| 产品: | ZEB1 抗体 |
| 货号: | AF7564 |
| 描述: | Rabbit polyclonal antibody to ZEB1 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 124kDa; 124kD(Calculated). |
| 蛋白号: | P37275 |
| RRID: | AB_2843928 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7564, RRID:AB_2843928.
展开/折叠
AREB 6; BZP; Delta crystallin enhancer binding factor 1; DELTA EF1; FECD6; MGC133261; Negative regulator of IL 2; Negative regulator of IL2; NIL 2 A; NIL 2 A zinc finger protein; NIL 2A; NIL-2-A zinc finger protein; NIL2A; Posterior polymorphous corneal dystrophy 3; PPCD3; Represses interleukin 2 expression; TCF 8; TCF-8; TCF8; Transcription factor 8 (represses interleukin 2 expression); Transcription factor 8; ZEB 1; ZEB; ZEB1; ZEB1_HUMAN; ZFHEP; ZFHX 1A; ZFHX1A; Zinc finger E box binding homeobox 1; Zinc finger E-box-binding homeobox 1; Zinc finger homeodomain enhancer binding protein;
抗原和靶标
Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
- P37275 ZEB1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADGPRCKRRKQANPRRNNVTNYNTVVETNSDSDDEDKLHIVEEESVTDAADCEGVPEDDLPTDQTVLPGRSSEREGNAKNCWEDDRKEGQEILGPEAQADEAGCTVKDDECESDAENEQNHDPNVEEFLQQQDTAVIFPEAPEEDQRQGTPEASGHDENGTPDAFSQLLTCPYCDRGYKRFTSLKEHIKYRHEKNEDNFSCSLCSYTFAYRTQLERHMTSHKSGRDQRHVTQSGCNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCISLIPVNGRPRTGLKTSQCSSPSLSASPGSPTRPQIRQKIENKPLQEQLSVNQIKTEPVDYEFKPIVVASGINCSTPLQNGVFTGGGPLQATSSPQGMVQAVVLPTVGLVSPISINLSDIQNVLKVAVDGNVIRQVLENNQANLASKEQETINASPIQQGGHSVISAISLPLVDQDGTTKIIINYSLEQPSQLQVVPQNLKKENPVATNSCKSEKLPEDLTVKSEKDKSFEGGVNDSTCLLCDDCPGDINALPELKHYDLKQPTQPPPLPAAEAEKPESSVSSATGDGNLSPSQPPLKNLLSLLKAYYALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQAGQISVQSSEPSSPEPGKVNIPAKNNDQPQSANANEPQDSTVNLQSPLKMTNSPVLPVGSTTNGSRSSTPSPSPLNLSSSRNTQGYLYTAEGAQEEPQVEPLDLSLPKQQGELLERSTITSVYQNSVYSVQEEPLNLSCAKKEPQKDSCVTDSEPVVNVIPPSANPINIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQTILIPQVAYTYSTTVSPAVQEPPLKVIQPNGNQDERQDTSSEGVSNVEDQNDSDSTPPKKKMRKTENGMYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICKKAFKHKHHLIEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEERDSTEQEEAGPEILSNEHVGARASPSQGDSDERESLTREEDEDSEKEEEEEDKEMEELQEEKECEKPQGDEEEEEEEEEVEEEEVEEAENEGEEAKTEGLMKDDRAESQASSLGQKVGESSEQVSEEKTNEA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P37275 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S31 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| T151 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| T162 | Phosphorylation | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| T243 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| T324 | Phosphorylation | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| K347 | Sumoylation | Uniprot | |
| T443 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| K493 | Sumoylation | Uniprot | |
| K504 | Sumoylation | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| K507 | Ubiquitination | Uniprot | |
| K515 | Sumoylation | Uniprot | |
| K515 | Ubiquitination | Uniprot | |
| S516 | Phosphorylation | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| S529 | Phosphorylation | Uniprot | |
| S583 | Phosphorylation | Uniprot | |
| S585 | Phosphorylation | Uniprot | |
| K613 | Ubiquitination | Uniprot | |
| S641 | Phosphorylation | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| S645 | Phosphorylation | Uniprot | |
| S646 | Phosphorylation | Uniprot | |
| S679 | Phosphorylation | Uniprot | |
| S686 | Phosphorylation | Uniprot | |
| S693 | Phosphorylation | Uniprot | |
| S698 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| S701 | Phosphorylation | Uniprot | |
| T702 | Phosphorylation | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| S706 | Phosphorylation | Uniprot | |
| T716 | Phosphorylation | Uniprot | |
| S850 | Phosphorylation | Uniprot | |
| S887 | Phosphorylation | Uniprot | |
| S889 | Phosphorylation | Uniprot | |
| T890 | Phosphorylation | Uniprot | |
| T927 | Phosphorylation | Uniprot | |
| S955 | Phosphorylation | Uniprot | |
| S995 | Phosphorylation | Uniprot | |
| T996 | Phosphorylation | Uniprot | |
| S1016 | Phosphorylation | Uniprot | |
| S1018 | Phosphorylation | Uniprot | |
| S1022 | Phosphorylation | Uniprot | |
| S1036 | Phosphorylation | Uniprot | |
| S1100 | Phosphorylation | Uniprot | |
| K1108 | Ubiquitination | Uniprot |
研究背景
Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.
Nucleus.
Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
Interacts (via N-terminus) with SMARCA4/BRG1.
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
研究领域
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)
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