| 产品: | Bcr 抗体 |
| 货号: | AF7715 |
| 描述: | Rabbit polyclonal antibody to Bcr |
| 应用: | WB |
| 反应: | Human, Mouse, Monkey |
| 预测: | Horse, Rabbit, Dog |
| 分子量: | 160kDa; 143kD(Calculated). |
| 蛋白号: | P11274 |
| RRID: | AB_2844079 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7715, RRID:AB_2844079.
展开/折叠
ALL; bcr; BCR/ABL FUSION GENE, INCLUDED; BCR/FGFR1 chimera protein; BCR/FGFR1 FUSION GENE, INCLUDED; BCR/PDGFRA FUSION GENE, INCLUDED; BCR_HUMAN; BCR1; Breakpoint cluster region; Breakpoint cluster region protein; CML; D22S11; D22S662; FGFR1/BCR chimera protein; PHL; Renal carcinoma antigen NY-REN-26;
抗原和靶标
- P11274 BCR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVDPVGFAEAWKAQFPDSEPPRMELRSVGDIEQELERCKASIRRLEQEVNQERFRMIYLQTLLAKEKKSYDRQRWGFRRAAQAPDGASEPRASASRPQPAPADGADPPPAEEPEARPDGEGSPGKARPGTARRPGAAASGERDDRGPPASVAALRSNFERIRKGHGQPGADAEKPFYVNVEFHHERGLVKVNDKEVSDRISSLGSQAMQMERKKSQHGAGSSVGDASRPPYRGRSSESSCGVDGDYEDAELNPRFLKDNLIDANGGSRPPWPPLEYQPYQSIYVGGMMEGEGKGPLLRSQSTSEQEKRLTWPRRSYSPRSFEDCGGGYTPDCSSNENLTSSEEDFSSGQSSRVSPSPTTYRMFRDKSRSPSQNSQQSFDSSSPPTPQCHKRHRHCPVVVSEATIVGVRKTGQIWPNDGEGAFHGDADGSFGTPPGYGCAADRAEEQRRHQDGLPYIDDSPSSSPHLSSKGRGSRDALVSGALESTKASELDLEKGLEMRKWVLSGILASEETYLSHLEALLLPMKPLKAAATTSQPVLTSQQIETIFFKVPELYEIHKEFYDGLFPRVQQWSHQQRVGDLFQKLASQLGVYRAFVDNYGVAMEMAEKCCQANAQFAEISENLRARSNKDAKDPTTKNSLETLLYKPVDRVTRSTLVLHDLLKHTPASHPDHPLLQDALRISQNFLSSINEEITPRRQSMTVKKGEHRQLLKDSFMVELVEGARKLRHVFLFTDLLLCTKLKKQSGGKTQQYDCKWYIPLTDLSFQMVDELEAVPNIPLVPDEELDALKIKISQIKNDIQREKRANKGSKATERLKKKLSEQESLLLLMSPSMAFRVHSRNGKSYTFLISSDYERAEWRENIREQQKKCFRSFSLTSVELQMLTNSCVKLQTVHSIPLTINKEDDESPGLYGFLNVIVHSATGFKQSSNLYCTLEVDSFGYFVNKAKTRVYRDTAEPNWNEEFEIELEGSQTLRILCYEKCYNKTKIPKEDGESTDRLMGKGQVQLDPQALQDRDWQRTVIAMNGIEVKLSVKFNSREFSLKRMPSRKQTGVFGVKIAVVTKRERSKVPYIVRQCVEEIERRGMEEVGIYRVSGVATDIQALKAAFDVNNKDVSVMMSEMDVNAIAGTLKLYFRELPEPLFTDEFYPNFAEGIALSDPVAKESCMLNLLLSLPEANLLTFLFLLDHLKRVAEKEAVNKMSLHNLATVFGPTLLRPSEKESKLPANPSQPITMTDSWSLEVMSQVQVLLYFLQLEAIPAPDSKRQSILFSTEV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P11274 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| Y58 | Phosphorylation | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| T130 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| Y177 | Phosphorylation | P06241 (FYN) , A9UF07 (BCR/ABL fusion) , P07332 (FES) , P08631 (HCK) , P00519 (ABL1) , A0A173G4P4 (Abl fusion) , P07948 (LYN) | Uniprot |
| K194 | Acetylation | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| K213 | Acetylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| Y231 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| C240 | S-Nitrosylation | Uniprot | |
| Y246 | Phosphorylation | P07332 (FES) | Uniprot |
| S267 | Phosphorylation | Uniprot | |
| Y276 | Phosphorylation | P07332 (FES) | Uniprot |
| Y279 | Phosphorylation | P07332 (FES) | Uniprot |
| S281 | Phosphorylation | Uniprot | |
| Y283 | Phosphorylation | A9UF07 (BCR/ABL fusion) , P07332 (FES) | Uniprot |
| S299 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| T302 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| T310 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| Y328 | Phosphorylation | A9UF07 (BCR/ABL fusion) | Uniprot |
| T329 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| T358 | Phosphorylation | Uniprot | |
| T359 | Phosphorylation | Uniprot | |
| Y360 | Phosphorylation | A9UF07 (BCR/ABL fusion) , A0A173G4P4 (Abl fusion) | Uniprot |
| S374 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| T385 | Phosphorylation | Uniprot | |
| T410 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| Y436 | Phosphorylation | Uniprot | |
| Y455 | Phosphorylation | Uniprot | |
| S459 | Phosphorylation | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| S462 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot | |
