| 产品: | eIF2 alpha 抗体 |
| 货号: | AF7688 |
| 描述: | Rabbit polyclonal antibody to eIF2 alpha |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| 分子量: | 38kDa; 36kD(Calculated). |
| 蛋白号: | P05198 |
| RRID: | AB_2844052 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7688, RRID:AB_2844052.
展开/折叠
EIF 2 alpha; EIF 2; EIF 2A; EIF 2alpha; eIF-2-alpha; eIF-2A; EIF-2alpha; EIF2 alpha; EIF2; EIF2A; EIF2S1; Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa; Eukaryotic translation initiation factor 2 subunit 1 alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; IF2A_HUMAN;
抗原和靶标
- P05198 IF2A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLERENAEVDGDDDAEEMEAKAED
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P05198 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S5 | Phosphorylation | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Q9NZJ5 (EIF2AK3) , Q9BQI3 (EIF2AK1) , P19525 (EIF2AK2) , Q75MR0 (HRI) | Uniprot |
| S52 | Phosphorylation | B4DS64 (PRKRIR) , P51812 (RPS6KA3) , P19525 (EIF2AK2) , P28482 (MAPK1) , Q75MR0 (HRI) , Q9BQI3 (EIF2AK1) , Q9NZJ5 (EIF2AK3) , Q05655 (PRKCD) , Q16539 (MAPK14) , Q9P2K8 (EIF2AK4) | Uniprot |
| S58 | Phosphorylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K101 | Acetylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| R133 | Methylation | Uniprot | |
| K141 | Acetylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K143 | Acetylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| Y147 | Phosphorylation | Uniprot | |
| Y150 | Phosphorylation | Uniprot | |
| K154 | Methylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| T185 | Phosphorylation | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| Y200 | Phosphorylation | Uniprot | |
| Y202 | Phosphorylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| Y235 | Phosphorylation | Uniprot | |
| T245 | Phosphorylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| R266 | Methylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| T279 | Phosphorylation | Uniprot | |
| T281 | Phosphorylation | Uniprot | |
| T284 | Phosphorylation | Uniprot | |
| K312 | Ubiquitination | Uniprot |
研究背景
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation (By similarity).
Cytoplasm>Stress granule.
Note: Colocalizes with NANOS3 in the stress granules.
Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interaction with METAP2 protects EIF2S1 from inhibitory phosphorylation (By similarity). Interacts with ABCF1 isoform 2. Associates with ribosomes. Interacts with DDX3X in an RNA-independent manner.
Belongs to the eIF-2-alpha family.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Genetic Information Processing > Translation > RNA transport.
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
限制条款
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