产品: ATF1 抗体
货号: AF7752
描述: Rabbit polyclonal antibody to ATF1
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 29kDa; 29kD(Calculated).
蛋白号: P18846
RRID: AB_2844116

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(89%)
克隆:
Polyclonal
特异性:
ATF1 Antibody detects endogenous levels of total ATF1.
RRID:
AB_2844116
引用格式: Affinity Biosciences Cat# AF7752, RRID:AB_2844116.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Activating transcription factor 1; ATF 1; atf1; ATF1 EWS fusion gene; ATF1 FUS fusion gene; ATF1_HUMAN; cAMP dependent transcription factor 1; cAMP-dependent transcription factor ATF-1; Cyclic AMP dependent transcription factor ATF 1; Cyclic AMP dependent transcription factor ATF1; Cyclic AMP-dependent transcription factor ATF-1; EWS AFT1; FUS ATF 1; FUS/ATF 1; Protein TREB36; RNA binding protein activating transcription factor 1 fusion protein; TREB 36; TREB36; TREB36 protein;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSV

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
89
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P18846 作为底物

Site PTM Type Enzyme
S10 Phosphorylation
S36 Phosphorylation P68400 (CSNK2A1) , P48729 (CSNK1A1)
S38 Phosphorylation P68400 (CSNK2A1)
S41 Phosphorylation P48729 (CSNK1A1) , P68400 (CSNK2A1)
S44 Phosphorylation P68400 (CSNK2A1)
S47 Phosphorylation P48729 (CSNK1A1) , P68400 (CSNK2A1)
S50 Phosphorylation P68400 (CSNK2A1) , P48729 (CSNK1A1)
S51 Phosphorylation P48729 (CSNK1A1) , P68400 (CSNK2A1)
S63 Phosphorylation O75676 (RPS6KA4) , Q00526 (CDK3) , Q14012 (CAMK1) , O75582 (RPS6KA5) , P68400 (CSNK2A1) , P51812 (RPS6KA3)
Y64 Phosphorylation
K69 Acetylation
K69 Ubiquitination
S73 Phosphorylation
T183 Phosphorylation
T184 Phosphorylation
S186 Phosphorylation
T188 Phosphorylation
S189 O-Glycosylation
T197 Phosphorylation
S198 Phosphorylation Q9H2X6 (HIPK2)
T209 Phosphorylation
K215 Ubiquitination
K239 Ubiquitination
R244 Methylation
K253 Ubiquitination
K260 Ubiquitination
K263 Ubiquitination
S267 Phosphorylation
S270 Phosphorylation

研究背景

功能:

This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.

翻译修饰:

Phosphorylated at Ser-198 by HIPK2 in response to genotoxic stress. This phosphorylation promotes transcription repression of FTH1 and other antioxidant detoxification genes. The CDK3-mediated phosphorylation at Ser-63 promotes its transactivation and transcriptional activities. Phosphorylated at Ser-63 by RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Binds DNA as a dimer. Interacts with HIPK2 and CDK3.

蛋白家族:

Belongs to the bZIP family. ATF subfamily.

研究领域

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

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