产品: EEF2 抗体
货号: AF7720
描述: Rabbit polyclonal antibody to EEF2
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog, Chicken
分子量: 95kDa; 95kD(Calculated).
蛋白号: P13639
RRID: AB_2844084

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
EEF2 Antibody detects endogenous levels of total EEF2.
RRID:
AB_2844084
引用格式: Affinity Biosciences Cat# AF7720, RRID:AB_2844084.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EEF 2; Eef2; EF-2; EF2; EF2_HUMAN; Elongation factor 2; Eukaryotic translation elongation factor 2; Polypeptidyl tRNA translocase; SCA26;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGAGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGPAERAKKVEDMMKKLWGDRYFDPANGKFSKSATSPEGKKLPRTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P13639 作为底物

Site PTM Type Enzyme
R10 Methylation
K15 Ubiquitination
S23 Phosphorylation
K32 Ubiquitination
S38 Phosphorylation
C41 S-Nitrosylation
K42 Acetylation
K42 Ubiquitination
S48 Phosphorylation
R50 Methylation
T54 Phosphorylation O00418 (EEF2K)
T57 Phosphorylation O00418 (EEF2K)
T59 Phosphorylation O00418 (EEF2K)
C67 S-Nitrosylation
S72 Phosphorylation
T73 Phosphorylation
S76 Phosphorylation
Y79 Phosphorylation
K90 Ubiquitination
K93 Ubiquitination
K152 Ubiquitination
K159 Ubiquitination
Y175 Phosphorylation
T177 Phosphorylation
S197 Phosphorylation
K235 Acetylation
K235 Ubiquitination
K239 Acetylation
K239 Sumoylation
K239 Ubiquitination
K251 Ubiquitination
K252 Acetylation
K252 Ubiquitination
K258 Acetylation
K259 Ubiquitination
R264 Methylation
Y265 Phosphorylation
K272 Acetylation
K272 Methylation
K272 Ubiquitination
S274 Phosphorylation
K275 Acetylation
K275 Ubiquitination
K283 Ubiquitination
T288 Phosphorylation
C290 S-Nitrosylation
K308 Acetylation
K308 Ubiquitination
K314 Acetylation
K314 Ubiquitination
K318 Acetylation
K318 Ubiquitination
K322 Acetylation
K322 Sumoylation
K322 Ubiquitination
S325 Phosphorylation
K330 Ubiquitination
K333 Ubiquitination
K337 Acetylation
K337 Ubiquitination
T355 Phosphorylation
S360 Phosphorylation
K366 Acetylation
K366 Ubiquitination
C369 S-Nitrosylation
Y373 Phosphorylation
K386 Ubiquitination
K391 Acetylation
K391 Ubiquitination
S399 Phosphorylation
K400 Ubiquitination
T404 Phosphorylation
K407 Acetylation
K407 Methylation
K407 Ubiquitination
R409 Methylation
Y411 Phosphorylation
S418 Phosphorylation
K426 Acetylation
K426 Ubiquitination
R428 Methylation
Y434 Phosphorylation
T435 Phosphorylation P24941 (CDK2)
K438 Acetylation
K438 Ubiquitination
K439 Acetylation
K439 Ubiquitination
Y443 Phosphorylation
K445 Acetylation
K445 Methylation
K445 Ubiquitination
T450 Phosphorylation
K481 Ubiquitination
R495 Methylation
K498 Acetylation
K498 Methylation
K498 Ubiquitination
S500 Phosphorylation
S502 Phosphorylation
K512 Ubiquitination
K519 Methylation
K519 Ubiquitination
K525 Methylation
K525 Ubiquitination
R526 Methylation
K529 Sumoylation
S530 Phosphorylation
S541 Phosphorylation
C567 S-Nitrosylation
K571 Ubiquitination
K572 Ubiquitination
Y579 Phosphorylation
T582 Phosphorylation
S584 Phosphorylation
S587 Phosphorylation
C591 S-Nitrosylation
S593 Phosphorylation
K594 Methylation
K594 Ubiquitination
S595 Phosphorylation P24941 (CDK2)
K598 Ubiquitination
K605 Ubiquitination
K619 Ubiquitination
S623 Phosphorylation
K629 Ubiquitination
K638 Acetylation
K638 Methylation
K638 Ubiquitination
K648 Acetylation
K648 Ubiquitination
C651 S-Nitrosylation
K667 Ubiquitination
Y671 Phosphorylation
K676 Ubiquitination
S678 Phosphorylation
K688 Acetylation
K688 Ubiquitination
R698 Methylation
R716 Methylation
T724 Phosphorylation
R726 Methylation
C728 S-Nitrosylation
Y730 Phosphorylation
S732 Phosphorylation
R739 Methylation
Y745 Phosphorylation
Y760 Phosphorylation
K766 Ubiquitination
R767 Methylation
T779 Phosphorylation
S793 Phosphorylation
S802 Phosphorylation
K842 Ubiquitination
K845 Ubiquitination
K857 Acetylation
K857 Ubiquitination

研究背景

功能:

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

翻译修饰:

Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment.

Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine (By similarity).

(Microbial infection) Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.

ISGylated.

Proteolytically processed at two sites following phosphorylation by CSK.

SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage.

细胞定位:

Cytoplasm. Nucleus.
Note: Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9. Interacts with RBPMS2.

蛋白家族:

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.