| 产品: | GAB1 抗体 |
| 货号: | AF7873 |
| 描述: | Rabbit polyclonal antibody to GAB1 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 76.6kDa; 77kD(Calculated). |
| 蛋白号: | Q13480 |
| RRID: | AB_2844237 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7873, RRID:AB_2844237.
展开/折叠
Gab 1; GAB1; GAB1_HUMAN; GRB 2 associated binder 1; GRB 2 associated binding protein 1; GRB2 associated binding protein 1 isoform a; GRB2 associated binding protein 1 isoform b; GRB2-associated binder 1; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1;
抗原和靶标
- Q13480 GAB1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGGEVVCSGWLRKSPPEKKLKRYAWKRRWFVLRSGRLTGDPDVLEYYKNDHAKKPIRIIDLNLCQQVDAGLTFNKKEFENSYIFDINTIDRIFYLVADSEEEMNKWVRCICDICGFNPTEEDPVKPPGSSLQAPADLPLAINTAPPSTQADSSSATLPPPYQLINVPPHLETLGIQEDPQDYLLLINCQSKKPEPTRTHADSAKSTSSETDCNDNVPSHKNPASSQSKHGMNGFFQQQMIYDSPPSRAPSASVDSSLYNLPRSYSHDVLPKVSPSSTEADGELYVFNTPSGTSSVETQMRHVSISYDIPPTPGNTYQIPRTFPEGTLGQTSKLDTIPDIPPPRPPKPHPAHDRSPVETCSIPRTASDTDSSYCIPTAGMSPSRSNTISTVDLNKLRKDASSQDCYDIPRAFPSDRSSSLEGFHNHFKVKNVLTVGSVSSEELDENYVPMNPNSPPRQHSSSFTEPIQEANYVPMTPGTFDFSSFGMQVPPPAHMGFRSSPKTPPRRPVPVADCEPPPVDRNLKPDRKVKPAPLEIKPLPEWEELQAPVRSPITRSFARDSSRFPMSPRPDSVHSTTSSSDSHDSEENYVPMNPNLSSEDPNLFGSNSLDGGSSPMIKPKGDKQVEYLDLDLDSGKSTPPRKQKSSGSGSSVADERVDYVVVDQQKTLALKSTREAWTDGRQSTESETPAKSVK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13480 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| Y47 | Phosphorylation | Uniprot | |
| Y48 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| C213 | S-Nitrosylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| Y242 | Phosphorylation | P12931 (SRC) , P06213 (INSR) | Uniprot |
| S244 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| Y259 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y265 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| Y285 | Phosphorylation | P00533 (EGFR) , P06213 (INSR) , P08581 (MET) | Uniprot |
| S304 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| Y307 | Phosphorylation | P00533 (EGFR) , P08581 (MET) | Uniprot |
| T312 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| T316 | Phosphorylation | Uniprot | |
| Y317 | Phosphorylation | P12931 (SRC) | Uniprot |
| S355 | Phosphorylation | Uniprot | |
| T365 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| T369 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| S372 | Phosphorylation | Uniprot | |
| Y373 | Phosphorylation | P08581 (MET) , P06213 (INSR) , P00533 (EGFR) , P12931 (SRC) | Uniprot |
| S381 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S383 | Phosphorylation | Uniprot | |
| T387 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | Uniprot | |
| S401 | Phosphorylation | Uniprot | |
| S402 | Phosphorylation | Uniprot | |
| Y406 | Phosphorylation | P00533 (EGFR) , P08581 (MET) | Uniprot |
| S417 | Phosphorylation | Uniprot | |
| S418 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| Y447 | Phosphorylation | P06213 (INSR) , P08581 (MET) , P00533 (EGFR) | Uniprot |
| S454 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| Y472 | Phosphorylation | P00533 (EGFR) , P06213 (INSR) , P08581 (MET) | Uniprot |
| T476 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S500 | Phosphorylation | Uniprot | |
| T503 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| S582 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| Y589 | Phosphorylation | P06213 (INSR) , P00533 (EGFR) , P08581 (MET) | Uniprot |
| S597 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| Y627 | Phosphorylation | P06213 (INSR) , P12931 (SRC) , P08581 (MET) , P09619 (PDGFRB) , P00533 (EGFR) | Uniprot |
| S634 | Phosphorylation | Uniprot | |
| S637 | Phosphorylation | Uniprot | |
| T638 | Phosphorylation | Uniprot | |
| S645 | Phosphorylation | Uniprot | |
| S651 | Phosphorylation | Uniprot | |
| Y659 | Phosphorylation | P08581 (MET) , P06213 (INSR) , P00533 (EGFR) , P09619 (PDGFRB) | Uniprot |
| T673 | Phosphorylation | Uniprot | |
| S683 | Phosphorylation | Uniprot | |
| T684 | Phosphorylation | Uniprot |
研究背景
Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway.
Phosphorylated in response to FGFR1 activation. Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. Interacts with PTPRJ. Identified in a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Interacts (phosphorylated) with PTPN11. Interacts with HCK. Part of a tripartite complex containing GAB1, METTL13 and SPRY2. Interacts with METTL13.
Belongs to the GAB family.
研究领域
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
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