ABCB11 Antibody - #AF7593

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产品: ABCB11 抗体
货号: AF7593
描述: Rabbit polyclonal antibody to ABCB11
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 146kDa; 146kD(Calculated).
蛋白号: O95342
RRID: AB_2843957

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MS Report
HPLC Report
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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(90%), Bovine(90%), Horse(80%), Sheep(90%), Rabbit(80%), Dog(80%)
克隆:
Polyclonal
特异性:
ABCB11 Antibody detects endogenous levels of total ABCB11.
RRID:
AB_2843957
引用格式: Affinity Biosciences Cat# AF7593, RRID:AB_2843957.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ABC member 16, MDR/TAP subfamily; ABC16; ABCB1; Abcb11; ABCBB_HUMAN; ATP binding cassette sub family B (MDR/TAP) member 11; ATP binding cassette sub family B member 11; ATP-binding cassette sub-family B member 11; Bile salt export pump; BRIC2; Bsep; PFIC 2; PFIC2; PGY4; progressive familial intrahepatic cholestasis 2; Sister of P glycoprotein; Spgp;

抗原和靶标

免疫原:
Uniprot:

O95342(ABCBB_HUMAN) >>Visit HPA database.

基因/基因ID:
ABCB11(8647)
表达:
O95342 ABCBB_HUMAN:

Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.

序列:
MSDSVILRSIKKFGEENDGFESDKSYNNDKKSRLQDEKKGDGVRVGFFQLFRFSSSTDIWLMFVGSLCAFLHGIAQPGVLLIFGTMTDVFIDYDVELQELQIPGKACVNNTIVWTNSSLNQNMTNGTRCGLLNIESEMIKFASYYAGIAVAVLITGYIQICFWVIAAARQIQKMRKFYFRRIMRMEIGWFDCNSVGELNTRFSDDINKINDAIADQMALFIQRMTSTICGFLLGFFRGWKLTLVIISVSPLIGIGAATIGLSVSKFTDYELKAYAKAGVVADEVISSMRTVAAFGGEKREVERYEKNLVFAQRWGIRKGIVMGFFTGFVWCLIFLCYALAFWYGSTLVLDEGEYTPGTLVQIFLSVIVGALNLGNASPCLEAFATGRAAATSIFETIDRKPIIDCMSEDGYKLDRIKGEIEFHNVTFHYPSRPEVKILNDLNMVIKPGEMTALVGPSGAGKSTALQLIQRFYDPCEGMVTVDGHDIRSLNIQWLRDQIGIVEQEPVLFSTTIAENIRYGREDATMEDIVQAAKEANAYNFIMDLPQQFDTLVGEGGGQMSGGQKQRVAIARALIRNPKILLLDMATSALDNESEAMVQEVLSKIQHGHTIISVAHRLSTVRAADTIIGFEHGTAVERGTHEELLERKGVYFTLVTLQSQGNQALNEEDIKDATEDDMLARTFSRGSYQDSLRASIRQRSKSQLSYLVHEPPLAVVDHKSTYEEDRKDKDIPVQEEVEPAPVRRILKFSAPEWPYMLVGSVGAAVNGTVTPLYAFLFSQILGTFSIPDKEEQRSQINGVCLLFVAMGCVSLFTQFLQGYAFAKSGELLTKRLRKFGFRAMLGQDIAWFDDLRNSPGALTTRLATDASQVQGAAGSQIGMIVNSFTNVTVAMIIAFSFSWKLSLVILCFFPFLALSGATQTRMLTGFASRDKQALEMVGQITNEALSNIRTVAGIGKERRFIEALETELEKPFKTAIQKANIYGFCFAFAQCIMFIANSASYRYGGYLISNEGLHFSYVFRVISAVVLSATALGRAFSYTPSYAKAKISAARFFQLLDRQPPISVYNTAGEKWDNFQGKIDFVDCKFTYPSRPDSQVLNGLSVSISPGQTLAFVGSSGCGKSTSIQLLERFYDPDQGKVMIDGHDSKKVNVQFLRSNIGIVSQEPVLFACSIMDNIKYGDNTKEIPMERVIAAAKQAQLHDFVMSLPEKYETNVGSQGSQLSRGEKQRIAIARAIVRDPKILLLDEATSALDTESEKTVQVALDKAREGRTCIVIAHRLSTIQNADIIAVMAQGVVIEKGTHEELMAQKGAYYKLVTTGSPIS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
90
Bovine
90
Sheep
90
Horse
80
Dog
80
Rabbit
80
Chicken
70
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O95342 作为底物

Site PTM Type Enzyme
S2 Phosphorylation
N109 N-Glycosylation
N116 N-Glycosylation
N122 N-Glycosylation
N125 N-Glycosylation
T586 Phosphorylation
S587 Phosphorylation
S593 Phosphorylation
S683 Phosphorylation
S686 Phosphorylation
S690 Phosphorylation
S694 Phosphorylation
S704 Phosphorylation
T858 Phosphorylation
T859 Phosphorylation
S1214 Phosphorylation
S1217 Phosphorylation

研究背景

功能:

Catalyzes the secretion of conjugated bile salts across the canalicular membrane of hepatocytes in an ATP-dependent manner. Transports taurine-conjugated bile salts more rapidly than glycine-conjugated bile salts.

细胞定位:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.

亚基结构:

Interacts with HAX1.

蛋白家族:

Multifunctional polypeptide with two homologous halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

研究领域

· Environmental Information Processing > Membrane transport > ABC transporters.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Organismal Systems > Digestive system > Cholesterol metabolism.

文献引用

1). Dolomiaea souliei ethyl acetate extract protected against α-naphthylisothiocyanate-induced acute intrahepatic cholestasis through regulation of farnesoid x receptor-mediated bile acid metabolism. Phytomedicine (PubMed: 34091148) [IF=7.9]

Application: WB    Species: Rat    Sample: liver tissue

Fig. 5. Effects of DSE on expressions of bile acid metabolism and inflammation related proteins in ANIT-induced cholestasis rats. + p < 0.01, vs. control; *p < 0.05, vs. model; **p < 0.01, vs. model.
2). Oleanolic acid alleviates ANIT-induced cholestatic liver injury by activating Fxr and Nrf2 pathways to ameliorate disordered bile acids homeostasis. PHYTOMEDICINE (PubMed: 35605478) [IF=7.9]
3). Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity. BIOCHEMICAL PHARMACOLOGY (PubMed: 31628911) [IF=5.8]
4). Variable p53/Nrf2 crosstalk contributes to triptolide-induced hepatotoxic process. Toxicology Letters (PubMed: 36990132) [IF=3.5]

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