PBK/TOPK Antibody - #AF7932

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产品: PBK/TOPK 抗体
货号: AF7932
描述: Rabbit polyclonal antibody to PBK/TOPK
应用: WB
反应: Human
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 36kDa; 36kD(Calculated).
蛋白号: Q96KB5
RRID: AB_2844295

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(92%)
克隆:
Polyclonal
特异性:
PBK/TOPK Antibody detects endogenous levels of total PBK/TOPK.
RRID:
AB_2844295
引用格式: Affinity Biosciences Cat# AF7932, RRID:AB_2844295.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cancer/testis antigen 84; CT84; Epididymis luminal protein 164; FLJ14385; HEL164; Lymphokine activated killer T cell originated protein kinase; Lymphokine-activated killer T-cell-originated protein kinase; MAPKK like protein kinase; MAPKK-like protein kinase; Nori 3; Nori-3; Nori3; PBK; PDZ binding kinase; PDZ-binding kinase; Serine/threonine protein kinase; Spermatogenesis related protein kinase; Spermatogenesis-related protein kinase; SPK; T LAK cell originated protein kinase; T-LAK cell-originated protein kinase; TOPK; TOPK_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q96KB5(TOPK_HUMAN) >>Visit HPA database.

基因/基因ID:
PBK(55872)
表达:
Q96KB5 TOPK_HUMAN:

Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.

序列:
MEGISNFKTPSKLSEKKKSVLCSTPTINIPASPFMQKLGFGTGVNVYLMKRSPRGLSHSPWAVKKINPICNDHYRSVYQKRLMDEAKILKSLHHPNIVGYRAFTEANDGSLCLAMEYGGEKSLNDLIEERYKASQDPFPAAIILKVALNMARGLKYLHQEKKLLHGDIKSSNVVIKGDFETIKICDVGVSLPLDENMTVTDPEACYIGTEPWKPKEAVEENGVITDKADIFAFGLTLWEMMTLSIPHINLSNDDDDEDKTFDESDFDDEAYYAALGTRPPINMEELDESYQKVIELFSVCTNEDPKDRPSAAHIVEALETDV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
92
Xenopus
92
Chicken
92
Zebrafish
78
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q96KB5 作为底物

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
K8 Sumoylation
K8 Ubiquitination
T9 Phosphorylation P06493 (CDK1)
S11 Phosphorylation
K12 Acetylation
K12 Sumoylation
K12 Ubiquitination
S14 Phosphorylation
K16 Acetylation
K17 Acetylation
S19 Phosphorylation
S23 Phosphorylation
T24 Phosphorylation
T26 Phosphorylation
S32 Phosphorylation
Y47 Phosphorylation
K50 Ubiquitination
S52 Phosphorylation
S57 Phosphorylation
S59 Phosphorylation
K64 Acetylation
K64 Sumoylation
K64 Ubiquitination
K65 Sumoylation
K65 Ubiquitination
Y74 Phosphorylation P12931 (SRC)
S76 Phosphorylation
Y78 Phosphorylation
K80 Ubiquitination
K87 Acetylation
K87 Ubiquitination
K90 Ubiquitination
S91 Phosphorylation
Y100 Phosphorylation
S122 Phosphorylation
K132 Ubiquitination
K155 Sumoylation
K155 Ubiquitination
K161 Ubiquitination
K162 Sumoylation
K162 Ubiquitination
K169 Sumoylation
K169 Ubiquitination
S171 Phosphorylation
K176 Sumoylation
K176 Ubiquitination
T181 Phosphorylation
T198 Phosphorylation
K213 Ubiquitination
T260 Phosphorylation
Y272 Phosphorylation P12931 (SRC)
S310 Phosphorylation

翻译修饰 - Q96KB5 作为激酶

Substrate Site Source
P05412 (JUN) S63 Uniprot
P05412 (JUN) S73 Uniprot
P25963 (NFKBIA) S32 Uniprot
P31749 (AKT1) S473 Uniprot
P68431 (HIST1H3J) S11 Uniprot
P81274 (GPSM2) T457 Uniprot
Q06830 (PRDX1) S32 Uniprot

研究背景

功能:

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.

翻译修饰:

Phosphorylated; in a cell-cycle dependent manner at mitosis.

组织特异性:

Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.

亚基结构:

Interacts with DLG1 and TP53.

蛋白家族:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

文献引用

1). Sharp Downregulation of Hub Genes Associated With the Pathogenesis of Breast Cancer From Ductal Carcinoma In Situ to Invasive Ductal Carcinoma. Frontiers in Oncology, 2021 (PubMed: 34094915) [IF=4.7]

Application: WB    Species: Human    Sample: breast tissue

Figure 6 Protein expression levels of hub genes. (A) Protein expression levels of CDK1, MELK, CEP55, TOP2A, NUSAP1, PBK, RRM2, and MAD2L1 were determined by western blotting. (B) Western blot analysis of CDK1, MELK, CEP55, TOP2A, NUSAP1, PBK, RRM2, and MAD2L1 in tissue from different stages of breast disease, and quantification of the intensity relative to GAPDH. One-way ANOVA was performed to acquire statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). NME, normal mammary epithelium; SH, simple ductal hyperplasia; ADH, atypical ductal hyperplasia; DCIS, ductal carcinoma in situ; IDC, invasive ductal carcinoma.

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