产品: MARCKS 抗体
货号: AF7789
描述: Rabbit polyclonal antibody to MARCKS
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Rabbit, Chicken
分子量: 31kDa; 32kD(Calculated).
蛋白号: P29966
RRID: AB_2844153

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Rabbit(90%), Chicken(89%)
克隆:
Polyclonal
特异性:
MARCKS Antibody detects endogenous levels of total MARCKS.
RRID:
AB_2844153
引用格式: Affinity Biosciences Cat# AF7789, RRID:AB_2844153.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

80 kDa protein; 80K L; 80K L protein; 80K-L protein; 80KL; 81 kDa protein, light chain; light chain; MACS; MARCKS; MARCS; MARCS_HUMAN; MGC52672; myristoylated alanine rich C kinase substrate; Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L); Myristoylated alanine rich protein kinase C substrate; Myristoylated alanine-rich C-kinase substrate; Phosphomyristin; PKCSL; PRKCSL; protein kinase C substrate 80 kDa protein light chain; Protein kinase C substrate;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MGAQFSKTAAKGEAAAERPGEAAVASSPSKANGQENGHVKVNGDASPAAAESGAKEELQANGSAPAADKEEPAAAGSGAASPSAAEKGEPAAAAAPEAGASPVEKEAPAEGEAAEPGSPTAAEGEAASAASSTSSPKAEDGATPSPSNETPKKKKKRFSFKKSFKLSGFSFKKNKKEAGEGGEAEAPAAEGGKDEAAGGAAAAAAEAGAASGEQAAAPGEEAAAGEEGAAGGDPQEAKPQEAAVAPEKPPASDETKAAEEPSKVEEKKAEEAGASAAACEAPSAAGPGAPPEQEAAPAEEPAAAAASSACAAPSQEAQPECSPEAPPAEAAE

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Rabbit
90
Chicken
89
Xenopus
78
Horse
0
Sheep
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P29966 作为底物

Site PTM Type Enzyme
G2 Myristoylation
K11 Ubiquitination
S26 Phosphorylation P24941 (CDK2)
S27 Phosphorylation P24941 (CDK2) , P17252 (PRKCA)
S29 Phosphorylation
K30 Ubiquitination
S46 Phosphorylation P17252 (PRKCA)
S52 Phosphorylation
K55 Ubiquitination
S63 Phosphorylation
K69 Ubiquitination
S77 Phosphorylation
S81 Phosphorylation P17252 (PRKCA)
S83 Phosphorylation
K87 Ubiquitination
S101 Phosphorylation P17252 (PRKCA)
K105 Ubiquitination
S118 Phosphorylation P17252 (PRKCA) , P24941 (CDK2)
T120 Phosphorylation
S128 Phosphorylation
S131 Phosphorylation P24941 (CDK2)
S132 Phosphorylation P24941 (CDK2)
T133 Phosphorylation P24941 (CDK2)
S134 Phosphorylation P24941 (CDK2)
S135 Phosphorylation P24941 (CDK2)
T143 Phosphorylation
S145 Phosphorylation
S147 Phosphorylation P24941 (CDK2)
T150 Phosphorylation P24941 (CDK2)
K152 Ubiquitination
K153 Ubiquitination
S159 Phosphorylation Q13464 (ROCK1) , P17252 (PRKCA) , P05771 (PRKCB) , P17612 (PRKACA) , Q16512 (PKN1)
K161 Methylation
S163 Phosphorylation P17252 (PRKCA) , Q16512 (PKN1) , P05771 (PRKCB)
K165 Ubiquitination
S167 Phosphorylation P17252 (PRKCA)
S170 Phosphorylation P17252 (PRKCA) , Q16512 (PKN1) , P05771 (PRKCB)
K172 Acetylation
K172 Methylation
K176 Ubiquitination
K248 Methylation
S252 Phosphorylation
S262 Phosphorylation
K267 Ubiquitination
S314 Phosphorylation

研究背景

功能:

MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

翻译修饰:

Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.

细胞定位:

Cytoplasm>Cytoskeleton. Membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the MARCKS family.

研究领域

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.