Phospho-Annexin A2 (Tyr24) Antibody - #AF7096

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产品: 磷酸化 Annexin A2 (Tyr24) 抗体
货号: AF7096
描述: Rabbit polyclonal antibody to Phospho-Annexin A2 (Tyr24)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 37kDa; 39kD(Calculated).
蛋白号: P07355
RRID: AB_2843536

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(90%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)
克隆:
Polyclonal
特异性:
Phospho-Annexin A2 (Tyr24) Antibody detects endogenous levels of Annexin A2 only when phosphorylated at Tyr24.
RRID:
AB_2843536
引用格式: Affinity Biosciences Cat# AF7096, RRID:AB_2843536.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Annexin A2; Annexin II; Annexin II, heavy chain; Annexin-2; ANX 2; ANX2; ANX2L4; ANXA2; ANXA2_HUMAN; arylsulfatase B; CAL1H; Calpactin I heavy chain; calpactin I heavy polypeptide (p36); Calpactin I heavy polypeptide; Calpactin-1 heavy chain; chromobindin 8; Chromobindin-8; Epididymis secretory protein Li 270; HEL S 270; LIP2; Lipocortin II; LPC2; LPC2D; p36; P36 protein; PAP-IV; Placental anticoagulant protein IV; Protein I;

抗原和靶标

免疫原:
Uniprot:

P07355(ANXA2_HUMAN) >>Visit HPA database.

基因/基因ID:
ANXA2(302)
序列:
MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
90
Horse
90
Chicken
80
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P07355 作为底物

Site PTM Type Enzyme
S2 Phosphorylation
T3 Phosphorylation
K10 Acetylation
K10 Ubiquitination
S12 Phosphorylation P17252 (PRKCA)
S18 Phosphorylation
T19 Phosphorylation
S22 Phosphorylation
Y24 Phosphorylation P12931 (SRC)
S26 Phosphorylation P05771 (PRKCB) , P17252 (PRKCA)
K28 Ubiquitination
Y30 Phosphorylation
T31 Phosphorylation
T44 Phosphorylation
K47 Acetylation
K47 Ubiquitination
K49 Acetylation
K49 Sumoylation
K49 Ubiquitination
T55 Phosphorylation
T61 Phosphorylation
Y75 Phosphorylation
K81 Ubiquitination
S85 Phosphorylation
K88 Ubiquitination
S89 Phosphorylation
S92 Phosphorylation
T97 Phosphorylation
K104 Acetylation
K104 Ubiquitination
T105 Phosphorylation
Y109 Phosphorylation
S112 Phosphorylation
K115 Acetylation
K115 Ubiquitination
S117 Phosphorylation
K119 Ubiquitination
T123 Phosphorylation
S127 Phosphorylation
C133 S-Nitrosylation
S134 Phosphorylation
T136 Phosphorylation
Y147 Phosphorylation
K148 Acetylation
K148 Ubiquitination
K152 Acetylation
K152 Ubiquitination
K157 Acetylation
K157 Ubiquitination
S161 Phosphorylation
T163 Phosphorylation
S164 Phosphorylation
K169 Methylation
K169 Ubiquitination
K176 Acetylation
K176 Ubiquitination
S184 Phosphorylation
Y188 Phosphorylation
Y199 Phosphorylation
K204 Acetylation
K204 Ubiquitination
K212 Acetylation
K212 Ubiquitination
S215 Phosphorylation
T218 Phosphorylation
S221 Phosphorylation
K227 Acetylation
K227 Methylation
K227 Ubiquitination
Y232 Phosphorylation
K233 Acetylation
K233 Ubiquitination
S234 Phosphorylation
Y235 Phosphorylation
S236 Phosphorylation
Y238 Phosphorylation
S243 Phosphorylation
K246 Ubiquitination
K249 Ubiquitination
C262 S-Nitrosylation
K266 Sumoylation
K266 Ubiquitination
Y269 Phosphorylation
Y275 Phosphorylation
S277 Phosphorylation
K279 Acetylation
K279 Ubiquitination
K281 Ubiquitination
S296 Phosphorylation
K302 Acetylation
K302 Ubiquitination
S305 Phosphorylation
K310 Acetylation
K313 Acetylation
K313 Methylation
K313 Ubiquitination
S314 Phosphorylation
Y316 Phosphorylation
Y317 Phosphorylation
Y318 Phosphorylation
T323 Phosphorylation
K324 Acetylation
K324 Sumoylation
K324 Ubiquitination
K329 Ubiquitination
Y333 Phosphorylation

