| 产品: | DEPTOR 抗体 |
| 货号: | AF4613 |
| 描述: | Rabbit polyclonal antibody to DEPTOR |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 48kDa; 46kD(Calculated). |
| 蛋白号: | Q8TB45 |
| RRID: | AB_2844563 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4613, RRID:AB_2844563.
展开/折叠
DEP domain containing 6; DEP domain-containing mTOR-interacting protein; DEP domain-containing protein 6; DEP.6; DEPDC6; DEPTOR; DPTOR_HUMAN;
抗原和靶标
- Q8TB45 DPTOR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEEGGSTGSAGSDSSTSGSGGAQQRELERMAEVLVTGEQLRLRLHEEKVIKDRRHHLKTYPNCFVAKELIDWLIEHKEASDRETAIKLMQKLADRGIIHHVCDEHKEFKDVKLFYRFRKDDGTFPLDNEVKAFMRGQRLYEKLMSPENTLLQPREEEGVKYERTFMASEFLDWLVQEGEATTRKEAEQLCHRLMEHGIIQHVSNKHPFVDSNLLYQFRMNFRRRRRLMELLNEKSPSSQETHDSPFCLRKQSHDNRKSTSFMSVSPSKEIKIVSAVRRSSMSSCGSSGYFSSSPTLSSSPPVLCNPKSVLKRPVTSEELLTPGAPYARKTFTIVGDAVGWGFVVRGSKPCHIQAVDPSGPAAAAGMKVCQFVVSVNGLNVLHVDYRTVSNLILTGPRTIVMEVMEELEC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8TB45 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| T7 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| T59 | Phosphorylation | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| T149 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| K160 | Ubiquitination | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| T241 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| T259 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| S265 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S267 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S286 | Phosphorylation | P48729 (CSNK1A1) , Q15418 (RPS6KA1) , P42345 (MTOR) , P23443 (RPS6KB1) | Uniprot |
| S287 | Phosphorylation | Q15418 (RPS6KA1) , P48729 (CSNK1A1) , P23443 (RPS6KB1) | Uniprot |
| S291 | Phosphorylation | P48729 (CSNK1A1) , P23443 (RPS6KB1) , Q15418 (RPS6KA1) | Uniprot |
| S292 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | P42345 (MTOR) | Uniprot |
| T295 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S297 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | P42345 (MTOR) | Uniprot |
| K311 | Ubiquitination | Uniprot | |
| T315 | Phosphorylation | Uniprot | |
| T321 | Phosphorylation | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| T387 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | Uniprot | |
| T394 | Phosphorylation | Uniprot |
研究背景
Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.
Phosphorylated. Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2.
Ubiquitinated; ubiquitination leads to proteasomal degradation.
Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PROTOR1, RICTOR, MAPKAP1 and DEPTOR. Interacts (via PDZ domain) with MTOR; interacts with MTOR within both mammalian target of rapamycin complexes mTORC1 and mTORC2. Interacts (via PDZ domain) with MINAR1 (via N-terminus). Interacts with SIK3.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
限制条款
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