(3)
(6)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7681, RRID:AB_2844045.
展开/折叠
Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; C erb B2/neu protein; CD340; CD340 antigen; Cerb B2/neu protein; CerbB2; Erb b2 receptor tyrosine kinase 2; ERBB2; ERBB2_HUMAN; HER 2; HER 2/NEU; HER2; Herstatin; Human epidermal growth factor receptor 2; Metastatic lymph node gene 19 protein; MLN 19; MLN19; NEU; NEU proto oncogene; Neuro/glioblastoma derived oncogene homolog; Neuroblastoma/glioblastoma derived oncogene homolog; NGL; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; Receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase type cell surface receptor HER2; Tyrosine kinase-type cell surface receptor HER2; V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); V erb b2 avian erythroblastic leukemia viral oncogene homolog 2; V erb b2 avian erythroblastic leukemia viral oncoprotein 2; V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian);
抗原和靶标
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
- P04626 ERBB2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P04626 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| N68 | N-Glycosylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| K175 | Methylation | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| N259 | N-Glycosylation | Uniprot | |
| N530 | N-Glycosylation | Uniprot | |
| N571 | N-Glycosylation | Uniprot | |
| T686 | Phosphorylation | P17252 (PRKCA) , P17612 (PRKACA) , Q13237 (PRKG2) | Uniprot |
| T701 | Phosphorylation | Uniprot | |
| S703 | Phosphorylation | Uniprot | |
| K716 | Ubiquitination | Uniprot | |
| K724 | Ubiquitination | Uniprot | |
| S728 | Phosphorylation | Uniprot | |
| T733 | Phosphorylation | Uniprot | |
| Y735 | Phosphorylation | Uniprot | |
| K736 | Ubiquitination | Uniprot | |
| K747 | Ubiquitination | Uniprot | |
| K753 | Ubiquitination | Uniprot | |
| T759 | Phosphorylation | Uniprot | |
| Y772 | Phosphorylation | Uniprot | |
| S819 | Phosphorylation | Uniprot | |
| K854 | Ubiquitination | Uniprot | |
| K860 | Ubiquitination | Uniprot | |
| T875 | Phosphorylation | Uniprot | |
| Y877 | Phosphorylation | P04626 (ERBB2) | Uniprot |
| K883 | Ubiquitination | Uniprot | |
| K887 | Ubiquitination | Uniprot | |
| T900 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| Y923 | Phosphorylation | Uniprot | |
| K937 | Ubiquitination | Uniprot | |
| T948 | Phosphorylation | Uniprot | |
| S974 | Phosphorylation | Uniprot | |
| S977 | Phosphorylation | Uniprot | |
| S998 | Phosphorylation | Uniprot | |
| S1002 | Phosphorylation | Uniprot | |
| T1003 | Phosphorylation | Uniprot | |
| Y1005 | Phosphorylation | Uniprot | |
| S1007 | Phosphorylation | Uniprot | |
| Y1023 | Phosphorylation | P04626 (ERBB2) | Uniprot |
| S1049 | Phosphorylation | Uniprot | |
| S1050 | Phosphorylation | Uniprot | |
| S1051 | Phosphorylation | Uniprot | |
| T1052 | Phosphorylation | Uniprot | |
| S1054 | Phosphorylation | Uniprot | |
| T1060 | Phosphorylation | Uniprot | |
| S1066 | Phosphorylation | Uniprot | |
| S1073 | Phosphorylation | Uniprot | |
| S1078 | Phosphorylation | Uniprot | |
| S1083 | Phosphorylation | Uniprot | |
| S1100 | Phosphorylation | Uniprot | |
| T1103 | Phosphorylation | Uniprot | |
| S1107 | Phosphorylation | Uniprot | |
| Y1112 | Phosphorylation | P04626 (ERBB2) | Uniprot |
| S1113 | Phosphorylation | Uniprot | |
| S1122 | Phosphorylation | Uniprot | |
| T1124 | Phosphorylation | Uniprot | |
| Y1127 | Phosphorylation | Uniprot | |
| T1132 | Phosphorylation | Uniprot | |
| S1134 | Phosphorylation | Uniprot | |
| Y1139 | Phosphorylation | P04626 (ERBB2) | Uniprot |
