| 产品: | CUL4B 抗体 |
| 货号: | AF7583 |
| 描述: | Rabbit polyclonal antibody to CUL4B |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Sheep, Rabbit, Dog |
| 分子量: | 99kDa; 104kD(Calculated). |
| 蛋白号: | Q13620 |
| RRID: | AB_2843947 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7583, RRID:AB_2843947.
展开/折叠
CUL 4B; CUL-4B; CUL4B; CUL4B_HUMAN; Cullin-4B; DKFZp686F1470; KIAA0695; MRXHF2; MRXSC; SFM2;
抗原和靶标
- Q13620 CUL4B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMSQSSGSGDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSSNEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSSSSSSSSPTAATSQQQQLKNKSILISSVASVHHANGLAKSSTTVSSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFREDSLDSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQNKTIDGILLLIERERNGEAIDRSLLRSLLSMLSDLQIYQDSFEQRFLEETNRLYAAEGQKLMQEREVPEYLHHVNKRLEEEADRLITYLDQTTQKSLIATVEKQLLGEHLTAILQKGLNNLLDENRIQDLSLLYQLFSRVRGGVQVLLQQWIEYIKAFGSTIVINPEKDKTMVQELLDFKDKVDHIIDICFLKNEKFINAMKEAFETFINKRPNKPAELIAKYVDSKLRAGNKEATDEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMIQFKQYMQNQNVPGNIELTVNILTMGYWPTYVPMEVHLPPEMVKLQEIFKTFYLGKHSGRKLQWQSTLGHCVLKAEFKEGKKELQVSLFQTLVLLMFNEGEEFSLEEIKQATGIEDGELRRTLQSLACGKARVLAKNPKGKDIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQASTTERVFQDRQYQIDAAIVRIMKMRKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYNYIA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13620 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T46 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| T48 | Phosphorylation | Uniprot | |
| T49 | Phosphorylation | Uniprot | |
| T52 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| T85 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| T148 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| K158 | Methylation | Uniprot | |
| K160 | Methylation | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| K190 | Acetylation | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K258 | Methylation | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| Y413 | Phosphorylation | Uniprot | |
| K419 | Ubiquitination | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| K539 | Ubiquitination | Uniprot | |
| K558 | Ubiquitination | Uniprot | |
| K565 | Ubiquitination | Uniprot | |
| K570 | Ubiquitination | Uniprot | |
| K585 | Ubiquitination | Uniprot | |
| Y598 | Phosphorylation | Uniprot | |
| Y607 | Phosphorylation | Uniprot | |
| K608 | Ubiquitination | Uniprot | |
| K619 | Ubiquitination | Uniprot | |
| K634 | Ubiquitination | Uniprot | |
| K644 | Ubiquitination | Uniprot | |
| K650 | Ubiquitination | Uniprot | |
| R779 | Methylation | Uniprot | |
| C787 | S-Nitrosylation | Uniprot | |
| K789 | Ubiquitination | Uniprot | |
| K800 | Acetylation | Uniprot | |
| K807 | Ubiquitination | Uniprot | |
| K815 | Methylation | Uniprot | |
| K815 | Ubiquitination | Uniprot | |
| K829 | Methylation | Uniprot | |
| R846 | Methylation | Uniprot | |
| Y874 | Phosphorylation | Uniprot | |
| K882 | Ubiquitination | Uniprot | |
| S892 | Phosphorylation | Uniprot | |
| R896 | Methylation | Uniprot | |
| Y898 | Phosphorylation | Uniprot | |
| K903 | Ubiquitination | Uniprot |
研究背景
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8. With CUL4A, contributes to ribosome biogenesis.
Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
Nucleus.
Component of multiple DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes that seem to be formed of DDB1, CUL4A or CUL4B, RBX1 and a variable substrate recognition component which seems to belong to a protein family described as DCAF (Ddb1- and Cul4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins. Component of the DCX(DTL) complex with the putative substrate recognition component DTL. Component of the DCX(DDB2) complex with the putative substrate recognition component DDB2. Part of a complex with RBX1 and TIP120A/CAND1. Interacts with RBX1 GRWD1, MLST8, SMU1, TLE2, TLE3, DCAF1, DDA1, DCAF6, DCAF17, DDB2, DCAF8, TIP120A/CAND1 and TMEM113. Interacts with cyclin E and with importins alpha-1 (KPNA2), alpha-3 (KPNA4), alpha-5 (KPNA1) and beta-1 (KPNB1). May interact with WDR26, WDR51B, SNRNP40, WDR61, WDR76 and WDR5.
Belongs to the cullin family.
研究领域
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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