产品: | 磷酸化 GEF H1 (Ser886) 抗体 |
货号: | AF4483 |
描述: | Rabbit polyclonal antibody to Phospho-GEF H1 (Ser886) |
应用: | WB IHC |
反应: | Human, Mouse, Rat |
预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
分子量: | 120kDa; 112kD(Calculated). |
蛋白号: | Q92974 |
RRID: | AB_2844534 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4483, RRID:AB_2844534.
展开/折叠
AA408978; ARHG2; ARHG2_HUMAN; ARHGEF 2; ARHGEF-2; ARHGEF2; GEF; GEF H1; GEF-H1; GEFH1; Guanine nucleotide exchange factor H1; KIAA0651; Lbcl1; Lfc; LFP40; MGC95068; Microtubule-regulated Rho-GEF; mKIAA0651; P40; Proliferating cell nucleolar antigen p40; Protein GEF-H1; Rho guanine nucleotide exchange factor 2; rho/rac guanine nucleotide exchange factor (GEF) 2; rho/rac guanine nucleotide exchange factor 2; rho/rac guanine nucleotide exchange factor;
抗原和靶标
- Q92974 ARHG2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRIESLTRARIDRSRELASKTREKEKMKEAKDARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCTKVKQKQQKAALLKNNTALQSVSLRSKTTIRERPSSAIYPSDSFRQSLLGSRRGRSSLSLAKSVSTTNIAGHFNDESPLGLRRILSQSTDSLNMRNRTLSVESLIDEAEVIYSELMSDFEMDEKDFAADSWSLAVDSSFLQQHKKEVMKQQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELHLEPGVVQGLFPCVDELSDIHTRFLSQLLERRRQALCPGSTRNFVIHRLGDLLISQFSGPSAEQMCKTYSEFCSRHSKALKLYKELYARDKRFQQFIRKVTRPAVLKRHGVQECILLVTQRITKYPLLISRILQHSHGIEEERQDLTTALGLVKELLSNVDEGIYQLEKGARLQEIYNRMDPRAQTPVPGKGPFGREELLRRKLIHDGCLLWKTATGRFKDVLVLLMTDVLVFLQEKDQKYIFPTLDKPSVVSLQNLIVRDIANQEKGMFLISAAPPEMYEVHTASRDDRSTWIRVIQQSVRTCPSREDFPLIETEDEAYLRRIKMELQQKDRALVELLREKVGLFAEMTHFQAEEDGGSGMALPTLPRGLFRSESLESPRGERLLQDAIREVEGLKDLLVGPGVELLLTPREPALPLEPDSGGNTSPGVTANGEARTFNGSIELCRADSDSSQRDRNGNQLRSPQEEALQRLVNLYGLLHGLQAAVAQQDTLMEARFPEGPERREKLCRANSRDGEAGRAGAAPVAPEKQATELALLQRQHALLQEELRRCRRLGEERATEAGSLEARLRESEQARALLEREAEEARRQLAALGQTEPLPAEAPWARRPVDPRRRSLPAGDALYLSFNPPQPSRGTDRLDLPVTTRSVHRNFEDRERQELGSPEERLQDSSDPDTGSEEEGSSRLSPPHSPRDFTRMQDIPEETESRDGEAVASES
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q92974 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S2 | Phosphorylation | Uniprot | |
S6 | Phosphorylation | Uniprot | |
T8 | Phosphorylation | Uniprot | |
K63 | Ubiquitination | Uniprot | |
K80 | Ubiquitination | Uniprot | |
K88 | Ubiquitination | Uniprot | |
K95 | Ubiquitination | Uniprot | |
K100 | Methylation | Uniprot | |
K100 | Ubiquitination | Uniprot | |
S109 | Phosphorylation | Uniprot | |
T115 | Phosphorylation | Uniprot | |
S121 | Phosphorylation | Uniprot | |
S122 | Phosphorylation | Uniprot | |
Y125 | Phosphorylation | Uniprot | |
S127 | Phosphorylation | Uniprot | |
S129 | Phosphorylation | Uniprot | |
S133 | Phosphorylation | Uniprot | |
S137 | Phosphorylation | Uniprot | |
S142 | Phosphorylation | Uniprot | |
S143 | Phosphorylation | Q7KZI7 (MARK2) | Uniprot |
S145 | Phosphorylation | Uniprot | |
K148 | Ubiquitination | Uniprot | |
S149 | Phosphorylation | Uniprot | |
S151 | Phosphorylation | Uniprot | |
T152 | Phosphorylation | Uniprot | |
T153 | Phosphorylation | Uniprot | |
S163 | Phosphorylation | Uniprot | |
S172 | Phosphorylation | Q7KZI7 (MARK2) , O14757 (CHEK1) | Uniprot |
S174 | Phosphorylation | Uniprot | |
T175 | Phosphorylation | Uniprot | |
S177 | Phosphorylation | Uniprot | |
T184 | Phosphorylation | Uniprot | |
S186 | Phosphorylation | Q7KZI7 (MARK2) | Uniprot |
S189 | Phosphorylation | Uniprot | |
K230 | Ubiquitination | Uniprot | |
K235 | Ubiquitination | Uniprot | |
K255 | Ubiquitination | Uniprot | |
S295 | Phosphorylation | Uniprot | |
S309 | Phosphorylation | Uniprot | |
T310 | Phosphorylation | Uniprot | |
S330 | Phosphorylation | Uniprot | |
K336 | Ubiquitination | Uniprot | |
K347 | Ubiquitination | Uniprot | |
K353 | Acetylation | Uniprot | |
K353 | Ubiquitination | Uniprot | |
Y356 | Phosphorylation | Uniprot | |
K376 | Methylation | Uniprot | |
C383 | S-Nitrosylation | Uniprot | |
T392 | Phosphorylation | Uniprot | |
