PAX7 Antibody - #AF7584

Figure 2 Generation of Tmem30a conditional knockout (cKO) mice A: Breeding strategy of cKO mice by crossing Tmem30aloxP/loxP with Pax7CreER mice. B: Genotyping of mice by PCR and agarose gel electrophoresis. C: Axis of tamoxifen intraperitoneal injection, BaCl2 injection, and TA muscle harvesting in mice. D: In 7 dpi cKO mice, expression of Pax7CreER was monitored by ROSA-tdTomato reporter (red). SCs were labeled with Pax7 antibody (green). Nuclei were counterstained with DAPI (blue). Scale bar: 50 μm. E: TMEM30A protein from 7 dpi WT and cKO mice was labeled with TMEM30A antibody (green) and DAPI (blue). Scale bar: 50 μm. F, G: Immunoblot analysis of TMEM30A protein expression in 7 dpi WT and cKO mice. Relative protein band density was calculated by ImageJ. Data are mean±SEM. n=3 in each group. Significance was calculated by two-tailed Student’s t-test. *: P<0.05.

Fig. 2.Bi-phasic effect of gelatin in activating quiescent satellite cells (SCs) and myogenesis during muscle regeneration. (A) Immunohistochemical (IHC) analysis of Pax7+ SCs (green) in TA muscle stained by laminin (red) at 7 D.P.I. Arrows point to Pax7+ nuclei. Scale bar: 50 μm. (B) The percentage of myofibers containing Pax7+ SCs (n=6). (C) IHC analysis of MyoG+ cells (green) in damaged TA muscle stained by laminin (red) at 7 D.P.I. Arrows point to MyoG+ nuclei. Scale bar: 50 μm. (D) The percentage of myofibers containing MyoG+ cells (n=6). (E,F) Western blots (E) and quantification (F) of MyoD and MyoG in damaged TA muscles at 14 D.P.I. (n=3). GAPDH is used as a loading control. Significance was determined by unpaired two-tailed Student's t-test with Welch's correction.