| 产品: | TRIM32 抗体 |
| 货号: | AF7574 |
| 描述: | Rabbit polyclonal antibody to TRIM32 |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 72kDa; 72kD(Calculated). |
| 蛋白号: | Q13049 |
| RRID: | AB_2843938 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7574, RRID:AB_2843938.
展开/折叠
72 kda Tat interacting Protein; 72 kDa Tat-interacting protein; BBS11; E3 ubiquitin-protein ligase TRIM32; HT2A; LGMD2H; Limb girdle muscular dystrophy 2H (autosomal recessive); Limb girdle muscular dystrophy 2H; Muscular dystrophy Hutterite type; TAT interactive protein 72KD; TATIP; TRI32_HUMAN; Trim32; Tripartite Motif Containing Protein 32; Tripartite motif-containing protein 32; Zinc finger protein HT2A;
抗原和靶标
- Q13049 TRI32_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAAASHLNLDALREVLECPICMESFTEEQLRPKLLHCGHTICRQCLEKLLASSINGVRCPFCSKITRITSLTQLTDNLTVLKIIDTAGLSEAVGLLMCRSCGRRLPRQFCRSCGLVLCEPCREADHQPPGHCTLPVKEAAEERRRDFGEKLTRLRELMGELQRRKAALEGVSKDLQARYKAVLQEYGHEERRVQDELARSRKFFTGSLAEVEKSNSQVVEEQSYLLNIAEVQAVSRCDYFLAKIKQADVALLEETADEEEPELTASLPRELTLQDVELLKVGHVGPLQIGQAVKKPRTVNVEDSWAMEATASAASTSVTFREMDMSPEEVVASPRASPAKQRGPEAASNIQQCLFLKKMGAKGSTPGMFNLPVSLYVTSQGEVLVADRGNYRIQVFTRKGFLKEIRRSPSGIDSFVLSFLGADLPNLTPLSVAMNCQGLIGVTDSYDNSLKVYTLDGHCVACHRSQLSKPWGITALPSGQFVVTDVEGGKLWCFTVDRGSGVVKYSCLCSAVRPKFVTCDAEGTVYFTQGLGLNLENRQNEHHLEGGFSIGSVGPDGQLGRQISHFFSENEDFRCIAGMCVDARGDLIVADSSRKEILHFPKGGGYSVLIREGLTCPVGIALTPKGQLLVLDCWDHCIKIYSYHLRRYSTP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13049 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S7 | Phosphorylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| Y241 | Phosphorylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K247 | Ubiquitination | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| T321 | Phosphorylation | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| K471 | Ubiquitination | Uniprot | |
| K506 | Ubiquitination | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| K604 | Ubiquitination | Uniprot | |
| T617 | Phosphorylation | Uniprot | |
| K627 | Ubiquitination | Uniprot | |
| S651 | Phosphorylation | Uniprot |
研究背景
Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.
Ubiquitinated.
Cytoplasm.
Note: Localized in cytoplasmic bodies, often located around the nucleus.
Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.
Interacts with DTNBP1. It self-associates.
Belongs to the TRIM/RBCC family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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