| 产品: | EED 抗体 |
| 货号: | AF7558 |
| 描述: | Rabbit polyclonal antibody to EED |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 50kDa; 50kD(Calculated). |
| 蛋白号: | O75530 |
| RRID: | AB_2843922 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7558, RRID:AB_2843922.
展开/折叠
eed; EED protein; EED, mouse, homolog of; EED_HUMAN; Embryonic ectoderm development; Embryonic ectoderm development isoform a; Embryonic ECTODERM development protein homolog; Embryonic ectoderm development protein, mouse, homolog of; hEED; OTTHUMP00000235483; OTTHUMP00000235484; OTTHUMP00000235485; Polycomb protein eed; WAIT 1; WAIT-1; WAIT1; WD protein associating with integrin cytoplasmic tails 1;
抗原和靶标
Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
- O75530 EED_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75530 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| K19 | Acetylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| T50 | Phosphorylation | Uniprot | |
| T55 | Phosphorylation | Uniprot | |
| T57 | Phosphorylation | Uniprot | |
| T59 | Phosphorylation | Uniprot | |
| K66 | Methylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| T115 | Phosphorylation | Uniprot | |
| K197 | Methylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K268 | Methylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| Y278 | Phosphorylation | Uniprot | |
| Y280 | Phosphorylation | Uniprot | |
| K284 | Methylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| K408 | Ubiquitination | Uniprot |
研究背景
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines.
Nucleus. Chromosome.
Note: Transiently colocalizes with XIST at inactive X chromosomes.
Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC, HDAC2, histone H1 and YY1. May interact with ITGA4, ITGAE and ITGB7. Interacts with CDYL. Interacts with ARNTL/BMAL1. Interacts with KMT2A/MLL1 (By similarity).
(Microbial infection) May interact with the MA protein of HIV-1.
The WD repeat domain mediates recognition of trimethylated histone peptides at the consensus sequence Ala-Arg-Lys-Ser. This is achieved through an aromatic cage encircling the methyllysine, and involving Phe-97, Tyr-148 and Tyr-365.
Belongs to the WD repeat ESC family.
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