CDC5L Antibody - #AF7563

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产品: CDC5L 抗体
货号: AF7563
描述: Rabbit polyclonal antibody to CDC5L
应用: WB
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 92kDa; 92kD(Calculated).
蛋白号: Q99459
RRID: AB_2843927

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Bovine(90%), Horse(100%), Sheep(90%), Rabbit(90%), Dog(100%), Chicken(90%), Xenopus(80%)
克隆:
Polyclonal
特异性:
CDC5L Antibody detects endogenous levels of total CDC5L.
RRID:
AB_2843927
引用格式: Affinity Biosciences Cat# AF7563, RRID:AB_2843927.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CDC5; CDC5 cell division cycle 5-like (S. pombe); Cdc5 related protein; CDC5-LIKE; Cdc5-like protein; Cdc5l; CDC5L_HUMAN; CEF1; Cell division cycle 5 S. pombe homolog of; Cell division cycle 5-like protein; dJ319D22.1; KIAA0432; PCDC5RP; Pombe Cdc5 related protein; Pombe Cdc5-related protein;

抗原和靶标

免疫原:
Uniprot:

Q99459(CDC5L_HUMAN) >>Visit HPA database.

基因/基因ID:
CDC5L(988)
表达:
Q99459 CDC5L_HUMAN:

Ubiquitously expressed in both fetal and adult tissues.

序列:
MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKKTEWSREEEEKLLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDPRKLKPGEIDPNPETKPARPDPIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEARRLAALQKRRELRAAGIEIQKKRKRKRGVDYNAEIPFEKKPALGFYDTSEENYQALDADFRKLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAILQTSGVSEFTKKRSKLVLPAPQISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVALRTPRTPASQDRILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRTPSNGAEGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKSKF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Dog
100
Bovine
90
Sheep
90
Chicken
90
Rabbit
90
Xenopus
80
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q99459 作为底物

Site PTM Type Enzyme
K25 Ubiquitination
K59 Ubiquitination
K70 Ubiquitination
K165 Methylation
K166 Acetylation
K168 Acetylation
K187 Ubiquitination
Y210 Phosphorylation
K219 Sumoylation
K219 Ubiquitination
Y225 Phosphorylation
T227 Phosphorylation
S228 Phosphorylation
Y232 Phosphorylation
R252 Methylation
S253 Phosphorylation
S273 Phosphorylation
S277 Phosphorylation
S303 Phosphorylation
S317 Phosphorylation
T344 Phosphorylation
S347 Phosphorylation
T352 Phosphorylation
T355 Phosphorylation
S358 Phosphorylation
T373 Phosphorylation
T377 Phosphorylation
K380 Ubiquitination
T385 Phosphorylation
S390 Phosphorylation
S393 Phosphorylation
T396 Phosphorylation
T404 Phosphorylation
T407 Phosphorylation
S410 Phosphorylation P24941 (CDK2)
T411 Phosphorylation P24941 (CDK2)
T415 Phosphorylation
S417 Phosphorylation
T424 Phosphorylation
S427 Phosphorylation
T429 Phosphorylation
T430 Phosphorylation
K432 Ubiquitination
S437 Phosphorylation
T438 Phosphorylation
T442 Phosphorylation
K447 Ubiquitination
Y459 Phosphorylation
S460 Phosphorylation
S463 Phosphorylation
Y464 Phosphorylation
K466 Ubiquitination
K487 Ubiquitination
K500 Ubiquitination
T510 Phosphorylation
Y511 Phosphorylation
Y621 Phosphorylation
K623 Ubiquitination
K626 Acetylation
S691 Phosphorylation
K694 Ubiquitination
Y788 Phosphorylation
K795 Ubiquitination
T797 Phosphorylation

研究背景

功能:

DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).

翻译修饰:

Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.

细胞定位:

Nucleus. Nucleus speckle. Cytoplasm.
Note: May shuttle between cytoplasm and nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed in both fetal and adult tissues.

亚基结构:

Homodimer. Interacts with DAPK3 (By similarity). Component of the precatalytic, catalytic and postcatalytic spliceosome complexes. Part of a spliceosomal 'core' complex consisting of CDC5L, PLRG1, SPF27, CCAP1, CCAP3 and CCAP6. Interacts with PLRG1, Lodestar/TTF2, and NIPP1/PPP1R8. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its C-terminus) directly in the complex with PRPF19 and BCAS2. Interacts (via its C-terminus) directly with PRGL1 (via its WD40 repeat domain); the interaction is required for mRNA splicing but not for spliceosome assembly. Also interacts with CTNNBL1. Interacts with PRPF19 (via N-terminus) (By similarity).

蛋白家族:

Belongs to the CEF1 family.

研究领域

· Genetic Information Processing > Transcription > Spliceosome.

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