| 产品: | SKI 抗体 |
| 货号: | AF7536 |
| 描述: | Rabbit polyclonal antibody to SKI |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Bovine, Horse, Dog, Chicken, Xenopus |
| 分子量: | 80kDa; 80kD(Calculated). |
| 蛋白号: | P12755 |
| RRID: | AB_2843900 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7536, RRID:AB_2843900.
展开/折叠
Avian sarcoma viral (v ski) oncogene homolog; C oncogene; C ski; Proto-oncogene c-Ski; SKI; Ski oncogene; Ski oncoprotein; SKI_HUMAN; SKV; v ski avian sarcoma viral oncogene homolog; v ski sarcoma viral oncogene homolog;
抗原和靶标
- P12755 SKI_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEAAAGGRGCFQPHPGLQKTLEQFHLSSMSSLGGPAAFSARWAQEAYKKESAKEAGAAAVPAPVPAATEPPPVLHLPAIQPPPPVLPGPFFMPSDRSTERCETVLEGETISCFVVGGEKRLCLPQILNSVLRDFSLQQINAVCDELHIYCSRCTADQLEILKVMGILPFSAPSCGLITKTDAERLCNALLYGGAYPPPCKKELAASLALGLELSERSVRVYHECFGKCKGLLVPELYSSPSAACIQCLDCRLMYPPHKFVVHSHKALENRTCHWGFDSANWRAYILLSQDYTGKEEQARLGRCLDDVKEKFDYGNKYKRRVPRVSSEPPASIRPKTDDTSSQSPAPSEKDKPSSWLRTLAGSSNKSLGCVHPRQRLSAFRPWSPAVSASEKELSPHLPALIRDSFYSYKSFETAVAPNVALAPPAQQKVVSSPPCAAAVSRAPEPLATCTQPRKRKLTVDTPGAPETLAPVAAPEEDKDSEAEVEVESREEFTSSLSSLSSPSFTSSSSAKDLGSPGARALPSAVPDAAAPADAPSGLEAELEHLRQALEGGLDTKEAKEKFLHEVVKMRVKQEEKLSAALQAKRSLHQELEFLRVAKKEKLREATEAKRNLRKEIERLRAENEKKMKEANESRLRLKRELEQARQARVCDKGCEAGRLRAKYSAQIEDLQVKLQHAEADREQLRADLLREREAREHLEKVVKELQEQLWPRARPEAAGSEGAAELEP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P12755 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T103 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| K318 | Acetylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | O14965 (AURKA) | Uniprot |
| K335 | Acetylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| S383 | Phosphorylation | O14965 (AURKA) | Uniprot |
| S394 | Phosphorylation | Uniprot | |
| S404 | Phosphorylation | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| T458 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S480 | Phosphorylation | Uniprot | |
| S500 | Phosphorylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| K584 | Ubiquitination | Uniprot | |
| K662 | Ubiquitination | Uniprot | |
| K673 | Ubiquitination | Uniprot | |
| S720 | Phosphorylation | Uniprot |
研究背景
May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.
Ubiquitinated by RNF165, promoting proteasomal degradation, leading to enhance the BMP-Smad signaling.
Nucleus.
Interacts with SMAD2, SMAD3 and SMAD4. Interacts with HIPK2. Part of a complex with HIPK2 and SMAD1/2/3. Interacts with PRDM16 and SMAD3; the interaction with PRDM16 promotes the recruitment SMAD3-HDAC1 complex on the promoter of TGF-beta target genes.
Belongs to the SKI family.
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