| 产品: | LPIN1 抗体 |
| 货号: | AF7585 |
| 描述: | Rabbit polyclonal antibody to LPIN1 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 99kDa; 99kD(Calculated). |
| 蛋白号: | Q14693 |
| RRID: | AB_2843949 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7585, RRID:AB_2843949.
展开/折叠
EC=3.1.3.4; KIAA0188; Lipin-1; Lpin1; LPIN1_HUMAN; PAP1; Phosphatidate phosphatase LPIN1;
抗原和靶标
Specifically expressed in skeletal muscle. Also abundant in adipose tissue. Lower levels in some portions of the digestive tract.
- Q14693 LPIN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNYVGQLAGQVFVTVKELYKGLNPATLSGCIDIIVIRQPNGNLQCSPFHVRFGKMGVLRSREKVVDIEINGESVDLHMKLGDNGEAFFVQETDNDQEVIPMHLATSPILSEGASRMECQLKRGSVDRMRGLDPSTPAQVIAPSETPSSSSVVKKRRKRRRKSQLDSLKRDDNMNTSEDEDMFPIEMSSDEAMELLESSRTLPNDIPPFQDDIPEENLSLAVIYPQSASYPNSDREWSPTPSPSGSRPSTPKSDSELVSKSTERTGQKNPEMLWLWGELPQAAKSSSPHKMKESSPLSSRKICDKSHFQAIHSESSDTFSDQSPTLVGGALLDQNKPQTEMQFVNEEDLETLGAAAPLLPMIEELKPPSASVVQTANKTDSPSRKRDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGDPSGLAKHASDNGARSANQSPQSVGSSGVDSGVESTSDGLRDLPSIAISLCGGLSDHREITKDAFLEQAVSYQQFVDNPAIIDDPNLVVKIGSKYYNWTTAAPLLLAMQAFQKPLPKATVESIMRDKMPKKGGRWWFSWRGRNTTIKEESKPEQCLAGKAHSTGEQPPQLSLATRVKHESSSSDEERAAAKPSNAGHLPLLPNVSYKKTLRLTSEQLKSLKLKNGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKVIISDIDGTITRSDTLGHILPTLGKDWTHQGIAKLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTVLPQGPLLLSPSSLFSALHREVIEKKPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVYSYKQVGVSLNRIFTVNPKGELVQEHAKTNISSYVRLCEVVDHVFPLLKRSHSSDFPCSDTFSNFTFWREPLPPFENQDIHSASA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q14693 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T14 | Phosphorylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| S106 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S134 | Phosphorylation | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| T145 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| T175 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| T249 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S449 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| T563 | Phosphorylation | Uniprot | |
| S580 | Phosphorylation | Uniprot | |
| K609 | Ubiquitination | Uniprot | |
| K636 | Ubiquitination | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| K707 | Ubiquitination | Uniprot | |
| K711 | Ubiquitination | Uniprot | |
| S713 | Phosphorylation | Uniprot | |
| Y717 | Phosphorylation | Uniprot | |
| K770 | Ubiquitination | Uniprot | |
| K776 | Ubiquitination | Uniprot | |
| K854 | Ubiquitination | Uniprot | |
| S887 | Phosphorylation | Uniprot | |
| S889 | Phosphorylation | Uniprot |
研究背景
Plays important roles in controlling the metabolism of fatty acids at different levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol (By similarity).
Phosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway. Phosphorylation is decreased by epinephrine. Phosphorylation may not directly affect the catalytic activity but may regulate the localization. Dephosphorylated by the CTDNEP1-CNEP1R1 complex (By similarity).
Sumoylated.
Nucleus membrane. Cytoplasm>Cytosol. Endoplasmic reticulum membrane.
Specifically expressed in skeletal muscle. Also abundant in adipose tissue. Lower levels in some portions of the digestive tract.
Interacts (via LXXIL motif) with PPARA. Interacts with PPARGC1A. Interaction with PPARA and PPARGC1A leads to the formation of a complex that modulates gene transcription. Interacts with MEF2C.
Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL), a transcriptional binding motif, which mediates interaction with PPARA.
Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity.
Belongs to the lipin family.
研究领域
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Metabolism > Lipid metabolism > Glycerolipid metabolism.
· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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