K393 | Ubiquitination | Uniprot | |
Y394 | Phosphorylation | Uniprot | |
S427 | Phosphorylation | Uniprot | |
Y434 | Phosphorylation | Uniprot | |
K438 | Ubiquitination | Uniprot | |
T455 | Phosphorylation | Uniprot | |
K460 | Ubiquitination | Uniprot | |
K472 | Ubiquitination | Uniprot | |
K482 | Acetylation | Uniprot | |
K482 | Ubiquitination | Uniprot | |
K509 | Ubiquitination | Uniprot | |
Y510 | Phosphorylation | Uniprot | |
K517 | Ubiquitination | Uniprot | |
S519 | Phosphorylation | Uniprot | |
S522 | Phosphorylation | Uniprot | |
K594 | Ubiquitination | Uniprot | |
K600 | Ubiquitination | Uniprot | |
S643 | Phosphorylation | Uniprot | |
S645 | Phosphorylation | Uniprot | |
S648 | Phosphorylation | Uniprot | |
K666 | Ubiquitination | Uniprot | |
T679 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
S691 | Phosphorylation | Uniprot | |
T695 | Phosphorylation | Uniprot | |
S696 | Phosphorylation | Uniprot | |
S711 | Phosphorylation | Uniprot | |
C715 | S-Nitrosylation | Uniprot | |
S719 | Phosphorylation | Uniprot | |
S721 | Phosphorylation | Uniprot | |
S722 | Phosphorylation | Uniprot | |
S782 | Phosphorylation | Uniprot | |
K799 | Acetylation | Uniprot | |
K799 | Ubiquitination | Uniprot | |
T802 | Phosphorylation | Uniprot | |
T830 | Phosphorylation | Uniprot | |
S886 | Phosphorylation | Q16512 (PKN1) , O14965 (AURKA) , Q13153 (PAK1) , Q7KZI7 (MARK2) , O96013 (PAK4) | Uniprot |
Y894 | Phosphorylation | Uniprot | |
S896 | Phosphorylation | Uniprot | |
S903 | Phosphorylation | Uniprot | |
R904 | Methylation | Uniprot | |
S932 | Phosphorylation | Uniprot | |
S940 | Phosphorylation | Uniprot | |
S941 | Phosphorylation | Uniprot | |
T945 | Phosphorylation | Uniprot | |
S947 | Phosphorylation | Uniprot | |
S952 | Phosphorylation | Uniprot | |
S953 | Phosphorylation | Uniprot | |
S956 | Phosphorylation | Uniprot | |
S960 | Phosphorylation | Q7KZI7 (MARK2) | Uniprot |
S976 | Phosphorylation | Uniprot |
研究背景
Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability (By similarity). Involved in neuronal progenitor cell division and differentiation. Involved in the migration of precerebellar neurons (By similarity).
Phosphorylation of Ser-886 by PAK1 induces binding to protein YWHAZ, promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by AURKA and CDK1 during mitosis, which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules. Phosphorylated on serine, threonine and tyrosine residues in a RIPK2-dependent manner.
Cytoplasm>Cytoskeleton. Cytoplasm. Cell junction>Tight junction. Golgi apparatus. Cytoplasm>Cytoskeleton>Spindle. Cell projection>Ruffle membrane. Cytoplasmic vesicle.
Note: Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization (PubMed:15827085). Recruited into membrane ruffles induced by S.flexneri at tight junctions of polarized epithelial cells (PubMed:19043560). Colocalized with NOD2 and RIPK2 in vesicles and with the cytoskeleton (PubMed:21887730).
Found in a complex composed at least of ARHGEF2, NOD2 and RIPK2. Interacts with RIPK2; the interaction mediates tyrosine phosphorylation of RIPK2 by Src kinase CSK. Interacts with RIPK1 and RIPK3. Interacts with YWHAZ/14-3-3 zeta; when phosphorylated at Ser-886. Interacts with the kinases PAK4, AURKA and MAPK1. Interacts with RHOA and RAC1. Interacts with NOD1. Interacts (via the N-terminal zinc finger) with CAPN6 (via domain II). Interacts with DYNLT1 (By similarity).
The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA. Promotes tyrosine phosphorylation of RIPK2.
The PH domain has no affinity for phosphoinositides suggesting that it does not interact directly with membranes.
The phorbol-ester/DAG-type zinc-finger and the C-terminal coiled-coil domains (606-986) are both important for association with microtubules.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
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