| 产品: | KIF5B 抗体 |
| 货号: | AF7947 |
| 描述: | Rabbit polyclonal antibody to KIF5B |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 110kDa; 110kD(Calculated). |
| 蛋白号: | P33176 |
| RRID: | AB_2844310 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7947, RRID:AB_2844310.
展开/折叠
Conventional kinesin heavy chain; KIF 5B; KIF5B; Kinesin 1; kinesin 1 (110-120kD); Kinesin 1 heavy chain; Kinesin family member 5B; Kinesin heavy chain; kinesin, heavy chain, ubiquitous; Kinesin-1 heavy chain; Kinesin1; KINH; KINH_HUMAN; KNS 1; KNS; KNS1; Ubiquitous kinesin heavy chain; UKHC;
抗原和靶标
- P33176 KINH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADLAECNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKILRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTVDKDITLTNDKPATAIGVIGNFTDAERRKCEEEIAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKDLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEENEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDALSEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKAKLITDLQDQNQKMMLEQERLRVEHEKLKATDQEKSRKLHELTVMQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEIDSDDTGGSAAQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENASRDRKRYQQEVDRIKEAVRSKNMARRGHSAQIAKPIRPGQHPAASPTHPSAIRGGGAFVQNSQPVAVRGGGGKQV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P33176 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K10 | Acetylation | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| K28 | Acetylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| C65 | S-Nitrosylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K166 | Acetylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| Y277 | Phosphorylation | Uniprot | |
| R321 | Methylation | Uniprot | |
| K346 | Acetylation | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| T397 | Phosphorylation | Uniprot | |
| T399 | Phosphorylation | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| T405 | Phosphorylation | Uniprot | |
| T414 | Phosphorylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K427 | Ubiquitination | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| T451 | Phosphorylation | Uniprot | |
| T463 | Phosphorylation | Uniprot | |
| R464 | Methylation | Uniprot | |
| K505 | Ubiquitination | Uniprot | |
| K514 | Ubiquitination | Uniprot | |
| Y516 | Phosphorylation | Uniprot | |
| K526 | Ubiquitination | Uniprot | |
| K539 | Ubiquitination | Uniprot | |
| K549 | Acetylation | Uniprot | |
| K560 | Acetylation | Uniprot | |
| Y593 | Phosphorylation | Uniprot | |
| S599 | Phosphorylation | Uniprot | |
| K602 | Acetylation | Uniprot | |
| K609 | Acetylation | Uniprot | |
| K609 | Ubiquitination | Uniprot | |
| C632 | S-Nitrosylation | Uniprot | |
| K644 | Ubiquitination | Uniprot | |
| S645 | Phosphorylation | Uniprot | |
| Y649 | Phosphorylation | Uniprot | |
| K656 | Ubiquitination | Uniprot | |
| K657 | Ubiquitination | Uniprot | |
| S663 | Phosphorylation | Uniprot | |
| K679 | Ubiquitination | Uniprot | |
| K690 | Ubiquitination | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| K761 | Acetylation | Uniprot | |
| K764 | Acetylation | Uniprot | |
| K764 | Ubiquitination | Uniprot | |
| K783 | Ubiquitination | Uniprot | |
| K791 | Ubiquitination | Uniprot | |
| K801 | Ubiquitination | Uniprot | |
| S814 | Phosphorylation | Uniprot | |
| S819 | Phosphorylation | Uniprot | |
| S825 | Phosphorylation | Uniprot | |
| S833 | Phosphorylation | Uniprot | |
| K844 | Ubiquitination | Uniprot | |
| K847 | Ubiquitination | Uniprot | |
| R901 | Methylation | Uniprot | |
| S917 | Phosphorylation | Uniprot | |
| K922 | Ubiquitination | Uniprot | |
| S933 | Phosphorylation | Uniprot | |
| T935 | Phosphorylation | Uniprot | |
| S938 | Phosphorylation | Uniprot | |
| S950 | Phosphorylation | Uniprot | |
| R956 | Methylation | Uniprot |
研究背景
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).
Cytoplasm>Cytoskeleton.
Note: Uniformly distributed between soma and neurites in hippocampal neurons.
Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 and PPP1R42 (By similarity). Interacts with SYBU. Interacts with JAKMIP1. Interacts with PLEKHM2. Interacts with ECPAS. Interacts with ZFYVE27 (By similarity). Found in a complex with OGT, RHOT1, RHOT2 and TRAK1. Interacts with APP (via cytoplasmic domain).
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Organismal Systems > Nervous system > Dopaminergic synapse.
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