| S468 | Phosphorylation | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| K486 | Ubiquitination | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| K494 | Ubiquitination | Uniprot | |
| Y513 | Phosphorylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| K549 | Ubiquitination | Uniprot | |
| Y554 | Phosphorylation | Uniprot | |
| K558 | Ubiquitination | Uniprot | |
| Y561 | Phosphorylation | Uniprot | |
| S572 | Phosphorylation | Uniprot | |
| K583 | Ubiquitination | Uniprot | |
| S586 | Phosphorylation | Uniprot | |
| Y591 | Phosphorylation | Uniprot | |
| Y598 | Phosphorylation | Uniprot | |
| S626 | Phosphorylation | Uniprot | |
| T634 | Phosphorylation | Uniprot | |
| T635 | Phosphorylation | Uniprot | |
| K636 | Ubiquitination | Uniprot | |
| S638 | Phosphorylation | Uniprot | |
| T641 | Phosphorylation | Uniprot | |
| Y644 | Phosphorylation | Uniprot | |
| K645 | Ubiquitination | Uniprot | |
| T651 | Phosphorylation | Uniprot | |
| S653 | Phosphorylation | Uniprot | |
| T654 | Phosphorylation | Uniprot | |
| K662 | Ubiquitination | Uniprot | |
| T693 | Phosphorylation | Uniprot | |
| S698 | Phosphorylation | Uniprot | |
| Y751 | Phosphorylation | Uniprot | |
| Y756 | Phosphorylation | Uniprot | |
| K795 | Ubiquitination | Uniprot | |
| S843 | Phosphorylation | Uniprot | |
| Y844 | Phosphorylation | Uniprot | |
| T845 | Phosphorylation | Uniprot | |
| Y852 | Phosphorylation | Uniprot | |
| S871 | Phosphorylation | Uniprot | |
| S894 | Phosphorylation | Uniprot | |
| T898 | Phosphorylation | Uniprot | |
| S906 | Phosphorylation | Uniprot | |
| Y910 | Phosphorylation | Uniprot | |
| S919 | Phosphorylation | Uniprot | |
| T921 | Phosphorylation | Uniprot | |
| K1000 | Ubiquitination | Uniprot | |
| T1018 | Phosphorylation | Uniprot | |
| S1030 | Phosphorylation | Uniprot | |
| S1035 | Phosphorylation | Uniprot | |
| S1039 | Phosphorylation | Uniprot | |
| K1047 | Ubiquitination | Uniprot | |
| T1049 | Phosphorylation | Uniprot | |
| K1061 | Ubiquitination | Uniprot | |
| K1066 | Ubiquitination | Uniprot | |
| Y1069 | Phosphorylation | Uniprot | |
| Y1089 | Phosphorylation | Uniprot | |
| K1102 | Ubiquitination | Uniprot | |
| K1197 | Ubiquitination | Uniprot | |
| S1264 | Phosphorylation | Uniprot |
翻译修饰 - P11274 作为激酶
研究背景
Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form. The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form. The amino terminus contains an intrinsic kinase activity. Functions as an important negative regulator of neuronal RAC1 activity (By similarity). Regulates macrophage functions such as CSF1-directed motility and phagocytosis through the modulation of RAC1 activity. Plays a major role as a RHOA GEF in keratinocytes being involved in focal adhesion formation and keratinocyte differentiation.
Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
Cell junction>Synapse>Postsynaptic density. Cell projection>Dendritic spine. Cell projection>Axon. Cell junction>Synapse.
Homotetramer. Interacts with PDZK1. May interact with CCPG1 (By similarity). Interacts with FES/FPS, ABL1, PIK3R1 and GRB2. Interacts with HCK. Interacts with SH2D5. Interacts with DLG4.
The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.
The DH domain is involved in interaction with CCPG1.
The amino terminus contains an intrinsic kinase activity. The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form. The C-terminus is a Rho-GAP domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. The protein has a unique structure having two opposing regulatory activities toward small GTP-binding proteins.
研究领域
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)
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