EEF2 Antibody | Affinity Biosciences LTD|亲科生物官网

WB
IF/ICC
Cites

1/4

Western blot analysis of extracts from PC12, using eEF2 Antibody.

AF7721 staining Hela cells by IF/ICC.

Fig.

产品:	EEF2 抗体
货号:	AF7721
描述:	Rabbit polyclonal antibody to EEF2
应用:	WB IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Horse, Sheep, Dog, Chicken
分子量:	95kDa; 95kD(Calculated).
蛋白号:	P13639
RRID:	AB_2844085

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)

克隆:

Polyclonal

特异性:

EEF2 Antibody detects endogenous levels of total EEF2.

RRID:

AB_2844085
引用格式: Affinity Biosciences Cat# AF7721, RRID:AB_2844085.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

EEF 2; Eef2; EF-2; EF2; EF2_HUMAN; Elongation factor 2; Eukaryotic translation elongation factor 2; Polypeptidyl tRNA translocase; SCA26;

抗原和靶标

免疫原:

Uniprot:

P13639(EF2_HUMAN) >>Visit HPA database.

基因/基因ID:

EEF2(1938)

序列:

P13639 EF2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGAGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGPAERAKKVEDMMKKLWGDRYFDPANGKFSKSATSPEGKKLPRTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P13639 作为底物

P13639

Site	PTM Type	Enzyme	Source
R10	Methylation		Uniprot
K15	Ubiquitination		Uniprot
S23	Phosphorylation		Uniprot
K32	Ubiquitination		Uniprot
S38	Phosphorylation		Uniprot
C41	S-Nitrosylation		Uniprot
K42	Acetylation		Uniprot
K42	Ubiquitination		Uniprot
S48	Phosphorylation		Uniprot
R50	Methylation		Uniprot
T54	Phosphorylation	O00418 (EEF2K)	Uniprot
T57	Phosphorylation	O00418 (EEF2K)	Uniprot
T59	Phosphorylation	O00418 (EEF2K)	Uniprot
C67	S-Nitrosylation		Uniprot
S72	Phosphorylation		Uniprot
T73	Phosphorylation		Uniprot
S76	Phosphorylation		Uniprot
Y79	Phosphorylation		Uniprot
K90	Ubiquitination		Uniprot
K93	Ubiquitination		Uniprot
K152	Ubiquitination		Uniprot
K159	Ubiquitination		Uniprot
Y175	Phosphorylation		Uniprot
T177	Phosphorylation		Uniprot
S197	Phosphorylation		Uniprot
K235	Acetylation		Uniprot
K235	Ubiquitination		Uniprot
K239	Acetylation		Uniprot
K239	Sumoylation		Uniprot
K239	Ubiquitination		Uniprot
K251	Ubiquitination		Uniprot
K252	Acetylation		Uniprot
K252	Ubiquitination		Uniprot
K258	Acetylation		Uniprot
K259	Ubiquitination		Uniprot
R264	Methylation		Uniprot
Y265	Phosphorylation		Uniprot
K272	Acetylation		Uniprot
K272	Methylation		Uniprot
K272	Ubiquitination		Uniprot
S274	Phosphorylation		Uniprot
K275	Acetylation		Uniprot
K275	Ubiquitination		Uniprot
K283	Ubiquitination		Uniprot
T288	Phosphorylation		Uniprot
C290	S-Nitrosylation		Uniprot
K308	Acetylation		Uniprot
K308	Ubiquitination		Uniprot
K314	Acetylation		Uniprot
K314	Ubiquitination		Uniprot
K318	Acetylation		Uniprot
K318	Ubiquitination		Uniprot
K322	Acetylation		Uniprot
K322	Sumoylation		Uniprot
K322	Ubiquitination		Uniprot
S325	Phosphorylation		Uniprot
K330	Ubiquitination		Uniprot
K333	Ubiquitination		Uniprot
K337	Acetylation		Uniprot
K337	Ubiquitination		Uniprot
T355	Phosphorylation		Uniprot
S360	Phosphorylation		Uniprot
K366	Acetylation		Uniprot
K366	Ubiquitination		Uniprot
C369	S-Nitrosylation		Uniprot
Y373	Phosphorylation		Uniprot
K386	Ubiquitination		Uniprot
K391	Acetylation		Uniprot
K391	Ubiquitination		Uniprot
S399	Phosphorylation		Uniprot
K400	Ubiquitination		Uniprot
T404	Phosphorylation		Uniprot
K407	Acetylation		Uniprot
K407	Methylation		Uniprot
K407	Ubiquitination		Uniprot
R409	Methylation		Uniprot
Y411	Phosphorylation		Uniprot
S418	Phosphorylation		Uniprot
K426	Acetylation		Uniprot
K426	Ubiquitination		Uniprot
R428	Methylation		Uniprot
Y434	Phosphorylation		Uniprot
T435	Phosphorylation	P24941 (CDK2)	Uniprot
K438	Acetylation		Uniprot
K438	Ubiquitination		Uniprot
K439	Acetylation		Uniprot
K439	Ubiquitination		Uniprot
Y443	Phosphorylation		Uniprot
K445	Acetylation		Uniprot
K445	Methylation		Uniprot
K445	Ubiquitination		Uniprot
T450	Phosphorylation		Uniprot
K481	Ubiquitination		Uniprot
R495	Methylation		Uniprot
K498	Acetylation		Uniprot
K498	Methylation		Uniprot
K498	Ubiquitination		Uniprot
S500	Phosphorylation		Uniprot
S502	Phosphorylation		Uniprot
K512	Ubiquitination		Uniprot
K519	Methylation		Uniprot
K519	Ubiquitination		Uniprot
K525	Methylation		Uniprot
K525	Ubiquitination		Uniprot
R526	Methylation		Uniprot
K529	Sumoylation		Uniprot
S530	Phosphorylation		Uniprot
S541	Phosphorylation		Uniprot
C567	S-Nitrosylation		Uniprot
K571	Ubiquitination		Uniprot
K572	Ubiquitination		Uniprot
Y579	Phosphorylation		Uniprot
T582	Phosphorylation		Uniprot
S584	Phosphorylation		Uniprot
S587	Phosphorylation		Uniprot
C591	S-Nitrosylation		Uniprot
S593	Phosphorylation		Uniprot
K594	Methylation		Uniprot
K594	Ubiquitination		Uniprot
S595	Phosphorylation	P24941 (CDK2)	Uniprot
K598	Ubiquitination		Uniprot
K605	Ubiquitination		Uniprot
K619	Ubiquitination		Uniprot
S623	Phosphorylation		Uniprot
K629	Ubiquitination		Uniprot
K638	Acetylation		Uniprot
K638	Methylation		Uniprot
K638	Ubiquitination		Uniprot
K648	Acetylation		Uniprot
K648	Ubiquitination		Uniprot
C651	S-Nitrosylation		Uniprot
K667	Ubiquitination		Uniprot
Y671	Phosphorylation		Uniprot
K676	Ubiquitination		Uniprot
S678	Phosphorylation		Uniprot
K688	Acetylation		Uniprot
K688	Ubiquitination		Uniprot
R698	Methylation		Uniprot
R716	Methylation		Uniprot
T724	Phosphorylation		Uniprot
R726	Methylation		Uniprot
C728	S-Nitrosylation		Uniprot
Y730	Phosphorylation		Uniprot
S732	Phosphorylation		Uniprot
R739	Methylation		Uniprot
Y745	Phosphorylation		Uniprot
Y760	Phosphorylation		Uniprot
K766	Ubiquitination		Uniprot
R767	Methylation		Uniprot
T779	Phosphorylation		Uniprot
S793	Phosphorylation		Uniprot
S802	Phosphorylation		Uniprot
K842	Ubiquitination		Uniprot
K845	Ubiquitination		Uniprot
K857	Acetylation		Uniprot
K857	Ubiquitination		Uniprot