研究背景

功能:

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9.

翻译修饰:

Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.

ISGylated.

细胞定位:

Secreted>Extracellular space>Extracellular matrix>Basement membrane. Melanosome.
Note: In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36 (By similarity). Interacts with ATP1B1 (By similarity). Interacts with DYSF (By similarity). Interacts with COCH. Interacts (via repeat Annexin 1) with PCSK9 (via the C-terminal domain); the interaction inhibits the degradation of LDLR. Interacts with CEACAM1 (via the cytoplasmic domain); this interaction is regulated by phosphorylation of CEACAM1. Interacts with APPL2 and APPL1; targets APPL2 to endosomes and acting in parallel to RAB5A (By similarity).

(Microbial infection) Interacts with human cytomegalovirus (HCMV).

蛋白家族:

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Belongs to the annexin family.

文献引用

1). GNAQ T96S mutation abrogates the ability of wild‐type GNAQ to induce apoptosis by phosphorylating annexin A2 in natural killer/T cell lymphoma. Cancer Science, 2022 (PubMed: 35293080) [IF=5.7]

Application: WB    Species: Mice    Sample: NK cells

FIGURE 4 GNAQ T96S peptide inhibitor induces apoptosis by competing with annexin A2 (ANXA2) binding to GNAQ T96S in natural killer cells. (A) A schematic diagram showing the different domains of GNAQ. (B) Identification of the interaction between different domains of GNAQ and ANXA2 by co‐immunoprecipitation (IP). M or R refers to the host of antibody. (C) GNAQ T96S peptide blocks the binding of GNAQ to ANXA2 in a dose‐dependent manner. (D) GNAQ T96S peptide significantly induces apoptosis in NK cells. (E) GNAQ peptide affects several signaling pathways, including the AKT, ERK, and ANXA2 signaling pathways. The experiment was independently repeated three times
2). (20S) Ginsenoside Rh2 Inhibits STAT3/VEGF Signaling by Targeting Annexin A2. International Journal of Molecular Sciences, 2021 (PubMed: 34502195) [IF=5.6]

Application: WB    Species: Human    Sample: HepG2 cells

igure 1. Anxa2 regulated the expression of VEGFs. HepG2 cells were treated with three different Anxa2-shRNAs. (A) Protein level and phosphorylation status of STAT3 and Anxa2 were determined by immuno-blotting. (B) The activation of STAT3 was determined via reporter gene assay. (C) The expression of STAT3 target genes was determined by qRT-PCR. (D) The protein level of four VEGFs was determined by immuno-blotting. (E) The concentration of four VEGFs in cell-free supernatant was determined by ELISA. All experiments were performed with triple replicant.
3). A novel cystathionine γ-lyase inhibitor, I194496, inhibits the growth and metastasis of human TNBC via downregulating multiple signaling pathways. Scientific Reports, 2021 (PubMed: 33903672) [IF=4.6]

Application: WB    Species: Human    Sample: TNBC cells

Figure 6 I194496 inhibits the Anxa2/STAT3 and VEGF/FAK/Paxillin signalling pathways in human TNBC cells. (A, B) The effect of I194496 on Anxa2/STAT3 pathway in MDA-MB-231 cells. *P 

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