| 产品: | p130 Cas 抗体 |
| 货号: | AF7826 |
| 描述: | Rabbit polyclonal antibody to p130 Cas |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus |
| 分子量: | 130kDa; 93kD(Calculated). |
| 蛋白号: | P56945 |
| RRID: | AB_2844190 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7826, RRID:AB_2844190.
展开/折叠
BCAR 1; Bcar1; BCAR1_HUMAN; Breast cancer anti estrogen resistance 1; Breast cancer anti estrogen resistance 1 protein; Breast cancer anti-estrogen resistance protein 1; CAS; Cas scaffolding protein family member 1; CAS1; Cass1; Crk associated substrate; Crk associated substrate p130Cas; CRK-associated substrate; CRKAS; FLJ12176; FLJ45059; p130cas;
抗原和靶标
Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
- P56945 BCAR1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPAGPGPGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPSPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREAPAEGKRLSASSTGSTRSSQSASSLEVAGPGREPLELEVAVEALARLQQGVSATVAHLLDLAGSAGATGSWRSPSEPQEPLVQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAKLSRQLQKMEDVHQTLVAHGQALDAGRGGSGATLEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKATAPGPEGGGTLHPNPTDKTSSIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLEKGSITRQGKSQLELQQLKQFERLEQEVSRPIDHDLANWTPAQPLAPGRTGGLGPSDRQLLLFYLEQCEANLTTLTNAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVERVKELGHSTQQFRRVLGQLAAA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P56945 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| Y12 | Phosphorylation | P51813 (BMX) | Uniprot |
| S18 | Phosphorylation | Uniprot | |
| Y67 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| Y115 | Phosphorylation | Uniprot | |
| Y128 | Phosphorylation | P11362 (FGFR1) , P12931 (SRC) | Uniprot |
| S134 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| K143 | Acetylation | Uniprot | |
| Y165 | Phosphorylation | Q13882 (PTK6) , Q07912 (TNK2) | Uniprot |
| Y179 | Phosphorylation | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| Y192 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| Y222 | Phosphorylation | Uniprot | |
| Y224 | Phosphorylation | Q13882 (PTK6) | Uniprot |
| Y234 | Phosphorylation | Q13882 (PTK6) | Uniprot |
| Y249 | Phosphorylation | Q13882 (PTK6) , P11362 (FGFR1) , P12931 (SRC) , Q07912 (TNK2) | Uniprot |
| S260 | Phosphorylation | Uniprot | |
| Y262 | Phosphorylation | Q13882 (PTK6) | Uniprot |
| Y267 | Phosphorylation | Q13882 (PTK6) | Uniprot |
| T269 | Phosphorylation | Uniprot | |
| Y287 | Phosphorylation | Q13882 (PTK6) | Uniprot |
| S292 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| Y306 | Phosphorylation | P11362 (FGFR1) , Q13882 (PTK6) | Uniprot |
| S311 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| T326 | Phosphorylation | Uniprot | |
| Y327 | Phosphorylation | P11362 (FGFR1) , Q13882 (PTK6) | Uniprot |
| S355 | Phosphorylation | Uniprot | |
| Y362 | Phosphorylation | Q13882 (PTK6) | Uniprot |
| Y372 | Phosphorylation | Uniprot | |
| T385 | Phosphorylation | Uniprot | |
| Y387 | Phosphorylation | Q13882 (PTK6) | Uniprot |
| S407 | Phosphorylation | Uniprot | |
| Y410 | Phosphorylation | Q05397 (PTK2) , P11362 (FGFR1) , Q07912 (TNK2) | Uniprot |
| S428 | Phosphorylation | Uniprot | |
| S430 | Phosphorylation | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| T435 | Phosphorylation | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| S440 | Phosphorylation | Uniprot | |
| S602 | Phosphorylation | Uniprot | |
| S639 | Phosphorylation | Uniprot | |
| S648 | Phosphorylation | Uniprot | |
| Y653 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y664 | Phosphorylation | Q13882 (PTK6) , P12931 (SRC) | Uniprot |
| Y666 | Phosphorylation | P12931 (SRC) | Uniprot |
| K693 | Ubiquitination | Uniprot | |
| S694 | Phosphorylation | Uniprot | |
| K702 | Ubiquitination | Uniprot | |
| Y815 | Phosphorylation | Uniprot | |
| K831 | Ubiquitination | Uniprot | |
| K851 | Ubiquitination | Uniprot |
研究背景
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
Dephosphorylated by PTPN14 at Tyr-128.
Cell junction>Focal adhesion. Cytoplasm.
Note: Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
Forms complexes in vivo with PTK2/FAK1, adapter protein CRKL and LYN kinase. Can heterodimerize with NEDD9. Interacts with BCAR3, the interaction regulates adhesion-dependent serine phosphorylation. Interacts with NPHP1 and SH2D3C. Interacts with activated CSPG4. Interacts with BMX, INPPL1/SHIP2 and PEAK1. Part of a collagen-stimulated complex involved in cell migration made of CDC42, CRK, TNK2 and BCAR1/p130cas. Interacts with TNK2 via SH3 domains. Interacts with PTK2B/PYK2.
Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9 (By similarity).
A serine-rich region promotes activation of the serum response element (SRE).
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
Belongs to the CAS family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.