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| 产品: | CrkL 抗体 |
| 货号: | AF7805 |
| 描述: | Rabbit polyclonal antibody to CrkL |
| 应用: | WB |
| 反应: | Human, Mouse, Rat, Monkey |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 39kDa; 34kD(Calculated). |
| 蛋白号: | P46109 |
| RRID: | AB_2844169 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7805, RRID:AB_2844169.
展开/折叠
Crk L; Crk like protein; Crk-like protein; Crkl; CRKL_HUMAN; Crkol; HGNC:2363; Oncogene CrkL; V crk avian sarcoma virus CT10 oncogene homolog like; v crk sarcoma virus CT10 oncogene homolog (avian) like; V crk sarcoma virus CT10 oncogene homolog avian like;
抗原和靶标
- P46109 CRKL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSARFDSSDRSAWYMGPVSRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPSPPMGSVSAPNLPTAEDNLEYVRTLYDFPGNDAEDLPFKKGEILVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPDENE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P46109 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R11 | Methylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| T43 | Phosphorylation | Uniprot | |
| C44 | S-Nitrosylation | Uniprot | |
| Y48 | Phosphorylation | Uniprot | |
| Y92 | Phosphorylation | Uniprot | |
| T95 | Phosphorylation | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| T97 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| Y127 | Phosphorylation | Uniprot | |
| T130 | Phosphorylation | Uniprot | |
| Y132 | Phosphorylation | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| Y177 | Phosphorylation | Uniprot | |
| K180 | Acetylation | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| Y198 | Phosphorylation | Uniprot | |
| Y207 | Phosphorylation | A9UF07 (BCR/ABL fusion) , A0A173G4P4 (Abl fusion) , P00519 (ABL1) | Uniprot |
| T212 | Phosphorylation | Uniprot | |
| T213 | Phosphorylation | Uniprot | |
| T214 | Phosphorylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| T228 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| Y251 | Phosphorylation | Uniprot | |
| K253 | Acetylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot |
研究背景
May mediate the transduction of intracellular signals.
Interacts with tyrosine-phosphorylated EPOR and INPP5D/SHIP1. Interacts with DOCK2 and DOCK5 via its first SH3 domain. Interacts with phosphorylated CBLB and IRS4.
Belongs to the CRK family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
文献引用
Application: WB Species: Human Sample: K562/ADR cells
Application: IHC Species: Human Sample: K562/ADR cells
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