| 产品: | Cytokeratin 18 抗体 |
| 货号: | AF7692 |
| 描述: | Rabbit polyclonal antibody to Cytokeratin 18 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Rabbit |
| 分子量: | 46kDa; 48kD(Calculated). |
| 蛋白号: | P05783 |
| RRID: | AB_2844056 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7692, RRID:AB_2844056.
展开/折叠
Cell proliferation inducing gene 46 protein; Cell proliferation inducing protein 46; Cell proliferation-inducing gene 46 protein; CK 18; CK-18; CK18; CYK 18; CYK18; Cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; K 18; K18; K1C18_HUMAN; KA18; Keratin 18; Keratin 18, type I; Keratin D; keratin, type I cytoskeletal 18; Keratin-18; Krt18;
抗原和靶标
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
- P05783 K1C18_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSFTTRSTFSTNYRSLGSVQAPSYGARPVSSAASVYAGAGGSGSRISVSRSTSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRLASYLDRVRSLETENRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P05783 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| T4 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| T11 | Phosphorylation | Uniprot | |
| Y13 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| Y24 | Phosphorylation | Uniprot | |
| R27 | Methylation | Uniprot | |
| S30 | O-Glycosylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| S31 | O-Glycosylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | P06493 (CDK1) | Uniprot |
| Y36 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| R45 | Methylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| S49 | O-Glycosylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| R50 | Methylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| T52 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | P17252 (PRKCA) , Q9UBS0 (RPS6KB2) , P49137 (MAPKAPK2) , Q02156 (PRKCE) , P51812 (RPS6KA3) , Q14012 (CAMK1) , Q9UQM7 (CAMK2A) | Uniprot |
| R55 | Methylation | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| T65 | Phosphorylation | Uniprot | |
| K81 | Acetylation | Uniprot | |
| K81 | Sumoylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| T83 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| R90 | Methylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| Y94 | Phosphorylation | Uniprot | |
| R97 | Methylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| K111 | Acetylation | Uniprot | |
| K118 | Methylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| Y129 | Phosphorylation | Uniprot | |
| K131 | Acetylation | Uniprot | |
| K131 | Methylation | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| K167 | Acetylation | Uniprot | |
| K167 | Methylation | Uniprot | |
| K167 | Sumoylation | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| Y168 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| K187 | Acetylation | Uniprot | |
| K187 | Methylation | Uniprot | |
| K187 | Sumoylation | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| T192 | Phosphorylation | Uniprot | |
| T195 | Phosphorylation | Uniprot | |
| T201 | Phosphorylation | Uniprot | |
| K207 | Sumoylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K214 | Acetylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K222 | Acetylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| K247 | Acetylation | Uniprot | |
| K247 | Sumoylation | Uniprot | |
| K247 | Ubiquitination | Uniprot | |
| Y256 | Phosphorylation | Uniprot | |
| K262 | Ubiquitination | Uniprot | |
| K269 | Sumoylation | Uniprot | |
| Y270 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| T302 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| K317 | Acetylation | Uniprot | |
| K317 | Sumoylation | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| Y331 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| Y363 | Phosphorylation | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| K372 | Sumoylation | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| T379 | Phosphorylation | Uniprot | |
| Y380 | Phosphorylation | Uniprot | |
| S398 | Phosphorylation | Uniprot | |
| S399 | Phosphorylation | Uniprot | |
| S401 | Phosphorylation | Uniprot | |
| T404 | Phosphorylation | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| T409 | Phosphorylation | Uniprot | |
| K417 | Acetylation | Uniprot | |
| K417 | Sumoylation | Uniprot | |
| K417 | Ubiquitination | Uniprot | |
| S420 | Phosphorylation | Uniprot | |
| T422 | Phosphorylation | Uniprot | |
| T425 | Phosphorylation | Uniprot | |
| K426 | Acetylation | Uniprot | |
| K426 | Methylation | Uniprot | |
| K426 | Sumoylation | Uniprot | |
| K426 | Ubiquitination | Uniprot |
研究背景
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
Cytoplasm>Perinuclear region. Nucleus>Nucleolus.
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Heterotetramer of two type I and two type II keratins. KRT18 associates with KRT8. Interacts with the thrombin-antithrombin complex (By similarity). Interacts with PNN and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with DNAJB6, TCHP and TRADD. Interacts with FAM83H. Interacts with EPPK1 (By similarity).
(Microbial infection) Interacts with hepatitis C virus/HCV core protein.
Belongs to the intermediate filament family.
研究领域
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)
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