(2)
(1)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7921, RRID:AB_2844284.
展开/折叠
hVps34; MGC61518; Phosphatidylinositol 3 kinase catalytic subunit type 3; Phosphatidylinositol 3 kinase class 3; Phosphatidylinositol 3 kinase p100 subunit; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide 3 kinase class 3; Phosphoinositide-3-kinase class 3; PI3 kinase type 3; PI3-kinase type 3; PI3K type 3; Pik3c3; PK3C3_HUMAN; PtdIns 3 kinase type 3; PtdIns-3-kinase type 3; Vps 34; Vps34;
抗原和靶标
- Q8NEB9 PK3C3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGEAEKFHYIYSCDLDINVQLKIGSLEGKREQKSYKAVLEDPMLKFSGLYQETCSDLYVTCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPRNAQVALTIWDVYGPGKAVPVGGTTVSLFGKYGMFRQGMHDLKVWPNVEADGSEPTKTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKRSSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDPQMSMENLVESKHHKLARSLRSGPSDHDLKPNAATRDQLNIIVSYPPTKQLTYEEQDLVWKFRYYLTNQEKALTKFLKCVNWDLPQEAKQALELLGKWKPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDEDLLMYLLQLVQALKYENFDDIKNGLEPTKKDSQSSVSENVSNSGINSAEIDSSQIITSPLPSVSSPPPASKTKEVPDGENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDQDTQQRDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVHLMKAVQRESGNRKKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGIIPETATLFKSALMPAQLFFKTEDGGKYPVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSIQNFFRKYAPSENGPNGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFHIDFGYILGRDPKPLPPPMKLNKEMVEGMGGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8NEB9 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S25 | Phosphorylation | Uniprot | |
| K29 | Acetylation | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| K36 | Methylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| T159 | Phosphorylation | P06493 (CDK1) , Q00535 (CDK5) | Uniprot |
| T163 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| Y231 | Phosphorylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| K287 | Methylation | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| T305 | Phosphorylation | Uniprot | |
| Y310 | Phosphorylation | Uniprot | |
| Y321 | Phosphorylation | Uniprot | |
| Y322 | Phosphorylation | Uniprot | |
| T324 | Phosphorylation | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| Y379 | Phosphorylation | Uniprot | |
| Y395 | Phosphorylation | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| K463 | Ubiquitination | Uniprot | |
| T488 | Phosphorylation | Uniprot | |
| K582 | Ubiquitination | Uniprot | |
| K600 | Ubiquitination | Uniprot | |
| K613 | Methylation | Uniprot | |
| T625 | Phosphorylation | Uniprot | |
| K630 | Ubiquitination | Uniprot | |
| Y631 | Phosphorylation | Uniprot | |
| K666 | Ubiquitination | Uniprot | |
| T668 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| T677 | Phosphorylation | Uniprot | |
| K706 | Ubiquitination | Uniprot | |
| K771 | Acetylation | Uniprot | |
| K781 | Acetylation | Uniprot | |
| K781 | Ubiquitination | Uniprot | |
| Y803 | Phosphorylation | Uniprot | |
| K840 | Sumoylation | Uniprot | |
| K845 | Sumoylation | Uniprot | |
| Y884 | Phosphorylation | Uniprot |
研究背景
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity).
Midbody. Late endosome. Cytoplasmic vesicle>Autophagosome.
Note: As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (Probable). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity).
Ubiquitously expressed, with a highest expression in skeletal muscle.
Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 associating with additional regulatory/auxilliary subunits to form alternative complex forms. Alternative complex forms containing a forth regulatory subunit in a mutually exclusive manner are: the PI3K complex I (PI3KC3-C1) containing ATG14, and the PI3K complex II (PI3KC3-C2) containing UVRAG. PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex. Both, PI3KC3-C1 and PI3KC3-C2, can associate with further regulatory subunits such as RUBCN, SH3GLB1/Bif-1 and AMBRA1. PI3KC3-C1 probably associates with PIK3CB (By similarity). Interacts with RAB7A in the presence of PIK3R4. Interacts with AMBRA1 (By similarity). Interacts with BECN1P1/BECN2. Interacts with SLAMF1. May be a component of a complex composed of RAB5A (in GDP-bound form), DYN2 and PIK3C3 (By similarity). Interacts with NCKAP1L.
Belongs to the PI3/PI4-kinase family.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
文献引用
Application: WB Species: rat Sample: spleen
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