| 产品: | SREBP1 抗体 |
| 货号: | AF7799 |
| 描述: | Rabbit polyclonal antibody to SREBP1 |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| 分子量: | 122kDa; 122kD(Calculated). |
| 蛋白号: | P36956 |
| RRID: | AB_2844163 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7799, RRID:AB_2844163.
展开/折叠
ADD 1; bHLHd1; Class D basic helix-loop-helix protein 1; D630008H06; Processed sterol regulatory element-binding protein 1; SRBP1_HUMAN; SREBF 1; SREBF1; SREBP 1; SREBP 1c; SREBP-1; SREBP1; Sterol regulatory element binding protein 1; Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1; Sterol regulatory element binding transcription factor 1; Sterol regulatory element-binding transcription factor 1;
抗原和靶标
Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
- P36956 SRBP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDEPPFSEAALEQALGEPCDLDAALLTDIEDMLQLINNQDSDFPGLFDPPYAGSGAGGTDPASPDTSSPGSLSPPPATLSSSLEAFLSGPQAAPSPLSPPQPAPTPLKMYPSMPAFSPGPGIKEESVPLSILQTPTPQPLPGALLPQSFPAPAPPQFSSTPVLGYPSPPGGFSTGSPPGNTQQPLPGLPLASPPGVPPVSLHTQVQSVVPQQLLTVTAAPTAAPVTTTVTSQIQQVPVLLQPHFIKADSLLLTAMKTDGATVKAAGLSPLVSGTTVQTGPLPTLVSGGTILATVPLVVDAEKLPINRLAAGSKAPASAQSRGEKRTAHNAIEKRYRSSINDKIIELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLSLRTAVHKSKSLKDLVSACGSGGNTDVLMEGVKTEVEDTLTPPPSDAGSPFQSSPLSLGSRGSGSGGSGSDSEPDSPVFEDSKAKPEQRPSLHSRGMLDRSRLALCTLVFLCLSCNPLASLLGARGLPSPSDTTSVYHSPGRNVLGTESRDGPGWAQWLLPPVVWLLNGLLVLVSLVLLFVYGEPVTRPHSGPAVYFWRHRKQADLDLARGDFAQAAQQLWLALRALGRPLPTSHLDLACSLLWNLIRHLLQRLWVGRWLAGRAGGLQQDCALRVDASASARDAALVYHKLHQLHTMGKHTGGHLTATNLALSALNLAECAGDAVSVATLAEIYVAAALRVKTSLPRALHFLTRFFLSSARQACLAQSGSVPPAMQWLCHPVGHRFFVDGDWSVLSTPWESLYSLAGNPVDPLAQVTQLFREHLLERALNCVTQPNPSPGSADGDKEFSDALGYLQLLNSCSDAAGAPAYSFSISSSMATTTGVDPVAKWWASLTAVVIHWLRRDEEAAERLCPLVEHLPRVLQESERPLPRAALHSFKAARALLGCAKAESGPASLTICEKASGYLQDSLATTPASSSIDKAVQLFLCDLLLVVRTSLWRQQQPPAPAPAAQGTSSRPQASALELRGFQRDLSSLRRLAQSFRPAMRRVFLHEATARLMAGASPTRTHQLLDRSLRRRAGPGGKGGAVAELEPRPTRREHAEALLLASCYLPPGFLSAPGQRVGMLAEAARTLEKLGDRRLLHDCQQMLMRLGGGTTVTSS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P36956 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T59 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| S117 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) , P45983 (MAPK8) | Uniprot |
| K123 | Sumoylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| T275 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| R321 | Methylation | Uniprot | |
| K324 | Acetylation | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| K333 | Acetylation | Uniprot | |
| S337 | Phosphorylation | P57059 (SIK1) | Uniprot |
| S338 | Phosphorylation | P57059 (SIK1) , P17612 (PRKACA) | Uniprot |
| K342 | Ubiquitination | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| S396 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| K398 | Ubiquitination | Uniprot | |
| S402 | Phosphorylation | P57059 (SIK1) | Uniprot |
| K418 | Sumoylation | Uniprot | |
| T424 | Phosphorylation | Uniprot | |
| T426 | Phosphorylation | P49841 (GSK3B) , Q16539 (MAPK14) | Uniprot |
| S430 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S434 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S439 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S448 | Phosphorylation | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| S457 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K470 | Ubiquitination | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| Y567 | Phosphorylation | Uniprot | |
| K587 | Ubiquitination | Uniprot | |
| K675 | Ubiquitination | Uniprot | |
| K727 | Ubiquitination | Uniprot | |
| K924 | Methylation | Uniprot | |
| K924 | Ubiquitination | Uniprot | |
| K934 | Ubiquitination | Uniprot | |
| K947 | Ubiquitination | Uniprot | |
| S1020 | Phosphorylation | Uniprot | |
| S1027 | Phosphorylation | Uniprot | |
| S1049 | Phosphorylation | Uniprot | |
| S1060 | Phosphorylation | Uniprot | |
| K1070 | Ubiquitination | Uniprot | |
| K1121 | Ubiquitination | Uniprot |
研究背景
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding (By similarity).
Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle>COPII-coated vesicle membrane>Multi-pass membrane protein.
Note: Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Nucleus.
Nucleus.
Nucleus.
Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Interacts with CEBPA, the interaction produces a transcriptional synergy (By similarity).
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Belongs to the SREBP family.
研究领域
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.