产品: CDKN2A/p16INK4a 抗体
货号: AF0228
描述: Rabbit polyclonal antibody to CDKN2A/p16INK4a
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Rabbit
分子量: 16kDa; 17kD(Calculated).
蛋白号: P42771
RRID: AB_2833403

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Rabbit(100%)
克隆:
Polyclonal
特异性:
CDKN2A/p16INK4a Antibody detects endogenous levels of total CDKN2A/p16INK4a.
RRID:
AB_2833403
引用格式: Affinity Biosciences Cat# AF0228, RRID:AB_2833403.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cyclin-dependent kinase inhibitor 2A;Cyclin-dependent kinase 4 inhibitor A;CDK4I;Multiple tumor suppressor 1;MTS-1;p16-INK4a;p16-INK4;p16INK4A;CDKN2A;CDKN2;MTS1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P42771 CDN2A_HUMAN:

Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific.

描述:
p16-INK4A a cell-cycle regulatory protein that Interacts with CDK4 and CDK6, inhibiting their ability to interact with cyclins D. Inhibits the phosphorylation of the retinoblastoma protein by CDK4 or CDK6. Four alternatively spliced isoforms have been reported.
序列:
MEPAAGSSMEPSADWLATAAARGRVEEVRALLEAGALPNAPNSYGRRPIQVMMMGSARVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEELGHRDVARYLRAAAGGTRGSNHARIDAAEGPSDIPD

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Rabbit
100
Horse
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P42771 作为底物

Site PTM Type Enzyme
M1 Acetylation
S7 Phosphorylation Q13535 (ATR)
S8 Phosphorylation O14920 (IKBKB) , Q13535 (ATR)
Y44 Phosphorylation
R99 Methylation
R131 Methylation
R138 Methylation
S140 Phosphorylation Q13535 (ATR)
S152 Phosphorylation Q13535 (ATR)

研究背景

功能:

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

翻译修饰:

Phosphorylation seems to increase interaction with CDK4.

细胞定位:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific.

亚基结构:

Heterodimer with CDK4 or CDK6. Predominant p16 complexes contained CDK6. Interacts with CDK4 (both 'T-172'-phosphorylated and non-phosphorylated forms); the interaction inhibits cyclin D-CDK4 kinase activity. Interacts with ISCO2.

蛋白家族:

Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

文献引用

1). Heme oxygenase-1 prevents heart against myocardial infarction by attenuating ischemic injury-induced cardiomyocytes senescence. EBioMedicine, 2019 (PubMed: 30527623) [IF=11.1]

Application: IHC    Species: mouse    Sample: heart

Fig. 3. Up-regulation of HO-1 attenuated heart aging in vivo. The systemic HO-1 transgenic overexpression aged mice were 16 months. (a) The protein levels of LaminB, p53 and p16 were analyzed by western blot. n = 3. **P b .05. Data are mean ± SEM; one-way ANOVA was used for the statistical analysis. (b) Immunofluorescence staining using anti-p16 and anti-α-actinin antibodies showed the level change of p16. The tissue section thickness is 6 μm. Scale bars represent 100 μm. (c) Immunohistochemistry staining using anti-p16 antibody showed the level change of p16. The tissue section thickness is 6 μm. Scale bars represent 100 μm. (d) Relative levels of SASP containing IL-1, IL-3 and TNF-α were analyzed by qRT-PCR. n = 3 to 4. *P b .01. Data are mean ± SEM; Two-tailed t-test was used for the statistical analysis.

2). RETRACTED: lncRNA ILF3-AS1 promotes proliferation and metastasis of colorectal cancer cells by recruiting histone methylase EZH2. Molecular Therapy - Nucleic Acids, 2021 (PubMed: 34141456) [IF=8.8]

Application: WB    Species: Mouse    Sample:

FIGURE 2 PTS reduces reduction of NLRP3 inflammasome and apoptosis in DOX-treated mice. (A) WT C57/BL6J mice were pretreated with PTS (10 mg/kg) and afterwards with DOX (20 mg/kg cumulative dose) for 6 days. Immunochemistry staining of the liver sections with anti-NLRP3 (left, scale bar = 50 μm), the quantification of NLRP3 positive area in each group (right, n = 6); (B) qPCR analyses of NLRP3, IL-1β and IL-18 mRNA levels in each group (n = 6); (C) Western blot analyses of NLRP3, ASC, Caspase-1 p20, IL-1β, and IL-18 proteins and the quantification of the blots in each group (n = 4 per group); (D) Western blot analyses of Cleaved Caspase-3, BAX, and BCL-2 proteins (left) and quantification of the blots in each group (right, n = 4 per group).

3). Epigallocatechin gallate suppresses mitotic clonal expansion and adipogenic differentiation of preadipocytes through impeding JAK2/STAT3-mediated transcriptional cascades. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38552377) [IF=7.9]

4). Mechanical stretching can modify the papillary dermis pattern and papillary fibroblast characteristics during skin regeneration. Journal of Investigative Dermatology, 2022 (PubMed: 35181299) [IF=6.5]

5). Pathological and Prognostic Characterization of Craniopharyngioma Based on the Expression of TrkA, β-Catenin, Cell Cycle Markers, and BRAF V600E Mutation. Frontiers in Endocrinology, 2023 (PubMed: 35707464) [IF=5.2]

