HNF1A Antibody - #AF7545

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产品: HNF1A 抗体
货号: AF7545
描述: Rabbit polyclonal antibody to HNF1A
应用: WB
反应: Human, Mouse
预测: Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 56kDa, 70 kDa; 67kD(Calculated).
蛋白号: P20823
RRID: AB_2843909

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
HNF1A Antibody detects endogenous levels of total HNF1A.
RRID:
AB_2843909
引用格式: Affinity Biosciences Cat# AF7545, RRID:AB_2843909.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Albumin proximal factor; Hepatic nuclear factor 1 alpha; Hepatic nuclear factor 1; Hepatic transcription factor 1 alpha; Hepatic transcription factor 1; Hepatocyte nuclear factor 1-alpha; HNF 1; HNF 1A; HNF-1-alpha; HNF-1A; hnf1a; HNF1A_HUMAN; Interferon production regulator factor; LF B1; LF B1 hepatic nuclear factor; LFB 1; LFB1; LFB1 hepatic nuclear factor; Liver specific transcription factor LF B1; Liver specific transcription factor LFB1; Liver-specific transcription factor LF-B1; Maturity onset diabetes of the young 3; MODY 3; MODY3; TCF 1; TCF-1; TCF1; Transcription factor 1; Transcription factor 1 hepatic;

抗原和靶标

免疫原:
Uniprot:

P20823(HNF1A_HUMAN) >>Visit HPA database.

基因/基因ID:
HNF1A(6927)
表达:
P20823 HNF1A_HUMAN:

Liver.

序列:
MVSKLSQLQTELLAALLESGLSKEALIQALGEPGPYLLAGEGPLDKGESCGGGRGELAELPNGLGETRGSEDETDDDGEDFTPPILKELENLSPEEAAHQKAVVETLLQEDPWRVAKMVKSYLQQHNIPQREVVDTTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREVAQQFTHAGQGGLIEEPTGDELPTKKGRRNRFKWGPASQQILFQAYERQKNPSKEERETLVEECNRAECIQRGVSPSQAQGLGSNLVTEVRVYNWFANRRKEEAFRHKLAMDTYSGPPPGPGPGPALPAHSSPGLPPPALSPSKVHGVRYGQPATSETAEVPSSSGGPLVTVSTPLHQVSPTGLEPSHSLLSTEAKLVSAAGGPLPPVSTLTALHSLEQTSPGLNQQPQNLIMASLPGVMTIGPGEPASLGPTFTNTGASTLVIGLASTQAQSVPVINSMGSSLTTLQPVQFSQPLHPSYQQPLMPPVQSHVTQSPFMATMAQLQSPHALYSHKPEVAQYTHTGLLPQTMLITDTTNLSALASLTPTKQVFTSDTEASSESGLHTPASQATTLHVPSQDPAGIQHLQPAHRLSASPTVSSSSLVLYQSSDSSNGQSHLLPSNHSVIETFISTQMASSSQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Pig
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P20823 作为底物

Site PTM Type Enzyme
T67 Phosphorylation
S70 Phosphorylation
T74 Phosphorylation
T82 Phosphorylation
S93 Phosphorylation
T196 Phosphorylation
S247 Phosphorylation
S249 Phosphorylation Q9Y463 (DYRK1B) , Q13315 (ATM)

研究背景

功能:

Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity).

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Liver.

亚基结构:

Binds DNA as a dimer. Heterotetramer with PCBD1; formed by a dimer of dimers (By similarity). Interacts with PCBD1. Interacts with BHLHE41 (By similarity).

蛋白家族:

Belongs to the HNF1 homeobox family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

文献引用

1). Acetaminophen-induced hepatocyte injury: C2-ceramide and oltipraz intervention, hepatocyte nuclear factor 1 and glutathione S-transferase A1 changes. JOURNAL OF APPLIED TOXICOLOGY, 2019 (PubMed: 31385618) [IF=3.3]

Application: WB    Species:    Sample: hepatocytes

FIGURE 6| A, Original blots for HNF‐1, GSTA1 and β‐actin. B, HNF‐1 protein level. C, GSTA1 protein level. Changes in HNF‐1 and GSTA1 protein levels in hepatocytes after 15 mmol/L APAP exposure for 10 h with C2 (6 μmol/L) or OL (8 μmol/L). Values are expressed as the mean ± SD for each group (n = 4). *P < .05, **P < .01 represented all groups compared with the control group; #P < .05, ##P < .01 represented all groups compared with the APAP model group. APAP, acetaminophen; C2, C2‐ceramide; GSTA1, glutathione S‐transferase A1; HNF‐1, hepatocyte nuclear factor 1; OL, oltipraz
2). Acetaminophen-induced reduction in glutathione-S-transferase A1 in hepatocytes: A role for hepatic nuclear factor 1α and its response element. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2019 (PubMed: 31230750) [IF=3.1]

Application: WB    Species: human    Sample: HepG2 cells

Fig. 3. |Effects of C2-ceramide or oltipraz on the nuclear HNF-1a levels and the GSTA1 protein expression in HepG2 cells with injury induced by APAP (n ¼ 4). *P < 0.05, **P < 0.01 compared with control group; #P < 0.05, ##P < 0.01 compared with model group.

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