| 产品: | MLKL 抗体 |
| 货号: | AF7920 |
| 描述: | Rabbit polyclonal antibody to MLKL |
| 应用: | WB |
| 反应: | Human |
| 分子量: | 54kDa; 54kD(Calculated). |
| 蛋白号: | Q8NB16 |
| RRID: | AB_2844283 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7920, RRID:AB_2844283.
展开/折叠
9130019I15Rik; FLJ34389; hMLKL; Mixed lineage kinase domain like; Mixed lineage kinase domain like protein; Mixed lineage kinase domain like pseudokinase; Mixed lineage kinase domain-like protein; Mlkl; MLKL_HUMAN;
抗原和靶标
- Q8NB16 MLKL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQYLPPKCMQEIPQEQIKEIKKEQLSGSPWILLRENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKEIKTMKKFESPNILRIFGICIDETVTPPQFSIVMEYCELGTLRELLDREKDLTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQVKLAGFELRKTQTSMSLGTTREKTDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK
翻译修饰 - Q8NB16 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K40 | Ubiquitination | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| T59 | Phosphorylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| T246 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| T302 | Phosphorylation | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| K354 | Acetylation | Uniprot | |
| K354 | Ubiquitination | Uniprot | |
| T357 | Phosphorylation | Q9Y572 (RIPK3) | Uniprot |
| S358 | Phosphorylation | Q9Y572 (RIPK3) | Uniprot |
| T364 | Phosphorylation | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| T374 | Phosphorylation | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot |
研究背景
Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function.
Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.
Cytoplasm. Cell membrane.
Note: Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.
Homooligomer. Homotrimer; forms homotrimers on necroptosis induction. Interacts with RIPK3; the interaction is direct. Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3. Within this complex, may play a role in the proper targeting of RIPK1/RIPK3 to its downstream effector PGAM5.
The protein kinase domain is catalytically inactive but contains an unusual pseudoactive site with an interaction between Lys-230 and Gln-356 residues. Upon phosphorylation by RIPK3, undergoes an active conformation (By similarity).
The coiled coil region 2 is responsible for homotrimerization.
Belongs to the protein kinase superfamily.
研究领域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
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