| 产品: | GDF9 抗体 |
| 货号: | AF7535 |
| 描述: | Rabbit polyclonal antibody to GDF9 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 51kDa; 51kD(Calculated). |
| 蛋白号: | O60383 |
| RRID: | AB_2843899 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7535, RRID:AB_2843899.
展开/折叠
GDF9; Growth differentiation factor 9;
抗原和靶标
Expressed in ovarian granulosa cells. Present in oocytes of primary follicles (at protein level).
- O60383 GDF9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARPNKFLLWFCCFAWLCFPISLGSQASGGEAQIAASAELESGAMPWSLLQHIDERDRAGLLPALFKVLSVGRGGSPRLQPDSRALHYMKKLYKTYATKEGIPKSNRSHLYNTVRLFTPCTRHKQAPGDQVTGILPSVELLFNLDRITTVEHLLKSVLLYNINNSVSFSSAVKCVCNLMIKEPKSSSRTLGRAPYSFTFNSQFEFGKKHKWIQIDVTSLLQPLVASNKRSIHMSINFTCMKDQLEHPSAQNGLFNMTLVSPSLILYLNDTSAQAYHSWYSLHYKRRPSQGPDQERSLSAYPVGEEAAEDGRSSHHRHRRGQETVSSELKKPLGPASFNLSEYFRQFLLPQNECELHDFRLSFSQLKWDNWIVAPHRYNPRYCKGDCPRAVGHRYGSPVHTMVQNIIYEKLDSSVPRPSCVPAKYSPLSVLTIEPDGSIAYKEYEDMIATKCTCR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O60383 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S156 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| T189 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| Y394 | Phosphorylation | Uniprot | |
| Y407 | Phosphorylation | Uniprot | |
| Y440 | Phosphorylation | Uniprot |
研究背景
Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.
Phosphorylated; phosphorylation is critical for GDF9 function. In vitro, can be phosphorylated by CK at Ser-325.
Secreted.
Expressed in ovarian granulosa cells. Present in oocytes of primary follicles (at protein level).
Homodimer or heterodimer (Potential). But, in contrast to other members of this family, cannot be disulfide-linked (By similarity).
Belongs to the TGF-beta family.
研究领域
· Organismal Systems > Endocrine system > Ovarian steroidogenesis.
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