| S1151 | Phosphorylation | Uniprot | |
| T1166 | Phosphorylation | Uniprot | |
| T1172 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| S1174 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| Y1196 | Phosphorylation | P04626 (ERBB2) | Uniprot |
| T1198 | Phosphorylation | Uniprot | |
| S1214 | Phosphorylation | Uniprot | |
| Y1221 | Phosphorylation | P04626 (ERBB2) | Uniprot |
| Y1222 | Phosphorylation | P04626 (ERBB2) | Uniprot |
| S1235 | Phosphorylation | Uniprot | |
| T1236 | Phosphorylation | Uniprot | |
| T1240 | Phosphorylation | Uniprot | |
| T1242 | Phosphorylation | Uniprot | |
| Y1248 | Phosphorylation | P00533 (EGFR) , P04626 (ERBB2) | Uniprot |
翻译修饰 - P04626 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O43157 (PLXNB1) | Y1708 | Uniprot |
| O43157 (PLXNB1) | Y1732 | Uniprot |
| P00338 (LDHA) | Y10 | Uniprot |
| P00533-1 (EGFR) | Y1069 | Uniprot |
| P04626 (ERBB2) | Y877 | Uniprot |
| P04626-5 (ERBB2) | Y993 | Uniprot |
| P04626 (ERBB2) | Y1023 | Uniprot |
| P04626-5 (ERBB2) | Y1109 | Uniprot |
| P04626 (ERBB2) | Y1112 | Uniprot |
| P04626 (ERBB2) | Y1139 | Uniprot |
| P04626-5 (ERBB2) | Y1191 | Uniprot |
| P04626-5 (ERBB2) | Y1192 | Uniprot |
| P04626 (ERBB2) | Y1196 | Uniprot |
| P04626-5 (ERBB2) | Y1218 | Uniprot |
| P04626-1 (ERBB2) | Y1221 | Uniprot |
| P04626 (ERBB2) | Y1222 | Uniprot |
| P04626 (ERBB2) | Y1248 | Uniprot |
| P06493 (CDK1) | Y15 | Uniprot |
| P35222 (CTNNB1) | Y654 | Uniprot |
| Q96N67 (DOCK7) | Y1257 | Uniprot |
| Q9BXH1 (BBC3) | Y58 | Uniprot |
| Q9BXH1 (BBC3) | Y152 | Uniprot |
| Q9BXH1 (BBC3) | Y172 | Uniprot |
研究背景
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues. Signaling via SEMA4C promotes phosphorylation at Tyr-1248. Dephosphorylated by PTPN12.
Cell membrane>Single-pass type I membrane protein. Early endosome. Cytoplasm>Perinuclear region. Nucleus.
Note: Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. Also detected in VPS35-positive endosome-to-TGN retrograde vesicles (PubMed:31138794).
Cytoplasm. Nucleus.
Cytoplasm. Nucleus.
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
Homodimer. Heterodimer with EGFR, ERBB3 and ERBB4. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with PLXNB1. Interacts (when phosphorylated on Tyr-1248) with MEMO1. Interacts with MUC1; the interaction is enhanced by heregulin (HRG). Interacts (when phosphorylated on Tyr-1139) with GRB7 (via SH2 domain). Interacts (when phosphorylated on Tyr-1248) with ERBIN. Interacts with KPNB1, RANBP2, EEA1, CRM1 and CLTC. Interacts with PTK6. Interacts with RPA194 and ACTB. Interacts with PRKCABP, SRC and MYOC (By similarity). Interacts (preferentially with the tyrosine phosphorylated form) with CPNE3; this interaction occurs at the cell membrane and is increased in a growth factor heregulin-dependent manner. Interacts with HSP90AA1 and HSP90AB1 in an ATP-dependent manner; the interaction suppresses ERBB2 kinase activity. Interacts with SORL1; this interaction regulates ERBB2 subcellular distribution by promoting its recycling after internalization from endosomes back to the plasma membrane, hence stimulates ERBB2-mediated signaling.
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Endometrial cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Bladder cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
文献引用
Application: WB Species: Human Sample: breast cancer
Application: IHC Species: Human Sample: BT474 cell
Application: WB Species: Mouse Sample: brain tissue
Application: IF/ICC Species: Mouse Sample: brain tissue
Application: WB Species: rat Sample: spinal dorsal horn
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