region mediates the transcriptional activation activity.

The catalytic SET domain binds histone H3 (PubMed:27474439, PubMed:28256625). It is also able to bind oncogenic histone H3 K36M/I found in a number of cancer types, in which histone H3 'Lys-36' is replaced by a Met or an Ile residue. When binding the oncogenic variant histone H3 K36M/I, the SET domain undergoes dramatic conformational change to accommodate the histone H3 peptide, leading to sequester and inhibit SETD2 activity and block global H3K36 methylation (PubMed:27474439, PubMed:28256625).

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.

研究领域

· Metabolism > Amino acid metabolism > Lysine degradation.

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.