IL-2RA/CD25 Antibody - #AF7675

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产品: IL-2RA/CD25 抗体
货号: AF7675
描述: Rabbit polyclonal antibody to IL-2RA/CD25
应用: WB IHC
反应: Human, Mouse
预测: Horse, Dog
分子量: 31kDa; 31kD(Calculated).
蛋白号: P01589
RRID: AB_2844039

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Horse(89%), Dog(86%)
克隆:
Polyclonal
特异性:
IL-2RA/CD25 Antibody detects endogenous levels of total IL-2RA/CD25.
RRID:
AB_2844039
引用格式: Affinity Biosciences Cat# AF7675, RRID:AB_2844039.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Interleukin 2 receptor alpha chain; CD25; CD25 antigen; IDDM10; IL 2 receptor alpha subunit; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2 RA; IL2-RA; IL2R; IL2R, alpha chain; IL2RA; IL2RA_HUMAN; IMD41; Interleukin 2 receptor alpha; Interleukin 2 receptor; Interleukin-2 receptor subunit alpha; p55; t-cell growth factor receptor; TAC; TAC antigen; TCGFR;

抗原和靶标

免疫原:
Uniprot:

P01589(IL2RA_HUMAN) >>Visit HPA database.

基因/基因ID:
IL2RA(3559)
序列:
MDSYLLMWGLLTFIMVPGCQAELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSCLVTTTDFQIQTEMAATMETSIFTTEYQVAVAGCVFLLISVLLLSGLTWQRRQRKSRRTI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
89
Dog
86
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P01589 作为底物

Site PTM Type Enzyme
N70 N-Glycosylation
N89 N-Glycosylation
T218 O-Glycosylation
T224 O-Glycosylation
T229 O-Glycosylation
T237 O-Glycosylation
S268 Phosphorylation P17252 (PRKCA)
T271 Phosphorylation P17252 (PRKCA)

研究背景

功能:

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

细胞定位:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

文献引用

1). Lactobacillus paracasei R3 protects against dextran sulfate sodium (DSS)-induced colitis in mice via regulating Th17/Treg cell balance. Journal of Translational Medicine, 2021 (PubMed: 34407839) [IF=7.4]

Application: WB    Species: Mouse    Sample: colon tissue

Fig. 6 L.p R3 attenuates colonic inflammatory responses but induces Treg responses in DSS-induced experimental murine colitis. A The expression of Foxp3, IL-10, IL-17A and IFN-γ were detected in the colon by the immunohistochemical method. B Pathological score of Foxp3, IL-10, IL-17A and IFN-γ expression in colon tissue. C Evaluate the expression of CD4, CD25, Foxp3, IL-17A, IFN-γ, IL-10 in colon tissue by Western blot. *p < 0.05; **p < 0.01; ***p < 0.001
2). Salvianolic acid B alleviates autoimmunity in Treg-deficient mice via inhibiting IL2-STAT5 signaling. Phytotherapy research : PTR, 2024 (PubMed: 38887974) [IF=7.2]
3). SLC35A2 expression drives breast cancer progression via ERK pathway activation. The FEBS journal, 2023 (PubMed: 38143314) [IF=5.4]

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