| 产品: | MAP3K2 抗体 |
| 货号: | DF10336 |
| 描述: | Rabbit polyclonal antibody to MAP3K2 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 70kDa; 70kD(Calculated). |
| 蛋白号: | Q9Y2U5 |
| RRID: | AB_2844343 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF10336, RRID:AB_2844343.
展开/折叠
M3K2_HUMAN; Map3k2; MAPK/ERK kinase kinase 2; MEK kinase 2; MEKK 2; MEKK2b; Mitogen activated protein kinase kinase kinase 2; Mitogen-activated protein kinase kinase kinase 2;
抗原和靶标
- Q9Y2U5 M3K2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDQQALNSIMQDLAVLHKASRPALSLQETRKAKSSSPKKQNDVRVKFEHRGEKRILQFPRPVKLEDLRSKAKIAFGQSMDLHYTNNELVIPLTTQDDLDKAVELLDRSIHMKSLKILLVINGSTQATNLEPLPSLEDLDNTVFGAERKKRLSIIGPTSRDRSSPPPGYIPDELHQVARNGSFTSINSEGEFIPESMDQMLDPLSLSSPENSGSGSCPSLDSPLDGESYPKSRMPRAQSYPDNHQEFSDYDNPIFEKFGKGGTYPRRYHVSYHHQEYNDGRKTFPRARRTQGTSLRSPVSFSPTDHSLSTSSGSSIFTPEYDDSRIRRRGSDIDNPTLTVMDISPPSRSPRAPTNWRLGKLLGQGAFGRVYLCYDVDTGRELAVKQVQFDPDSPETSKEVNALECEIQLLKNLLHERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANILRDSTGNVKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPHVSDYTRDFLKRIFVEAKLRPSADELLRHMFVHYH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y2U5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S26 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| Y169 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| Y240 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| Y250 | Phosphorylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K260 | Methylation | Uniprot | |
| T263 | Phosphorylation | Uniprot | |
| T283 | Phosphorylation | Uniprot | |
| T290 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| T310 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| T337 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| K498 | Ubiquitination | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| K506 | Ubiquitination | Uniprot | |
| S514 | Phosphorylation | Uniprot | |
| T516 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| T522 | Phosphorylation | Uniprot | |
| T524 | Phosphorylation | Uniprot | |
| K581 | Ubiquitination | Uniprot | |
| K602 | Ubiquitination | Uniprot |
研究背景
Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.
Autophosphorylated.
Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation.
Cytoplasm. Nucleus.
Note: Upon EGF stimulation, translocates into the nucleus.
Interacts with PKN2; the interaction activates PKN2 kinase activity in a MAP3K2-independent kinase activity (By similarity). Self-associates. Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts (via the kinase catalytic domain) with STK38. Interacts with XIAP/BIRC4.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
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