SLC32A1 Antibody | Affinity Biosciences LTD|亲科生物官网

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Western blot analysis of extracts from Hela cells, using SLC32A1 Antibody.

Fig.

产品:	SLC32A1 抗体
货号:	AF7551
描述:	Rabbit polyclonal antibody to SLC32A1
应用:	WB
反应:	Human, Mouse, Rat
预测:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量:	57kDa; 57kD(Calculated).
蛋白号:	Q9H598
RRID:	AB_2843915

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

克隆:

Polyclonal

特异性:

SLC32A1 Antibody detects endogenous levels of total SLC32A1.

RRID:

AB_2843915
引用格式: Affinity Biosciences Cat# AF7551, RRID:AB_2843915.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

bA122O1.1; GABA and glycine transporter; hVIAAT; SLC32A 1; Slc32a1; solute carrier family 32 (GABA vesicular transporter) member 1; Solute carrier family 32 member 1; Vesicular GABA Amino Acid Transporter; Vesicular GABA transporter; Vesicular inhibitory amino acid transporter; VGAT; VIAAT; VIAAT_HUMAN;

抗原和靶标

免疫原:

Uniprot:

Q9H598(VIAAT_HUMAN) >>Visit HPA database.

基因/基因ID:

SLC32A1(140679)

表达:

Q9H598 VIAAT_HUMAN:

Retina. Expressed throughout the horizontal cells or more specifically at the terminals.

序列:

Q9H598 VIAAT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATLLRSKLSNVATSVSNKSQAKMSGMFARMGFQAATDEEAVGFAHCDDLDFEHRQGLQMDILKAEGEPCGDEGAEAPVEGDIHYQRGSGAPLPPSGSKDQVGGGGEFGGHDKPKITAWEAGWNVTNAIQGMFVLGLPYAILHGGYLGLFLIIFAAVVCCYTGKILIACLYEENEDGEVVRVRDSYVAIANACCAPRFPTLGGRVVNVAQIIELVMTCILYVVVSGNLMYNSFPGLPVSQKSWSIIATAVLLPCAFLKNLKAVSKFSLLCTLAHFVINILVIAYCLSRARDWAWEKVKFYIDVKKFPISIGIIVFSYTSQIFLPSLEGNMQQPSEFHCMMNWTHIAACVLKGLFALVAYLTWADETKEVITDNLPGSIRAVVNIFLVAKALLSYPLPFFAAVEVLEKSLFQEGSRAFFPACYSGDGRLKSWGLTLRCALVVFTLLMAIYVPHFALLMGLTGSLTGAGLCFLLPSLFHLRLLWRKLLWHQVFFDVAIFVIGGICSVSGFVHSLEGLIEAYRTNAED

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Rabbit

100

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9H598 作为底物

Q9H598

Site	PTM Type	Source
S17	Phosphorylation	Uniprot
S20	Phosphorylation	Uniprot
S96	Phosphorylation	Uniprot
S98	Phosphorylation	Uniprot
Y300	Phosphorylation	Uniprot
T443	Phosphorylation	Uniprot
S462	Phosphorylation	Uniprot

研究背景

Q9H598_VIAAT_HUMAN

功能:

Involved in the uptake of GABA and glycine into the synaptic vesicles.

细胞定位:

Cytoplasmic vesicle membrane>Multi-pass membrane protein.

组织特异性:

Retina. Expressed throughout the horizontal cells or more specifically at the terminals.

蛋白家族:

Belongs to the amino acid/polyamine transporter 2 family.

研究领域

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)

· Organismal Systems > Nervous system > GABAergic synapse.

文献引用

1). Increased Fibronectin Impairs the Function of Excitatory/Inhibitory Synapses in Hirschsprung Disease. CELLULAR AND MOLECULAR NEUROBIOLOGY, 2020 (PubMed: 31760535) [IF=4.0]

Application: WB Species: rat Sample: PC12 cell

Application: IF/ICC Species: rat Sample: PC12 cell

Fig. 3|Suppression of FN increases the expression of synaptic proteins. A Relative expression of NL-1, PSD-95, vGLUT1, NL-2, SLC32, and FN mRNAs were signifcantly increased in the FN-KD group compared with the NC group and B Western blots of PC12 cell lysates in the FN-KD, NC, and Ctr groups with NL-1, PSD-95, NL-2, SLC32, and FN antibodies. The quantitative results (right) confrm that silencing FN signifcantly increases the expression of neuronal marker proteins.C The co-expression of NL-1, or NL-2, or PSD-95, or SLC32 with FN was examined by double immunofuorescence staining. The results support the idea that downregulation of FN enhances NL-1, PSD-95, NL-2, and SLC32 expression. Values are mean ± SEM, *P < 0.05, **P < 0.01, and ***P < 0.001

Application: IHC Species: human Sample: ganglionic, transitional, and aganglionic colonic segments

Fig. 2|Quantitative histological evaluations of ganglionic, transitional, and aganglionic colonic segments of HSCR patients. A1–3, a1–3 PSD-95 group, B1–3, b1–3 SLC32 group, C1–3, c1–3 FN group, d histological score, based on the intensity of positive staining (brown), and e area of positive staining shown as a percentage of total tissue area. Figures are representative of at least fve separate experiments. The diferences among the three groups were all statistically signifcant. Values are mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (ganglionic vs. aganglionic, P < 0.0001)

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SLC32A1 Antibody - #AF7551

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