| 产品: | ULK1 抗体 |
| 货号: | AF7648 |
| 描述: | Rabbit polyclonal antibody to ULK1 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 120kDa; 113kD(Calculated). |
| 蛋白号: | O75385 |
| RRID: | AB_2844012 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7648, RRID:AB_2844012.
展开/折叠
ATG 1; ATG1; ATG1 autophagy related 1 homolog; ATG1A; Autophagy related protein 1 homolog; Autophagy-related protein 1 homolog; FLJ38455; FLJ46475; hATG1; KIAA0722; Serine/threonine protein kinase ULK1; Serine/threonine protein kinase Unc51.1; Serine/threonine-protein kinase ULK1; ULK 1; ULK1; ULK1_HUMAN; Unc 51 (C. elegans) like kinase 1; UNC 51; Unc 51 like kinase 1; Unc-51 like kinase 1 (C. elegans); Unc-51-like kinase 1; UNC51; UNC51, C. elegans, homolog of; Unc51.1;
抗原和靶标
Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
- O75385 ULK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPRSSAIRRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGGRPYTPSPQVGTIPERPGWSGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLGCRLHSAPNLSDLHVVRPKLPKPPTDPLGAVFSPPQASPPQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPKTPSSQNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPFGRSFSTSRLTDLLLKAAFGTQAPDPGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSSPSPVVFTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAPAPELPAPGHGCSFADPITANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTLLFVQHVLEIAALKGSASEAAGGPEYQLQESVVADQISLLSREWGFAEQLVLYLKVAELLSSGLQSAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQSAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIERRLSALLTGICA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75385 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K13 | Ubiquitination | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| K162 | Acetylation | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| T180 | Phosphorylation | O75385 (ULK1) | Uniprot |
| S225 | Phosphorylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Q13131 (PRKAA1) , P54646 (PRKAA2) | Uniprot |
| S330 | Phosphorylation | Uniprot | |
| S403 | Phosphorylation | Uniprot | |
| S405 | Phosphorylation | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| T456 | Phosphorylation | Uniprot | |
| S459 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot | |
| T468 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | Uniprot | |
| S477 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| S522 | Phosphorylation | Uniprot | |
| R529 | Methylation | Uniprot | |
| S533 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| S539 | Phosphorylation | Uniprot | |
| S544 | Phosphorylation | Uniprot | |
| S556 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| S561 | Phosphorylation | Uniprot | |
| T575 | Phosphorylation | Uniprot | |
| S583 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| S593 | Phosphorylation | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| S605 | Phosphorylation | Uniprot | |
| K607 | Acetylation | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| T625 | Phosphorylation | Uniprot | |
| S630 | Phosphorylation | Uniprot | |
| T636 | Phosphorylation | Uniprot | |
| S638 | Phosphorylation | P42345 (MTOR) , P54646 (PRKAA2) , Q13131 (PRKAA1) | Uniprot |
| S639 | Phosphorylation | Uniprot | |
| T654 | Phosphorylation | Uniprot | |
| T660 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| S694 | Phosphorylation | Uniprot | |
| T695 | Phosphorylation | Uniprot | |
| T699 | Phosphorylation | Uniprot | |
| T709 | Phosphorylation | Uniprot | |
| S716 | Phosphorylation | Uniprot | |
| T717 | Phosphorylation | Uniprot | |
| S719 | Phosphorylation | Uniprot | |
| S748 | Phosphorylation | Uniprot | |
| S758 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S761 | Phosphorylation | Uniprot | |
| T764 | Phosphorylation | Uniprot | |
| S775 | Phosphorylation | Uniprot | |
| S781 | Phosphorylation | Uniprot | |
| S949 | Phosphorylation | Uniprot | |
| S954 | Phosphorylation | Uniprot | |
| S1042 | Phosphorylation | Uniprot | |
| T1046 | Phosphorylation | Uniprot |
翻译修饰 - O75385 作为激酶
| Substrate | Site | Source |
|---|---|---|
| A2RUS2 (DENND3) | S472 | Uniprot |
| A2RUS2 (DENND3) | S490 | Uniprot |
| O00560 (SDCBP) | S6 | Uniprot |
| O75143 (ATG13) | S355 | Uniprot |
| O75385 (ULK1) | T180 | Uniprot |
| Q13131 (PRKAA1) | S360 | Uniprot |
| Q13131 (PRKAA1) | T368 | Uniprot |
| Q13131 (PRKAA1) | S397 | Uniprot |
| Q13131 (PRKAA1) | S486 | Uniprot |
| Q13131 (PRKAA1) | T488 | Uniprot |
| Q7Z3C6 (ATG9A) | S761 | Uniprot |
| Q86WV6 (TMEM173) | S366 | Uniprot |
| Q8N122 (RPTOR) | S792 | Uniprot |
| Q8N122 (RPTOR) | S855 | Uniprot |
| Q8N122 (RPTOR) | S859 | Uniprot |
研究背景
Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy.
Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
Acetylated by KAT5/TIP60 under autophagy induction, promoting protein kinase activity.
Cytoplasm>Cytosol. Preautophagosomal structure.
Note: Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
Interacts with GABARAP and GABARAPL2. Interacts (via C-terminus) with ATG13. Part of a complex consisting of ATG13, ATG101, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation. Interacts with FEZ1; SCOC interferes with FEZ1-binding. Interacts with TBC1D14. Interacts (phosphorylated form) with TRIM5. When phosphorylated at Ser-317, interacts with MEFV and BECN1 simultaneously. Interacts with TRIM21 and IRF3, in the presence of TRIM21. Interacts with SESN2. Interacts with SQSTM1. Interacts with C9orf72. Interacts with WDR45.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.