研究背景

P13639_EF2_HUMAN

功能:

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

翻译修饰:

Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment.

Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine (By similarity).

(Microbial infection) Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.

ISGylated.

Proteolytically processed at two sites following phosphorylation by CSK.

SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage.

细胞定位:

Cytoplasm. Nucleus.
Note: Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product.

亚基结构:

Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9. Interacts with RBPMS2.

蛋白家族:

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

文献引用

1). Myostatin inhibits eEF2K-eEF2 by regulating AMPK to suppress protein synthesis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2017 (PubMed: 29024627) [IF=3.1]

Application: WB Species: mouse Sample:

Fig. 3. Effect of myostatin on translation elongation pathway. C2C12 myotubes (4–5 days post-differentiation) were treated with various concentration recombinant myostatin (0, 0.01, 0.1, 1, 2, 3 µg/ml) for 48 h and then stimulated with 1 µg/mL puromycin for 30 min. Cells were then lysed and cellular extracts were analyzed by Western blot (ABC).

Application: WB Species: mouse Sample: C2C12 myotubes

Fig. 3. |Effect of myostatin on translation elongation pathway.C2C12 myotubes (4–5 days post-differentiation) were treated with various concentration recombinant myostatin (0, 0.01, 0.1, 1, 2, 3 µg/ml) for 48 h and then stimulated with 1 µg/mL puromycin for 30 min. Cells were then lysed and cellular extracts were analyzed by Western blot

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EEF2 Antibody - #AF7721

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