Application: IHC    Species: Human    Sample: neoplastic cells

Figure 3 β-Catenin, cyclin D1, P16, and Ki-67 expression in CP. (A–C) β-Catenin immunostaining score: (A) normal membranous staining in stratified squamous epithelial cells in PCP, (B) moderate and strong nuclear signal in 10%–50% and (C) >50% of neoplastic cells in two ACP cases, respectively. The signal was more represented in cells forming “whorled epithelium” structures. (D–F) Cyclin D1 immunostaining score: low nuclear signal observed in <10% of neoplastic cells (D, a case of PCP); moderate signal observed in 10%–50% of cells (E, a case of PCP); and strong reactivity in >50% of neoplastic cells (F, a case of ACP). (G–I) P16 immunostaining score: (G) signal with low intensity observed in <10% of neoplastic cells in PCP and (H) moderate intensity observed in 10%–50% of neoplastic cells in PCP; and (I) moderate and strong and diffuse (51%–80% of neoplastic cells) immunoreactivity in ACP. (J–L) Ki-67 labeling index: low proliferative index Ki-67 (<5%), and “whorled epithelium” structure (*) (J) extremely low Ki-67 index, (K) high Ki-67 index, and (I) Ki-67 index calculated as 15% in a case of PCP. All pictures were captured at 200× magnification.

6). Circular RNA NF1-419 enhances autophagy to ameliorate senile dementia by binding Dynamin-1 and Adaptor protein 2 B1 in AD-like mice. Aging, 2019 (PubMed: 31860870) [IF=5.2]

Application: WB    Species: Mouse    Sample:

Figure 1 Senescent astrocyte establishment and identification. (A–D) The astrocyte were treated with 20 g/L of D-galactose for different continuous passage culture time, and the senescent cells rate (bule staining cells) was detected using β-galactosidase staining; (E, F) The cells were treated with 20g/L of D-galactose for continuous passage culture of two generations, and the damaged DNA fragments was detected by single cell gel electrophoresis; (H–K) TUNEL (Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling) staining and DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) staining, DAPI stained all cells blue and TUNEL kits labeled apoptotic cells with green fluorescence; (L) The cell proliferation rate, (M) apoptosis rate and (N) cell cycle measurement after treated with 20 g/L of D-galactose for different continuous passage culture time; (O) Western blotting of P16INK4a and P21Cip1/Waf1 protein expressions; (P) The cell morphology with Giemsa staining at different time on the 20 g/L of D-galactose concentration. D0 (culture without 20 g/L D-galactose), D1 (culture in 20 g/L D-galactose for one generations) and D2 (culture in 20 g/L D-galactose for two generations) (Figure 2C, ​,2D),2D), D3 (culture in 20 g/L D-galactose for three generations). Data are presented as the means±SD of 3 independent experiments. *p

7). Myristic acid alleviates hippocampal aging correlated with GABAergic signaling. Frontiers in Nutrition, 2022 (PubMed: 36159502) [IF=5.0]

Application: IHC    Species: Mouse    Sample: hippocampus

Figure 2 Evaluation of hippocampal histomorphology in aging mice. (A) H&E and Nissl staining in the hippocampus between 3M and 22M group. The magnifications and scale bars were shown in (A). (B,C) Nissl positive cells and Neurodegeneration rate in the hippocampus were assessed by Nissl staining. Data were represented as mean ± SD and were analyzed by Student t-test. ***P < 0.001. Arrows showed degenerated neurons. (D) IHC of senescence markers P16 and P21 in the hippocampus. The magnifications and scale bars were shown in (D). (E,F) Relative expressions of senescence markers P16 and P21 were calculated by IHC. Data were represented as mean ± SD and were analyzed by Student t-test.

8). Plastrum testudinis Ameliorates Oxidative Stress in Nucleus Pulposus Cells via Downregulating the TNF-α Signaling Pathway. Pharmaceuticals (Basel, Switzerland), 2023 (PubMed: 37895953) [IF=4.6]

Application: WB    Species: Mouse    Sample: NPCs

Figure 5 Detection of p16, IL6, TNF-α, and ROS levels in NPCs. (A) Expression of p16 and IL-6 proteins in NPCs treated with TBHP, with or without PT. (B,C) Expression of p16 and IL-6 proteins relative to β-actin in NPCs treated with TBHP, with or without PT. (D) ROS production in NPCs treated with TBHP, with or without PT. (E) Expression of TNF-α protein in NPCs treated with LPS, with or without PT. (F) Expression of TNF-α protein relative to β-actin in NPCs treated with LPS, with or without PT. Data are the mean ± standard deviation. # p < 0.05, ## p < 0.01 vs. CTL group; * p < 0.05, ** p < 0.01 vs. 100 μM TBHP or 1 μg/mL LPS group.

9). P53 negatively regulates the osteogenic differentiation in jaw bone marrow MSCs derived from diabetic osteoporosis. Heliyon, 2023 (PubMed: 37096002) [IF=4.0]

Application: WB    Species: Human    Sample: hJBMMSCs

Fig. 6. Senescence characteristics of NC and DOP hJBMMSCs. A. The SA-β-Gal (senescence-associated β-galactosidase) expression in DOP group was significantly higher than that in NC group (Mean ± SD, n = 6). B. The mitochondrial green fluorescence in DOP group was significantly stronger than that in NC group, and the ratio of red/green fluorescence was significantly lower than that of NC group (Mean ± SD, n = 6). C. In the detection of reactive oxygen species, the fluorescence intensity of DOP group was significantly stronger than that of NC group (Mean ± SD, n = 6). D. The expression of senescence related secretory phenotype genes was increased in DOP group (Mean ± SD, n = 3). E. The expression of senescence related proteins in DOP group was increased (Mean ± SD, n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.

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