DYRK1A/B Antibody - #DF10329

>>感恩教师节 已结束>>
(3)  
产品: DYRK1A/B 抗体
货号: DF10329
描述: Rabbit polyclonal antibody to DYRK1A/B
应用: WB
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 70KD; 86kD,69kD(Calculated).
蛋白号: Q13627 | Q9Y463
RRID: AB_2844336

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
DYRK1A/B Antibody detects endogenous levels of total DYRK1A/B.
RRID:
AB_2844336
引用格式: Affinity Biosciences Cat# DF10329, RRID:AB_2844336.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

;Dual specificity tyrosine phosphorylation regulated kinase; Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A; Dual specificity tyrosine phosphorylation regulated kinase 1; Dual specificity tyrosine phosphorylation regulated kinase 1A; Dual specificity tyrosine-phosphorylation-regulated kinase 1A; Dual specificity YAK 1 related kinase; Dual specificity YAK1 related kinase; Dual specificity YAK1-related kinase; DYR1A_HUMAN; DYRK 1; DYRK 1A; DYRK; DYRK1; Dyrk1a; DYRKA; hMNB; HP 86; HP86; Minibrain (Drosophila) homolog; Minibrain homolog; Minibrain, Drosophila, homolog of; MNB; MNB protein kinase; Mnb protein kinase homolog hp86; MNB protein kinase, Serine/threonine-specific; MNB/DYRK protein kinase; MNBH; MRD7; OTTHUMP00000109090; OTTHUMP00000109091; OTTHUMP00000109094; OTTHUMP00000174799; Protein kinase minibrain homolog; Serine/threonine kinase MNB; AOMS3; Dual specificity tyrosine (Y) phosphorylation regulated kinase 1B; Dual specificity tyrosine phosphorylation regulated kinase 1B; Dual specificity tyrosine-phosphorylation-regulated kinase 1B; DYR 1B; DYR1A; DYR1B; DYR1B_HUMAN; DYRK 1B; Dyrk1b; Minibrain related kinase; Minibrain-related kinase; MIRK; Mirk protein kinase;

抗原和靶标

免疫原:
Uniprot:

Q13627(DYR1A_HUMAN) >>Visit HPA database.

Q9Y463(DYR1B_HUMAN) >>Visit HPA database.

基因/基因ID:
DYRK1A(1859) | DYRK1B(9149)
表达:
Q13627 DYR1A_HUMAN:

Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.

Q9Y463 DYR1B_HUMAN:

Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.

序列:
MHTGGETSACKPSSVRLAPSFSFHAAGLQMAGQMPHSHQYSDRRQPNISDQQVSALSYSDQIQQPLTNQVMPDIVMLQRRMPQTFRDPATAPLRKLSVDLIKTYKHINEVYYAKKKRRHQQGQGDDSSHKKERKVYNDGYDDDNYDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQMCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGIPPAHILDQAPKARKFFEKLPDGTWNLKKTKDGKREYKPPGTRKLHNILGVETGGPGGRRAGESGHTVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSNSVSTSPAMEQSQSSGTTSSTSSSSGGSSGTSNSGRARSDPTHQHRHSGGHFTAAVQAMDCETHSPQVRQQFPAPLGWSGTEAPTQVTVETHPVQETTFHVAPQQNALHHHHGNSSHHHHHHHHHHHHHGQQALGNRTRPRVYNSPTNSSSTQDSMEVGHSHHSMTSLSSSTTSSSTSSSSTGNQGNQAYQNRPVAANTLDFGQNGAMDVNLTVYSNPRQETGIAGHPTYQFSANTGPAHYMTEGHLTMRQGADREESPMTGVCVQQSPVASS

MAVPPGHGPFSGFPGPQEHTQVLPDVRLLPRRLPLAFRDATSAPLRKLSVDLIKTYKHINEVYYAKKKRRAQQAPPQDSSNKKEKKVLNHGYDDDNHDYIVRSGERWLERYEIDSLIGKGSFGQVVKAYDHQTQELVAIKIIKNKKAFLNQAQIELRLLELMNQHDTEMKYYIVHLKRHFMFRNHLCLVFELLSYNLYDLLRNTHFRGVSLNLTRKLAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGTPYDLAIDMWSLGCILVEMHTGEPLFSGSNEVDQMNRIVEVLGIPPAAMLDQAPKARKYFERLPGGGWTLRRTKELRKDYQGPGTRRLQEVLGVQTGGPGGRRAGEPGHSPADYLRFQDLVLRMLEYEPAARISPLGALQHGFFRRTADEATNTGPAGSSASTSPAPLDTCPSSSTASSISSSGGSSGSSSDNRTYRYSNRYCGGPGPPITDCEMNSPQVPPSQPLRPWAGGDVPHKTHQAPASASSLPGTGAQLPPQPRYLGRPPSPTSPPPPELMDVSLVGGPADCSPPHPAPAPQHPAASALRTRMTGGRPPLPPPDDPATLGPHLGLRGVPQSTAASS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13627/Q9Y463 作为底物

Site PTM Type Enzyme
S49 Phosphorylation
K57 Acetylation
Y63 Phosphorylation
Y64 Phosphorylation
Y111 Phosphorylation
K119 Ubiquitination
K127 Ubiquitination
K256 Ubiquitination
Y271 Phosphorylation Q9Y463 (DYRK1B)
Y273 Phosphorylation Q9Y463 (DYRK1B)
S276 Phosphorylation
S421 Phosphorylation Q9Y463 (DYRK1B) , P28482 (MAPK1)
Y485 Phosphorylation
S486 Phosphorylation
S576 Phosphorylation
Site PTM Type Enzyme
K11 Ubiquitination
S14 Phosphorylation
S22 Phosphorylation
S97 Phosphorylation
Y104 Phosphorylation Q13627 (DYRK1A)
K105 Acetylation
Y111 Phosphorylation Q13627 (DYRK1A)
Y112 Phosphorylation
S127 Phosphorylation
K134 Ubiquitination
Y136 Phosphorylation
Y140 Phosphorylation Q13627 (DYRK1A)
Y145 Phosphorylation
Y147 Phosphorylation
K150 Ubiquitination
Y159 Phosphorylation Q13627 (DYRK1A)
K167 Ubiquitination
Y177 Phosphorylation Q13627 (DYRK1A)
K304 Ubiquitination
S310 Phosphorylation Q13627 (DYRK1A)
Y319 Phosphorylation Q13627 (DYRK1A)
Y321 Phosphorylation Q13627 (DYRK1A)
S324 Phosphorylation
S329 Phosphorylation
T402 Phosphorylation Q13627 (DYRK1A)
Y449 Phosphorylation Q13627 (DYRK1A)
K453 Ubiquitination
T466 Phosphorylation
S529 Phosphorylation Q13627 (DYRK1A)
T532 Phosphorylation
S538 Phosphorylation
T543 Phosphorylation
T553 Phosphorylation
S555 Phosphorylation
Y705 Phosphorylation
S706 Phosphorylation
S748 Phosphorylation
S758 Phosphorylation
S762 Phosphorylation

翻译修饰 - Q13627/Q9Y463 作为激酶

Substrate Site Source
O75533 (SF3B1) T434 Uniprot
P20823 (HNF1A) S249 Uniprot
P24385 (CCND1) T286 Uniprot
P24385 (CCND1) T288 Uniprot
P38936 (CDKN1A) S153 Uniprot
P46527 (CDKN1B) S10 Uniprot
Q02363 (ID2) T27 Uniprot
Q99801 (NKX3-1) S185 Uniprot
Q9UQL6 (HDAC5) S279 Uniprot
Q9Y463 (DYRK1B) Y271 Uniprot
Q9Y463 (DYRK1B) Y273 Uniprot
Q9Y463 (DYRK1B) S421 Uniprot
Substrate Site Source
O00401 (WASL) T199 Uniprot
O00401 (WASL) T205 Uniprot
O00401 (WASL) T262 Uniprot
O43524 (FOXO3) S330 Uniprot
O43597 (SPRY2) T75 Uniprot
O75533 (SF3B1) T273 Uniprot
O75533 (SF3B1) T434 Uniprot
P01106 (MYC) S62 Uniprot
P04637 (TP53) S15 Uniprot
P10636-8 (MAPT) S199 Uniprot
P10636-8 (MAPT) S202 Uniprot
P10636-8 (MAPT) T205 Uniprot
P10636-8 (MAPT) T212 Uniprot
P10636-8 (MAPT) T217 Uniprot
P10636 (MAPT) S369 Uniprot
P10636-8 (MAPT) S396 Uniprot
P10636-8 (MAPT) S404 Uniprot
P10636-8 (MAPT) S422 Uniprot
P10636 (MAPT) S519 Uniprot
P10636 (MAPT) T529 Uniprot
P10636 (MAPT) S721 Uniprot
P16220 (CREB1) S133 Uniprot
P24385 (CCND1) T286 Uniprot
P24928 (POLR2A) S1616 Uniprot
P24928 (POLR2A) S1619 Uniprot
P37840 (SNCA) S87 Uniprot
P46821 (MAP1B) S1400 Uniprot
P49418 (AMPH) S262 Uniprot
P49418 (AMPH) S272 Uniprot
P49418 (AMPH) S276 Uniprot
P49418 (AMPH) S285 Uniprot
P49418 (AMPH) S293 Uniprot
P49418 (AMPH) S295 Uniprot
P49418 (AMPH) T310 Uniprot
P49418 (AMPH) T312 Uniprot
P49768 (PSEN1) T354 Uniprot
P53805 (RCAN1) S167 Uniprot
P53805 (RCAN1) T247 Uniprot
P55211 (CASP9) T125 Uniprot
P61764 (STXBP1) T479 Uniprot
Q05193 (DNM1) S795 Uniprot
Q05193 (DNM1) S857 Uniprot
Q07955 (SRSF1) S227 Uniprot
Q07955 (SRSF1) S234 Uniprot
Q07955 (SRSF1) S238 Uniprot
Q12778 (FOXO1) S329 Uniprot
Q13627 (DYRK1A) Y104 Uniprot
Q13627 (DYRK1A) Y111 Uniprot
Q13627 (DYRK1A) Y140 Uniprot
Q13627 (DYRK1A) Y159 Uniprot
Q13627 (DYRK1A) Y177 Uniprot
Q13627 (DYRK1A) S310 Uniprot
Q13627 (DYRK1A) Y319 Uniprot
Q13627 (DYRK1A) Y321 Uniprot
Q13627 (DYRK1A) T402 Uniprot
Q13627 (DYRK1A) Y449 Uniprot
Q13627-1 (DYRK1A) S519 Uniprot
Q13627-2 (DYRK1A) S520 Uniprot
Q13627 (DYRK1A) S529 Uniprot
Q49AN0 (CRY2) S558 Uniprot
Q52LA3 (LIN52) S28 Uniprot
Q96EB6 (SIRT1) T530 Uniprot
Q96S94 (CCNL2) S330 Uniprot
Q96S94-1 (CCNL2) S338 Uniprot
Q96S94-1 (CCNL2) S369 Uniprot

研究背景

功能:

Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity). Exhibits a substrate preference for proline at position P+1 and arginine at position P-3. Has pro-survival function and negatively regulates the apoptotic process. Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. This in turn inhibits TP53 activity and apoptosis (By similarity).

翻译修饰:

Autophosphorylated on numerous tyrosine residues. Can also autophosphorylate on serine and threonine residues (in vitro).

细胞定位:

Nucleus. Nucleus speckle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.

亚基结构:

Interacts RAD54L2/ARIP4 (By similarity). Interacts with CRY2 (By similarity). Interacts with RANBP9. Interacts with WDR68. Interacts with SRSF6. Interacts with SIRT1 (By similarity).

(Microbial infection) Interacts with human adenovirus 5 E1A protein.

蛋白家族:

The polyhistidine repeats act as targeting signals to nuclear speckles.

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.

功能:

Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. Enhances the transcriptional activity of TCF1/HNF1A and FOXO1. Inhibits epithelial cell migration. Mediates colon carcinoma cell survival in mitogen-poor environments. Inhibits the SHH and WNT1 pathways, thereby enhancing adipogenesis. In addition, promotes expression of the gluconeogenic enzyme glucose-6-phosphatase (G6PC).

翻译修饰:

Autophosphorylated on tyrosine residues. Phosphorylated by MAP kinase. Tyrosine phosphorylation may be required for dimerization.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.

亚基结构:

Dimer. Interacts with DCOHM, MAP2K3/MKK3, RANBP9 and TCF1/HNF1A. Part of a complex consisting of RANBP9, RAN, DYRK1B and COPS5. Interacts with DCAF7.

蛋白家族:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.